Details of the Target

General Information of Target

Target ID LDTP03531
Target Name M-phase inducer phosphatase 1 (CDC25A)
Gene Name CDC25A
Gene ID 993
Synonyms
M-phase inducer phosphatase 1; EC 3.1.3.48; Dual specificity phosphatase Cdc25A
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MELGPEPPHRRRLLFACSPPPASQPVVKALFGASAAGGLSPVTNLTVTMDQLQGLGSDYE
QPLEVKNNSNLQRMGSSESTDSGFCLDSPGPLDSKENLENPMRRIHSLPQKLLGCSPALK
RSHSDSLDHDIFQLIDPDENKENEAFEFKKPVRPVSRGCLHSHGLQEGKDLFTQRQNSAP
ARMLSSNERDSSEPGNFIPLFTPQSPVTATLSDEDDGFVDLLDGENLKNEEETPSCMASL
WTAPLVMRTTNLDNRCKLFDSPSLCSSSTRSVLKRPERSQEESPPGSTKRRKSMSGASPK
ESTNPEKAHETLHQSLSLASSPKGTIENILDNDPRDLIGDFSKGYLFHTVAGKHQDLKYI
SPEIMASVLNGKFANLIKEFVIIDCRYPYEYEGGHIKGAVNLHMEEEVEDFLLKKPIVPT
DGKRVIVVFHCEFSSERGPRMCRYVRERDRLGNEYPKLHYPELYVLKGGYKEFFMKCQSY
CEPPSYRPMHHEDFKEDLKKFRTKSRTWAGEKSKREMYSRLKKL
Target Type
Literature-reported
Target Bioclass
Enzyme
Family
MPI phosphatase family
Function
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin-E, in vitro.
TTD ID
T30823
Uniprot ID
P30304
DrugMap ID
TTLZS4Q
Ensemble ID
ENST00000302506.8
HGNC ID
HGNC:1725
ChEMBL ID
CHEMBL3775

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 SNV: p.R446Ter .
AN3CA SNV: p.L451M .
HT SNV: p.R11H .
MOLT4 SNV: p.R443W .
OAW28 SNV: p.T208A .
RL952 SNV: p.A319V .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C159(1.06); C256(1.06)  LDD1513  [1]
IPM
 Probe Info  		C265(0.00); C159(0.00)  LDD2156  [2]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STS-1
 Probe Info  		N.A.  LDD0137  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0270  AC15 HEK-293T C159(1.06); C256(1.06)  LDD1513  [1]
 LDCM0283  AC23 HEK-293T C159(1.01); C256(1.05)  LDD1522  [1]
 LDCM0292  AC31 HEK-293T C159(0.95); C256(1.03)  LDD1531  [1]
 LDCM0300  AC39 HEK-293T C159(1.08); C256(1.06)  LDD1539  [1]
 LDCM0309  AC47 HEK-293T C159(1.04); C256(1.12)  LDD1548  [1]
 LDCM0318  AC55 HEK-293T C159(1.13); C256(1.05)  LDD1557  [1]
 LDCM0327  AC63 HEK-293T C159(1.08); C256(1.05)  LDD1566  [1]
 LDCM0334  AC7 HEK-293T C159(0.97); C256(1.04)  LDD1568  [1]
 LDCM0379  CL11 HEK-293T C159(1.02); C256(1.08)  LDD1583  [1]
 LDCM0411  CL23 HEK-293T C159(1.04); C256(1.23)  LDD1615  [1]
 LDCM0424  CL35 HEK-293T C159(1.00); C256(1.15)  LDD1628  [1]
 LDCM0437  CL47 HEK-293T C159(1.06); C256(1.07)  LDD1641  [1]
 LDCM0450  CL59 HEK-293T C159(1.04); C256(1.18)  LDD1653  [1]
 LDCM0464  CL71 HEK-293T C159(1.00); C256(1.23)  LDD1667  [1]
 LDCM0477  CL83 HEK-293T C159(1.09); C256(1.15)  LDD1680  [1]
 LDCM0490  CL95 HEK-293T C159(1.11); C256(1.06)  LDD1693  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
N-alpha-acetyltransferase 11 (NAA11) Acetyltransferase family Q9BSU3
RAF proto-oncogene serine/threonine-protein kinase (RAF1) TKL Ser/Thr protein kinase family P04049
Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
14-3-3 protein theta (YWHAQ) 14-3-3 family P27348
14-3-3 protein zeta/delta (YWHAZ) 14-3-3 family P63104
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
14-3-3 protein beta/alpha (YWHAB) 14-3-3 family P31946

The Drug(s) Related To This Target

Investigative
Click To Hide/Show 10 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
(E)-2-(1-decyl-2-oxoindolin-3-ylidene)Acetic Acid Small molecular drug D0GE3O
(Z)-2-(1-decyl-2-oxoindolin-3-ylidene)Acetic Acid Small molecular drug D0W1SP
1-dodecyl-1h-indole-2,3-dione Small molecular drug D00ANJ
2-(1-dodecyl-1h-indol-3-yl)Acetic Acid Small molecular drug D0CJ6P
3-isopropyl-4-(Phenylamino)Naphthalene-1,2-dione Small molecular drug D0X5WD
3-isopropyl-4-(Phenylthio)Naphthalene-1,2-dione Small molecular drug D08GWQ
3-isopropyl-4-phenylnaphthalene-1,2-dione Small molecular drug D07JRA
3-isopropylnaphthalene-1,2-dione Small molecular drug D00SAA
4-(P-toluidino)-3-isopropylnaphthalene-1,2-dione Small molecular drug D0N7YT
Nsc-95397 Small molecular drug D0J1KH
Discontinued
Click To Hide/Show 2 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Ro 20-1724 Small molecular drug D07FHZ
Mx-7065 . D06REJ

References

1 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
2 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
3 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.