Details of the Target

General Information of Target

Target ID LDTP02720
Target Name Hematopoietic lineage cell-specific protein (HCLS1)
Gene Name HCLS1
Gene ID 3059
Synonyms
HS1; Hematopoietic lineage cell-specific protein; Hematopoietic cell-specific LYN substrate 1; LckBP1; p75
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MWKSVVGHDVSVSVETQGDDWDTDPDFVNDISEKEQRWGAKTIEGSGRTEHINIHQLRNK
VSEEHDVLRKKEMESGPKASHGYGGRFGVERDRMDKSAVGHEYVAEVEKHSSQTDAAKGF
GGKYGVERDRADKSAVGFDYKGEVEKHTSQKDYSRGFGGRYGVEKDKWDKAALGYDYKGE
TEKHESQRDYAKGFGGQYGIQKDRVDKSAVGFNEMEAPTTAYKKTTPIEAASSGTRGLKA
KFESMAEEKRKREEEEKAQQVARRQQERKAVTKRSPEAPQPVIAMEEPAVPAPLPKKISS
EAWPPVGTPPSSESEPVRTSREHPVPLLPIRQTLPEDNEEPPALPPRTLEGLQVEEEPVY
EAEPEPEPEPEPEPENDYEDVEEMDRHEQEDEPEGDYEEVLEPEDSSFSSALAGSSGCPA
GAGAGAVALGISAVAVYDYQGEGSDELSFDPDDVITDIEMVDEGWWRGRCHGHFGLFPAN
YVKLLE
Target Bioclass
Other
Subcellular location
Membrane
Function
Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression.
Uniprot ID
P14317
Ensemble ID
ENST00000314583.8
HGNC ID
HGNC:4844

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A549 SNV: p.V482I .
HSC3 SNV: p.S154C .
HT SNV: p.S232P .
KYSE410 SNV: p.N213H .
MCC13 Substitution: p.G45M .
NALM6 Deletion: p.P293HfsTer25 .
SNGM SNV: p.E106G .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
TH211
 Probe Info  		Y161(20.00); Y83(20.00); Y177(18.17); Y175(10.19)  LDD0260  [1]
STPyne
 Probe Info  		K60(20.00)  LDD2218  [2]
DBIA
 Probe Info  		C470(1.46)  LDD3377  [3]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [4]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [4]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [4]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
VE-P
 Probe Info  		N.A.  LDD0396  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0625  F8 Ramos C470(3.13)  LDD2187  [6]
 LDCM0573  Fragment11 Ramos C470(0.38)  LDD2190  [6]
 LDCM0576  Fragment14 Ramos C470(0.71)  LDD2193  [6]
 LDCM0586  Fragment28 Ramos C470(1.20)  LDD2198  [6]
 LDCM0566  Fragment4 Ramos C470(0.73)  LDD2184  [6]
 LDCM0569  Fragment7 Ramos C470(0.54)  LDD2186  [6]
 LDCM0022  KB02 Ramos C470(0.34)  LDD2182  [6]
 LDCM0023  KB03 Ramos C470(0.55)  LDD2183  [6]
 LDCM0024  KB05 OPM-2 C470(1.46)  LDD3377  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
WAS/WASL-interacting protein family member 1 (WIPF1) Verprolin family O43516
SH2 domain-containing protein 4A (SH2D4A) . Q9H788
Telomeric repeat-binding factor 1 (TERF1) . P54274

References

1 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
2 Global profiling of lysine reactivity and ligandability in the human proteome. Nat Chem. 2017 Dec;9(12):1181-1190. doi: 10.1038/nchem.2826. Epub 2017 Jul 31.
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
5 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.
6 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578