Details of the Target

General Information of Target

Target ID LDTP03093
Target Name N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase (AGA)
Gene Name AGA
Gene ID 175
Synonyms
N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase; EC 3.5.1.26; Aspartylglucosaminidase; Glycosylasparaginase; N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase) [Cleaved into: Glycosylasparaginase alpha chain; Glycosylasparaginase beta chain]
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MARKSNLPVLLVPFLLCQALVRCSSPLPLVVNTWPFKNATEAAWRALASGGSALDAVESG
CAMCEREQCDGSVGFGGSPDELGETTLDAMIMDGTTMDVGAVGDLRRIKNAIGVARKVLE
HTTHTLLVGESATTFAQSMGFINEDLSTTASQALHSDWLARNCQPNYWRNVIPDPSKYCG
PYKPPGILKQDIPIHKETEDDRGHDTIGMVVIHKTGHIAAGTSTNGIKFKIHGRVGDSPI
PGAGAYADDTAGAAAATGNGDILMRFLPSYQAVEYMRRGEDPTIACQKVISRIQKHFPEF
FGAVICANVTGSYGAACNKLSTFTQFSFMVYNSEKNQPTEEKVDCI
Target Bioclass
Enzyme
Family
Ntn-hydrolase family
Subcellular location
Lysosome
Function Cleaves the GlcNAc-Asn bond which joins oligosaccharides to the peptide of asparagine-linked glycoproteins.
Uniprot ID
P20933
Ensemble ID
ENST00000264595.7
HGNC ID
HGNC:318

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C179(4.57)  LDD3428  [1]
IA-alkyne
 Probe Info  		N.A.  LDD0177  [2]
NAIA_4
 Probe Info  		N.A.  LDD2226  [3]
NAIA_5
 Probe Info  		N.A.  LDD2223  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0369  CL100 HEK-293T C179(1.06)  LDD1573  [4]
 LDCM0373  CL104 HEK-293T C179(1.14)  LDD1577  [4]
 LDCM0377  CL108 HEK-293T C179(0.98)  LDD1581  [4]
 LDCM0382  CL112 HEK-293T C179(0.99)  LDD1586  [4]
 LDCM0386  CL116 HEK-293T C179(1.06)  LDD1590  [4]
 LDCM0391  CL120 HEK-293T C179(1.20)  LDD1595  [4]
 LDCM0395  CL124 HEK-293T C179(0.98)  LDD1599  [4]
 LDCM0399  CL128 HEK-293T C179(1.08)  LDD1603  [4]
 LDCM0403  CL16 HEK-293T C179(0.98)  LDD1607  [4]
 LDCM0416  CL28 HEK-293T C179(1.01)  LDD1620  [4]
 LDCM0429  CL4 HEK-293T C179(1.18)  LDD1633  [4]
 LDCM0430  CL40 HEK-293T C179(0.91)  LDD1634  [4]
 LDCM0443  CL52 HEK-293T C179(1.02)  LDD1646  [4]
 LDCM0456  CL64 HEK-293T C179(1.22)  LDD1659  [4]
 LDCM0469  CL76 HEK-293T C179(0.95)  LDD1672  [4]
 LDCM0482  CL88 HEK-293T C179(1.19)  LDD1685  [4]
 LDCM0022  KB02 G-361 C179(3.01)  LDD2329  [1]
 LDCM0023  KB03 G-361 C179(2.82)  LDD2746  [1]
 LDCM0024  KB05 SK-MEL3 C179(4.57)  LDD3428  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Wolframin (WFS1) . O76024
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Neurofilament light polypeptide (NEFL) Intermediate filament family P07196
Keratin-associated protein 1-3 (KRTAP1-3) KRTAP type 1 family Q8IUG1

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
3 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
4 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402