Details of the Target

General Information of Target

Target ID LDTP04482
Target Name Aldo-keto reductase family 1 member D1 (AKR1D1)
Gene Name AKR1D1
Gene ID 6718
Synonyms
SRD5B1; Aldo-keto reductase family 1 member D1; EC 1.3.1.3; 3-oxo-5-beta-steroid 4-dehydrogenase; Delta(4)-3-ketosteroid 5-beta-reductase; Delta(4)-3-oxosteroid 5-beta-reductase
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDLSAASHRIPLSDGNSIPIIGLGTYSEPKSTPKGACATSVKVAIDTGYRHIDGAYIYQN
EHEVGEAIREKIAEGKVRREDIFYCGKLWATNHVPEMVRPTLERTLRVLQLDYVDLYIIE
VPMAFKPGDEIYPRDENGKWLYHKSNLCATWEAMEACKDAGLVKSLGVSNFNRRQLELIL
NKPGLKHKPVSNQVECHPYFTQPKLLKFCQQHDIVITAYSPLGTSRNPIWVNVSSPPLLK
DALLNSLGKRYNKTAAQIVLRFNIQRGVVVIPKSFNLERIKENFQIFDFSLTEEEMKDIE
ALNKNVRFVELLMWRDHPEYPFHDEY
Target Bioclass
Enzyme
Family
Aldo/keto reductase family
Subcellular location
Cytoplasm
Function
Catalyzes the stereospecific NADPH-dependent reduction of the C4-C5 double bond of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure to yield an A/B cis-ring junction. This cis-configuration is crucial for bile acid biosynthesis and plays important roles in steroid metabolism. Capable of reducing a broad range of delta-(4)-3-ketosteroids from C18 (such as, 17beta-hydroxyestr-4-en-3-one) to C27 (such as, 7alpha-hydroxycholest-4-en-3-one).
Uniprot ID
P51857
Ensemble ID
ENST00000242375.8
HGNC ID
HGNC:388

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HGC27 SNV: p.R134T .
HT115 SNV: p.S220N .
IGR1 SNV: p.Q257K .
RL952 SNV: p.D2G .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
YN-1
 Probe Info  		100.00  LDD0444  [1]
YN-4
 Probe Info  		100.00  LDD0445  [1]
IPM
 Probe Info  		N.A.  LDD0241  [2]
PAL-AfBPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STS-1
 Probe Info  		N.A.  LDD0136  [3]
STS-2
 Probe Info  		N.A.  LDD0139  [3]

The Interaction Atlas With This Target

The Drug(s) Related To This Target

Approved
Click To Hide/Show 5 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Azelaic Acid Small molecular drug DB00548
Finasteride Small molecular drug DB01216
Hydrocortisone Small molecular drug DB00741
Lumateperone Small molecular drug DB06077
Norethisterone Small molecular drug DB00717
Investigative
Click To Hide/Show 2 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
320-pregnanedione Small molecular drug DB07557
5beta-dihydrotestosterone . DB07447

References

1 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
2 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
3 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.