Details of the Target

General Information of Target

Target ID LDTP03977
Target Name Rho GTPase-activating protein 25 (ARHGAP25)
Gene Name ARHGAP25
Gene ID 9938
Synonyms
KIAA0053; Rho GTPase-activating protein 25; Rho-type GTPase-activating protein 25
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSLKLPRNWDFNLKVEAAKIARSRSVMTGEQMAAFHPSSTPNPLERPIKMGWLKKQRSIV
KNWQQRYFVLRAQQLYYYKDEEDTKPQGCMYLPGCTIKEIATNPEEAGKFVFEIIPASWD
QNRMGQDSYVLMASSQAEMEEWVKFLRRVAGTPCGVFGQRLDETVAYEQKFGPHLVPILV
EKCAEFILEHGRNEEGIFRLPGQDNLVKQLRDAFDAGERPSFDRDTDVHTVASLLKLYLR
DLPEPVVPWSQYEGFLLCGQLTNADEAKAQQELMKQLSILPRDNYSLLSYICRFLHEIQL
NCAVNKMSVDNLATVIGVNLIRSKVEDPAVIMRGTPQIQRVMTMMIRDHEVLFPKSKDIP
LSPPAQKNDPKKAPVARSSVGWDATEDLRISRTDSFSSMTSDSDTTSPTGQQPSDAFPED
SSKVPREKPGDWKMQSRKRTQTLPNRKCFLTSAFQGANSSKMEIFKNEFWSPSSEAKAGE
GHRRTMSQDLRQLSDSQRTSTYDNVPSLPGSPGEEASALSSQACDSKGDTLASPNSETGP
GKKNSGEEEIDSLQRMVQELRKEIETQKQMYEEQIKNLEKENYDVWAKVVRLNEELEKEK
KKSAALEISLRNMERSREDVEKRNKALEEEVKEFVKSMKEPKTEA
Target Bioclass
Other
Function GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.
Uniprot ID
P42331
Ensemble ID
ENST00000409030.7
HGNC ID
HGNC:28951

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C184(1.95)  LDD3333  [1]
4-Iodoacetamidophenylacetylene
 Probe Info  		C183(0.00); C448(0.00); C292(0.00); C302(0.00)  LDD0038  [2]
IA-alkyne
 Probe Info  		C183(0.00); C258(0.00); C292(0.00); C302(0.00)  LDD0036  [2]
Lodoacetamide azide
 Probe Info  		C183(0.00); C292(0.00); C448(0.00); C258(0.00)  LDD0037  [2]
Compound 10
 Probe Info  		N.A.  LDD2216  [3]
Compound 11
 Probe Info  		N.A.  LDD2213  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0625  F8 Ramos C302(0.65); C448(1.17)  LDD2187  [4]
 LDCM0572  Fragment10 Ramos C302(0.30); C448(5.40)  LDD2189  [4]
 LDCM0573  Fragment11 Ramos C302(1.43); C448(1.04)  LDD2190  [4]
 LDCM0574  Fragment12 Ramos C302(0.48); C448(1.95)  LDD2191  [4]
 LDCM0575  Fragment13 Ramos C302(1.03); C448(0.82)  LDD2192  [4]
 LDCM0576  Fragment14 Ramos C302(0.75); C448(1.38)  LDD2193  [4]
 LDCM0579  Fragment20 Ramos C302(0.39)  LDD2194  [4]
 LDCM0580  Fragment21 Ramos C302(0.48); C448(0.91)  LDD2195  [4]
 LDCM0582  Fragment23 Ramos C302(0.16); C448(0.69)  LDD2196  [4]
 LDCM0578  Fragment27 Ramos C302(0.49); C448(0.93)  LDD2197  [4]
 LDCM0586  Fragment28 Ramos C448(1.04)  LDD2198  [4]
 LDCM0588  Fragment30 Ramos C302(0.34); C448(1.07)  LDD2199  [4]
 LDCM0589  Fragment31 Ramos C448(0.97)  LDD2200  [4]
 LDCM0590  Fragment32 Ramos C302(0.40); C448(0.94)  LDD2201  [4]
 LDCM0468  Fragment33 Ramos C302(0.65); C448(1.37)  LDD2202  [4]
 LDCM0596  Fragment38 Ramos C302(0.29); C448(0.77)  LDD2203  [4]
 LDCM0566  Fragment4 Ramos C302(0.75); C448(0.79)  LDD2184  [4]
 LDCM0610  Fragment52 Ramos C302(0.41); C448(1.03)  LDD2204  [4]
 LDCM0614  Fragment56 Ramos C302(0.47); C448(0.90)  LDD2205  [4]
 LDCM0569  Fragment7 Ramos C302(0.58); C448(0.75)  LDD2186  [4]
 LDCM0571  Fragment9 Ramos C302(0.33)  LDD2188  [4]
 LDCM0022  KB02 T cell C448(11.76)  LDD1703  [5]
 LDCM0023  KB03 Ramos C302(0.61); C448(1.34)  LDD2183  [4]
 LDCM0024  KB05 MOLM-13 C184(1.95)  LDD3333  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Voltage-dependent anion-selective channel protein 2 (VDAC2) Eukaryotic mitochondrial porin family P45880
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
T-complex protein 1 subunit zeta (CCT6A) TCP-1 chaperonin family P40227
Cadherin-related family member 3 (CDHR3) . Q6ZTQ4
Melanoma-associated antigen B6 (MAGEB6) . Q8N7X4

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
3 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
4 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
5 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.