Details of the Target

General Information of Target

Target ID LDTP04208
Target Name Aminomethyltransferase, mitochondrial (AMT)
Gene Name AMT
Gene ID 275
Synonyms
GCST; Aminomethyltransferase, mitochondrial; EC 2.1.2.10; Glycine cleavage system T protein; GCVT
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MQRAVSVVARLGFRLQAFPPALCRPLSCAQEVLRRTPLYDFHLAHGGKMVAFAGWSLPVQ
YRDSHTDSHLHTRQHCSLFDVSHMLQTKILGSDRVKLMESLVVGDIAELRPNQGTLSLFT
NEAGGILDDLIVTNTSEGHLYVVSNAGCWEKDLALMQDKVRELQNQGRDVGLEVLDNALL
ALQGPTAAQVLQAGVADDLRKLPFMTSAVMEVFGVSGCRVTRCGYTGEDGVEISVPVAGA
VHLATAILKNPEVKLAGLAARDSLRLEAGLCLYGNDIDEHTTPVEGSLSWTLGKRRRAAM
DFPGAKVIVPQLKGRVQRRRVGLMCEGAPMRAHSPILNMEGTKIGTVTSGCPSPSLKKNV
AMGYVPCEYSRPGTMLLVEVRRKQQMAVVSKMPFVPTNYYTLK
Target Bioclass
Enzyme
Family
GcvT family
Subcellular location
Mitochondrion
Function The glycine cleavage system catalyzes the degradation of glycine.
Uniprot ID
P48728
Ensemble ID
ENST00000273588.9
HGNC ID
HGNC:473

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
COLO678 SNV: p.T221N .
EFO27 Deletion: p.A328PfsTer10 .
HEPG2 SNV: p.V81A .
MFE319 SNV: p.A328G .
MOLT4 SNV: p.R14H .
SISO SNV: p.R261K .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IPM
 Probe Info  		C367(0.00); C351(0.00)  LDD0241  [1]
DBIA
 Probe Info  		C351(1.94); C325(1.84)  LDD3424  [2]
W1
 Probe Info  		C367(0.80)  LDD0239  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 Hep 3B2.1-7 C351(1.13); C325(1.21)  LDD2353  [2]
 LDCM0023  KB03 Hep 3B2.1-7 C351(1.13); C325(1.14)  LDD2770  [2]
 LDCM0024  KB05 SK-HEP1 C351(1.94); C325(1.84)  LDD3424  [2]
 LDCM0112  W16 Hep-G2 C367(0.80)  LDD0239  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein VAC14 homolog (VAC14) VAC14 family Q08AM6
PDZ and LIM domain protein 4 (PDLIM4) . P50479

The Drug(s) Related To This Target

Approved
Click To Hide/Show 2 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Glycine Small molecular drug DB00145
Nadh Small molecular drug DB00157
Investigative
Click To Hide/Show 2 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Tetrahydrofolic Acid Small molecular drug DB00116
5-methyltetrahydrofolic Acid . DB04789

References

1 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840