Details of the Target

General Information of Target

Target ID LDTP03042
Target Name B-cell receptor CD22 (CD22)
Gene Name CD22
Gene ID 933
Synonyms
SIGLEC2; B-cell receptor CD22; B-lymphocyte cell adhesion molecule; BL-CAM; Sialic acid-binding Ig-like lectin 2; Siglec-2; T-cell surface antigen Leu-14; CD antigen CD22
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MHLLGPWLLLLVLEYLAFSDSSKWVFEHPETLYAWEGACVWIPCTYRALDGDLESFILFH
NPEYNKNTSKFDGTRLYESTKDGKVPSEQKRVQFLGDKNKNCTLSIHPVHLNDSGQLGLR
MESKTEKWMERIHLNVSERPFPPHIQLPPEIQESQEVTLTCLLNFSCYGYPIQLQWLLEG
VPMRQAAVTSTSLTIKSVFTRSELKFSPQWSHHGKIVTCQLQDADGKFLSNDTVQLNVKH
TPKLEIKVTPSDAIVREGDSVTMTCEVSSSNPEYTTVSWLKDGTSLKKQNTFTLNLREVT
KDQSGKYCCQVSNDVGPGRSEEVFLQVQYAPEPSTVQILHSPAVEGSQVEFLCMSLANPL
PTNYTWYHNGKEMQGRTEEKVHIPKILPWHAGTYSCVAENILGTGQRGPGAELDVQYPPK
KVTTVIQNPMPIREGDTVTLSCNYNSSNPSVTRYEWKPHGAWEEPSLGVLKIQNVGWDNT
TIACAACNSWCSWASPVALNVQYAPRDVRVRKIKPLSEIHSGNSVSLQCDFSSSHPKEVQ
FFWEKNGRLLGKESQLNFDSISPEDAGSYSCWVNNSIGQTASKAWTLEVLYAPRRLRVSM
SPGDQVMEGKSATLTCESDANPPVSHYTWFDWNNQSLPYHSQKLRLEPVKVQHSGAYWCQ
GTNSVGKGRSPLSTLTVYYSPETIGRRVAVGLGSCLAILILAICGLKLQRRWKRTQSQQG
LQENSSGQSFFVRNKKVRRAPLSEGPHSLGCYNPMMEDGISYTTLRFPEMNIPRTGDAES
SEMQRPPPDCDDTVTYSALHKRQVGDYENVIPDFPEDEGIHYSELIQFGVGERPQAQENV
DYVILKH
Target Type
Successful
Target Bioclass
Immunoglobulin
Family
Immunoglobulin superfamily, SIGLEC (sialic acid binding Ig-like lectin) family
Subcellular location
Cell membrane
Function
Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.
TTD ID
T37539
Uniprot ID
P20273
DrugMap ID
TTM6QSK
Ensemble ID
ENST00000085219.10
HGNC ID
HGNC:1643
ChEMBL ID
CHEMBL3218

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 SNV: p.D52E .
AGS SNV: p.L587R .
COLO792 SNV: p.T628I .
DU145 SNV: p.L587P .
IGROV1 SNV: p.D478E .
Ishikawa (Heraklio) 02 ER SNV: p.L550P .
MEWO SNV: p.R506Ter .
MFE319 Deletion: p.P142HfsTer18 .
MOLT4 SNV: p.V198A .
NCIH2172 SNV: p.C167W .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IA-alkyne
 Probe Info  		C790(1.30); C751(0.94)  LDD2182  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0625  F8 Ramos C790(1.60); C751(1.61)  LDD2187  [1]
 LDCM0572  Fragment10 Ramos C790(1.19)  LDD2189  [1]
 LDCM0573  Fragment11 Ramos C790(4.35); C751(1.08)  LDD2190  [1]
 LDCM0574  Fragment12 Ramos C790(0.83)  LDD2191  [1]
 LDCM0575  Fragment13 Ramos C790(0.96); C751(2.02)  LDD2192  [1]
 LDCM0576  Fragment14 Ramos C790(0.68); C751(1.62)  LDD2193  [1]
 LDCM0579  Fragment20 Ramos C790(0.54)  LDD2194  [1]
 LDCM0580  Fragment21 Ramos C790(1.02); C751(0.53)  LDD2195  [1]
 LDCM0582  Fragment23 Ramos C790(1.72); C751(1.16)  LDD2196  [1]
 LDCM0578  Fragment27 Ramos C790(1.28); C751(0.85)  LDD2197  [1]
 LDCM0586  Fragment28 Ramos C790(0.63); C751(0.79)  LDD2198  [1]
 LDCM0588  Fragment30 Ramos C790(1.31); C751(0.64)  LDD2199  [1]
 LDCM0589  Fragment31 Ramos C790(1.24); C751(0.88)  LDD2200  [1]
 LDCM0590  Fragment32 Ramos C790(1.45)  LDD2201  [1]
 LDCM0468  Fragment33 Ramos C790(1.24); C751(0.91)  LDD2202  [1]
 LDCM0596  Fragment38 Ramos C790(2.08)  LDD2203  [1]
 LDCM0566  Fragment4 Ramos C790(0.84); C751(0.95)  LDD2184  [1]
 LDCM0610  Fragment52 Ramos C790(0.92)  LDD2204  [1]
 LDCM0614  Fragment56 Ramos C790(1.02); C751(0.50)  LDD2205  [1]
 LDCM0569  Fragment7 Ramos C790(1.20); C751(1.09)  LDD2186  [1]
 LDCM0571  Fragment9 Ramos C790(0.71); C751(1.32)  LDD2188  [1]
 LDCM0022  KB02 Ramos C790(1.30); C751(0.94)  LDD2182  [1]
 LDCM0023  KB03 Ramos C790(1.08); C751(0.83)  LDD2183  [1]
 LDCM0024  KB05 Ramos C790(1.04); C751(2.08)  LDD2185  [1]

References

1 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578