Details of the Target

General Information of Target

Target ID LDTP03154
Target Name Alpha-(1,3)-fucosyltransferase 4 (FUT4)
Gene Name FUT4
Gene ID 2526
Synonyms
ELFT; FCT3A; Alpha-(1,3)-fucosyltransferase 4; 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase; EC 2.4.1.152; ELAM-1 ligand fucosyltransferase; Fucosyltransferase 4; Fucosyltransferase IV; Fuc-TIV; FucT-IV; Galactoside 3-L-fucosyltransferase
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MRRLWGAARKPSGAGWEKEWAEAPQEAPGAWSGRLGPGRSGRKGRAVPGWASWPAHLALA
ARPARHLGGAGQGPRPLHSGTAPFHSRASGERQRRLEPQLQHESRCRSSTPADAWRAEAA
LPVRAMGAPWGSPTAAAGGRRGWRRGRGLPWTVCVLAAAGLTCTALITYACWGQLPPLPW
ASPTPSRPVGVLLWWEPFGGRDSAPRPPPDCRLRFNISGCRLLTDRASYGEAQAVLFHHR
DLVKGPPDWPPPWGIQAHTAEEVDLRVLDYEEAAAAAEALATSSPRPPGQRWVWMNFESP
SHSPGLRSLASNLFNWTLSYRADSDVFVPYGYLYPRSHPGDPPSGLAPPLSRKQGLVAWV
VSHWDERQARVRYYHQLSQHVTVDVFGRGGPGQPVPEIGLLHTVARYKFYLAFENSQHLD
YITEKLWRNALLAGAVPVVLGPDRANYERFVPRGAFIHVDDFPSASSLASYLLFLDRNPA
VYRRYFHWRRSYAVHITSFWDEPWCRVCQAVQRAGDRPKSIRNLASWFER
Target Type
Successful
Target Bioclass
Enzyme
Family
Glycosyltransferase 10 family
Subcellular location
Golgi apparatus, Golgi stack membrane
Function
[Isoform Short]: Catalyzes alpha(1->3) linkage of fucosyl moiety transferred from GDP-beta-L-fucose to N-acetyl glucosamine (GlcNAc) within type 2 lactosamine (LacNAc, Gal-beta(1->4)GlcNAc) glycan attached to N- or O-linked glycoproteins. Robustly fucosylates nonsialylated distal LacNAc unit of the polylactosamine chain to form Lewis X antigen (CD15), a glycan determinant known to mediate important cellular functions in development and immunity. Fucosylates with lower efficiency sialylated LacNAc acceptors to form sialyl Lewis X and 6-sulfo sialyl Lewis X determinants that serve as recognition epitopes for C-type lectins. Together with FUT7 contributes to SELE, SELL and SELP selectin ligand biosynthesis and selectin-dependent lymphocyte homing, leukocyte migration and blood leukocyte homeostasis. In a cell type specific manner, may also fucosylate the internal LacNAc unit of the polylactosamine chain to form VIM-2 antigen that serves as recognition epitope for SELE.; [Isoform Long]: Does not generate Lewis X antigens.
TTD ID
T51146
Uniprot ID
P22083
DrugMap ID
TTNV1KZ
Ensemble ID
ENST00000358752.4
HGNC ID
HGNC:4015
ChEMBL ID
CHEMBL4996

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
EGI1 SNV: p.A60V .
HSC4 SNV: p.R307L .
NCIH1048 SNV: p.V459M .
NCIH1155 SNV: p.R307Ter .
RVH421 Substitution: p.R145Ter .
SKNSH SNV: p.W172L; p.P247H .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C508(1.67)  LDD3333  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0369  CL100 HEK-293T C211(1.11)  LDD1573  [2]
 LDCM0373  CL104 HEK-293T C211(1.12)  LDD1577  [2]
 LDCM0377  CL108 HEK-293T C211(1.06)  LDD1581  [2]
 LDCM0382  CL112 HEK-293T C211(1.12)  LDD1586  [2]
 LDCM0386  CL116 HEK-293T C211(0.97)  LDD1590  [2]
 LDCM0391  CL120 HEK-293T C211(1.01)  LDD1595  [2]
 LDCM0395  CL124 HEK-293T C211(1.06)  LDD1599  [2]
 LDCM0399  CL128 HEK-293T C211(1.12)  LDD1603  [2]
 LDCM0403  CL16 HEK-293T C211(1.15)  LDD1607  [2]
 LDCM0416  CL28 HEK-293T C211(1.02)  LDD1620  [2]
 LDCM0429  CL4 HEK-293T C211(0.94)  LDD1633  [2]
 LDCM0430  CL40 HEK-293T C211(1.18)  LDD1634  [2]
 LDCM0443  CL52 HEK-293T C211(1.14)  LDD1646  [2]
 LDCM0456  CL64 HEK-293T C211(1.04)  LDD1659  [2]
 LDCM0469  CL76 HEK-293T C211(1.17)  LDD1672  [2]
 LDCM0482  CL88 HEK-293T C211(1.16)  LDD1685  [2]
 LDCM0022  KB02 CMK C508(1.67)  LDD2302  [1]
 LDCM0023  KB03 CMK C508(2.31)  LDD2719  [1]
 LDCM0024  KB05 MOLM-13 C508(1.67)  LDD3333  [1]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402