Details of the Target

General Information of Target

Target ID LDTP04541
Target Name Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2 (NDST2)
Gene Name NDST2
Gene ID 8509
Synonyms
HSST2; Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2; EC 2.8.2.8; Glucosaminyl N-deacetylase/N-sulfotransferase 2; NDST-2; N-heparan sulfate sulfotransferase 2; N-HSST 2) [Includes: Heparan sulfate N-deacetylase 2; EC 3.-.-.-; Heparan sulfate N-sulfotransferase 2; EC 2.8.2.-)]
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MLQLWKVVRPARQLELHRLILLLIAFSLGSMGFLAYYVSTSPKAKEPLPLPLGDCSSGGA
AGPGPARPPVPPRPPRPPETARTEPVVLVFVESAYSQLGQEIVAILESSRFRYSTELAPG
RGDMPTLTDNTHGRYVLVIYENLLKYVNLDAWSRELLDRYCVEYGVGIIGFFRAHEHSLL
SAQLKGFPLFLHSNLGLRDYQVNPSAPLLHLTRPSRLEPGPLPGDDWTIFQSNHSTYEPV
LLASLRPAEPAVPGPVLRRARLPTVVQDLGLHDGIQRVLFGHGLSFWLHKLIFVDAVAYL
TGKRLCLDLDRYILVDIDDIFVGKEGTRMKVADVEALLTTQNKLRTLVPNFTFNLGFSGK
FYHTGTEEEDAGDDMLLKHRKEFWWFPHMWSHMQPHLFHNRSVLADQMRLNKQFALEHGI
PTDLGYAVAPHHSGVYPIHTQLYEAWKSVWGIQVTSTEEYPHLRPARYRRGFIHNGIMVL
PRQTCGLFTHTIFYNEYPGGSRELDRSIRGGELFLTVLLNPISIFMTHLSNYGNDRLGLY
TFESLVRFLQCWTRLRLQTLPPVPLAQKYFELFPQERSPLWQNPCDDKRHKDIWSKEKTC
DRLPKFLIVGPQKTGTTAIHFFLSLHPAVTSSFPSPSTFEEIQFFNSPNYHKGIDWYMDF
FPVPSNASTDFLFEKSATYFDSEVVPRRGAALLPRAKIITVLTNPADRAYSWYQHQRAHG
DPVALNYTFYQVISASSQTPLALRSLQNRCLVPGYYSTHLQRWLTYYPSGQLLIVDGQEL
RTNPAASMESIQKFLGITPFLNYTRTLRFDDDKGFWCQGLEGGKTRCLGRSKGRRYPDMD
TESRLFLTDFFRNHNLELSKLLSRLGQPVPSWLREELQHSSLG
Target Bioclass
Enzyme
Family
Sulfotransferase 1 family, NDST subfamily
Subcellular location
Golgi apparatus membrane
Function
Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Required for the exosomal release of SDCBP, CD63 and syndecan.
Uniprot ID
P52849
Ensemble ID
ENST00000299641.8
HGNC ID
HGNC:7681

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CCK81 SNV: p.L189P .
HCT116 Deletion: p.A690LfsTer36 .
HCT15 SNV: p.W384C .
HEC1 SNV: p.L807V; p.R852W .
HEC1B SNV: p.L807V .
HELA SNV: p.V297I .
JURKAT Insertion: p.L580PfsTer7 .
KPL1 SNV: p.R502G .
MCF7 SNV: p.R502G .
MFE319 SNV: p.L189P .
OCILY3 SNV: p.V315E .
OVK18 Insertion: p.T848NfsTer8 .
SKMEL2 SNV: p.R258C .
SW403 SNV: p.L746M .
TCCSUP SNV: p.Q558H .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C306(0.66)  LDD1573  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0369  CL100 HEK-293T C306(0.66)  LDD1573  [1]
 LDCM0373  CL104 HEK-293T C306(0.85)  LDD1577  [1]
 LDCM0377  CL108 HEK-293T C306(0.90)  LDD1581  [1]
 LDCM0382  CL112 HEK-293T C306(0.80)  LDD1586  [1]
 LDCM0386  CL116 HEK-293T C306(0.93)  LDD1590  [1]
 LDCM0391  CL120 HEK-293T C306(0.74)  LDD1595  [1]
 LDCM0395  CL124 HEK-293T C306(0.72)  LDD1599  [1]
 LDCM0399  CL128 HEK-293T C306(0.84)  LDD1603  [1]
 LDCM0403  CL16 HEK-293T C306(0.71)  LDD1607  [1]
 LDCM0416  CL28 HEK-293T C306(0.71)  LDD1620  [1]
 LDCM0429  CL4 HEK-293T C306(0.79)  LDD1633  [1]
 LDCM0430  CL40 HEK-293T C306(0.84)  LDD1634  [1]
 LDCM0443  CL52 HEK-293T C306(0.73)  LDD1646  [1]
 LDCM0456  CL64 HEK-293T C306(0.88)  LDD1659  [1]
 LDCM0469  CL76 HEK-293T C306(0.70)  LDD1672  [1]
 LDCM0482  CL88 HEK-293T C306(0.78)  LDD1685  [1]

References

1 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402