Details of the Target

General Information of Target

Target ID LDTP01957
Target Name Fibronectin (FN1)
Gene Name FN1
Gene ID 2335
Synonyms
FN; Fibronectin; FN; Cold-insoluble globulin; CIG) [Cleaved into: Anastellin; Ugl-Y1; Ugl-Y2; Ugl-Y3]
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MLRGPGPGLLLLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPVAVSQSKPGCYDNGKHYQ
INQQWERTYLGNALVCTCYGGSRGFNCESKPEAEETCFDKYTGNTYRVGDTYERPKDSMI
WDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCK
PIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGSGRITCTSRNRCNDQDTRTSY
RIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQTTSSGSGPFTDVRAAVYQPQPHP
QPPPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPC
VLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQTRGGNSNGALC
HFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRI
GDQWDKQHDMGHMMRCTCVGNGRGEWTCIAYSQLRDQCIVDDITYNVNDTFHKRHEEGHM
LNCTCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQ
PLQTYPSSSGPVEVFITETPSQPNSHPIQWNAPQPSHISKYILRWRPKNSVGRWKEATIP
GHLNSYTIKGLKPGVVYEGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNTVTGETTPFSP
LVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDL
LPGRKYIVNVYQISEDGEQSLILSTSQTTAPDAPPDTTVDQVDDTSIVVRWSRPQAPITG
YRIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVVIQQETTG
TPRSDTVPSPRDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIPVNLPGEHGQRLPISRNT
FAEVTGLSPGVTYYFKVFAVSHGRESKPLTAQQTTKLDAPTNLQFVNETDSTVLVRWTPP
RAQITGYRLTVGLTRRGQPRQYNVGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPKATG
VFTTLQPGSSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVV
SGLTPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPPTNLHLEANPDTGVLTVSWERSTT
PDITGYRITTTPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEYNVSVYTVKDDKESVPIS
DTIIPEVPQLTDLSFVDITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIPIFEDFVDSSV
GYYTVTGLEPGIDYDISVITLINGGESAPTTLTQQTAVPPPTDLRFTNIGPDTMRVTWAP
PPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTP
LRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHS
RNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWD
APAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPA
SSKPISINYRTEIDKPSQMQVTDVQDNSISVKWLPSSSPVTGYRVTTTPKNGPGPTKTKT
AGPDQTEMTIEGLQPTVEYVVSVYAQNPSGESQPLVQTAVTNIDRPKGLAFTDVDVDSIK
IAWESPQGQVSRYRVTYSSPEDGIHELFPAPDGEEDTAELQGLRPGSEYTVSVVALHDDM
ESQPLIGTQSTAIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEIN
LAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETT
ITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDN
ARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVP
RPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTDELPQLVTLPHPNLHGPE
ILDVPSTVQKTPFVTHPGYDTGNGIQLPGTSGQQPSVGQQMIFEEHGFRRTTPPTTATPI
RHRPRPYPPNVGEEIQIGHIPREDVDYHLYPHGPGLNPNASTGQEALSQTTISWAPFQDT
SEYIISCHPVGTDEEPLQFRVPGTSTSATLTGLTRGATYNVIVEALKDQQRHKVREEVVT
VGNSVNEGLNQPTDDSCFDPYTVSHYAVGDEWERMSESGFKLLCQCLGFGSGHFRCDSSR
WCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQ
KEYLGAICSCTCFGGQRGWRCDNCRRPGGEPSPEGTTGQSYNQYSQRYHQRTNTNVNCPI
ECFMPLDVQADREDSRE
Target Type
Clinical trial
Target Bioclass
Other
Subcellular location
Secreted, extracellular space, extracellular matrix
Function
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation.; [Anastellin]: Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.; Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling.
TTD ID
T91696
Uniprot ID
P02751
DrugMap ID
TTPJ921
Ensemble ID
ENST00000323926.10
HGNC ID
HGNC:3778
ChEMBL ID
CHEMBL3810

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 10 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K2027(10.00); K2072(6.07); K248(7.69)  LDD0277  [1]
IPM
 Probe Info  		C2324(0.00); C2326(0.00); C231(0.00); C446(0.00)  LDD0241  [2]
OPA-S-S-alkyne
 Probe Info  		K1953(0.48); K2030(0.81); K1623(1.34); K1050(1.47)  LDD3494  [3]
DBIA
 Probe Info  		C135(1.89); C401(2.04)  LDD3311  [4]
BTD
 Probe Info  		C141(1.08)  LDD2089  [5]
AHL-Pu-1
 Probe Info  		C141(3.72)  LDD0170  [6]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [7]
Acrolein
 Probe Info  		H950(0.00); H58(0.00); H2394(0.00)  LDD0217  [8]
Methacrolein
 Probe Info  		C446(0.00); C374(0.00); C225(0.00)  LDD0218  [8]
NAIA_5
 Probe Info  		C141(0.00); C231(0.00); C2326(0.00); C2342(0.00)  LDD2223  [9]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C52(1.33); C135(0.96)  LDD2117  [5]
 LDCM0025  4SU-RNA DM93 C141(3.72)  LDD0170  [6]
 LDCM0545  Acetamide MDA-MB-231 C141(0.59)  LDD2138  [5]
 LDCM0498  BS-3668 MDA-MB-231 C141(0.79)  LDD2091  [5]
 LDCM0108  Chloroacetamide HeLa C135(0.00); H58(0.00); H2394(0.00)  LDD0222  [8]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C141(1.47); C135(1.32)  LDD1702  [5]
 LDCM0107  IAA HeLa H2394(0.00); H950(0.00); H58(0.00)  LDD0221  [8]
 LDCM0022  KB02 42-MG-BA C135(0.99); C401(1.53)  LDD2244  [4]
 LDCM0023  KB03 42-MG-BA C231(1.59); C401(1.46)  LDD2661  [4]
 LDCM0024  KB05 G361 C135(1.89); C401(2.04)  LDD3311  [4]
 LDCM0528  N-(4-bromophenyl)-2-cyano-N-phenylacetamide MDA-MB-231 C141(0.96)  LDD2121  [5]
 LDCM0109  NEM HeLa H950(0.00); H58(0.00); H2394(0.00)  LDD0223  [8]
 LDCM0496  Nucleophilic fragment 11a MDA-MB-231 C141(1.08)  LDD2089  [5]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C258(1.09)  LDD2099  [5]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C52(1.41); C135(0.81)  LDD2107  [5]
 LDCM0526  Nucleophilic fragment 26a MDA-MB-231 C135(1.00)  LDD2119  [5]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C52(1.03)  LDD2123  [5]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C52(1.82); C135(0.94)  LDD2125  [5]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C52(1.08)  LDD2127  [5]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C52(0.87); C135(0.42)  LDD2137  [5]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C52(1.33)  LDD2144  [5]
 LDCM0556  Nucleophilic fragment 8a MDA-MB-231 C141(0.56)  LDD2150  [5]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Lysyl oxidase homolog 2 (LOXL2) Lysyl oxidase family Q9Y4K0
Apolipoprotein(a) (LPA) Peptidase S1 family P08519
Tyrosine-protein kinase Fyn (FYN) Tyr protein kinase family P06241
Protein-glutamine gamma-glutamyltransferase 2 (TGM2) Transglutaminase family P21980
von Hippel-Lindau disease tumor suppressor (VHL) VHL family P40337
Tumor necrosis factor-inducible gene 6 protein (TNFAIP6) . P98066
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Stabilin-1 (STAB1) . Q9NY15
Cytokine and receptor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Uteroglobin (SCGB1A1) Secretoglobin family P11684
Other
Click To Hide/Show 11 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
CCN family member 2 (CCN2) CCN family P29279
Collagen alpha-1(I) chain (COL1A1) Fibrillar collagen family P02452
Collagen alpha-1(V) chain (COL5A1) Fibrillar collagen family P20908
Fibrillin-1 (FBN1) Fibrillin family P35555
Fibrillin-2 (FBN2) Fibrillin family P35556
Ficolin-3 (FCN3) Ficolin lectin family O75636
Plasma serine protease inhibitor (SERPINA5) Serpin family P05154
Decorin (DCN) Small leucine-rich proteoglycan (SLRP) family P07585
Thrombospondin-1 (THBS1) Thrombospondin family P07996
Fibronectin (FN1) . P02751
Ubiquilin-1 (UBQLN1) . Q9UMX0

The Drug(s) Related To This Target

Approved
Click To Hide/Show 5 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Ocriplasmin . D0Y7XG
Zinc . DB01593
Zinc Acetate . DB14487
Zinc Chloride . DB14533
Zinc Sulfate Unspecified Form . DB14548
Phase 2
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
L19-tnf-alpha Antibody D0GZ3A
Investigative
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Lanoteplase . DB06245

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
3 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
4 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
5 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
6 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
7 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
8 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
9 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264