Details of the Target

General Information of Target

Target ID LDTP01927
Target Name Collagen alpha-1(IV) chain (COL4A1)
Gene Name COL4A1
Gene ID 1282
Synonyms
Collagen alpha-1(IV) chain [Cleaved into: Arresten]
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGPRLSVWLLLLPAALLLHEEHSRAAAKGGCAGSGCGKCDCHGVKGQKGERGLPGLQGVI
GFPGMQGPEGPQGPPGQKGDTGEPGLPGTKGTRGPPGASGYPGNPGLPGIPGQDGPPGPP
GIPGCNGTKGERGPLGPPGLPGFAGNPGPPGLPGMKGDPGEILGHVPGMLLKGERGFPGI
PGTPGPPGLPGLQGPVGPPGFTGPPGPPGPPGPPGEKGQMGLSFQGPKGDKGDQGVSGPP
GVPGQAQVQEKGDFATKGEKGQKGEPGFQGMPGVGEKGEPGKPGPRGKPGKDGDKGEKGS
PGFPGEPGYPGLIGRQGPQGEKGEAGPPGPPGIVIGTGPLGEKGERGYPGTPGPRGEPGP
KGFPGLPGQPGPPGLPVPGQAGAPGFPGERGEKGDRGFPGTSLPGPSGRDGLPGPPGSPG
PPGQPGYTNGIVECQPGPPGDQGPPGIPGQPGFIGEIGEKGQKGESCLICDIDGYRGPPG
PQGPPGEIGFPGQPGAKGDRGLPGRDGVAGVPGPQGTPGLIGQPGAKGEPGEFYFDLRLK
GDKGDPGFPGQPGMPGRAGSPGRDGHPGLPGPKGSPGSVGLKGERGPPGGVGFPGSRGDT
GPPGPPGYGPAGPIGDKGQAGFPGGPGSPGLPGPKGEPGKIVPLPGPPGAEGLPGSPGFP
GPQGDRGFPGTPGRPGLPGEKGAVGQPGIGFPGPPGPKGVDGLPGDMGPPGTPGRPGFNG
LPGNPGVQGQKGEPGVGLPGLKGLPGLPGIPGTPGEKGSIGVPGVPGEHGAIGPPGLQGI
RGEPGPPGLPGSVGSPGVPGIGPPGARGPPGGQGPPGLSGPPGIKGEKGFPGFPGLDMPG
PKGDKGAQGLPGITGQSGLPGLPGQQGAPGIPGFPGSKGEMGVMGTPGQPGSPGPVGAPG
LPGEKGDHGFPGSSGPRGDPGLKGDKGDVGLPGKPGSMDKVDMGSMKGQKGDQGEKGQIG
PIGEKGSRGDPGTPGVPGKDGQAGQPGQPGPKGDPGISGTPGAPGLPGPKGSVGGMGLPG
TPGEKGVPGIPGPQGSPGLPGDKGAKGEKGQAGPPGIGIPGLRGEKGDQGIAGFPGSPGE
KGEKGSIGIPGMPGSPGLKGSPGSVGYPGSPGLPGEKGDKGLPGLDGIPGVKGEAGLPGT
PGPTGPAGQKGEPGSDGIPGSAGEKGEPGLPGRGFPGFPGAKGDKGSKGEVGFPGLAGSP
GIPGSKGEQGFMGPPGPQGQPGLPGSPGHATEGPKGDRGPQGQPGLPGLPGPMGPPGLPG
IDGVKGDKGNPGWPGAPGVPGPKGDPGFQGMPGIGGSPGITGSKGDMGPPGVPGFQGPKG
LPGLQGIKGDQGDQGVPGAKGLPGPPGPPGPYDIIKGEPGLPGPEGPPGLKGLQGLPGPK
GQQGVTGLVGIPGPPGIPGFDGAPGQKGEMGPAGPTGPRGFPGPPGPDGLPGSMGPPGTP
SVDHGFLVTRHSQTIDDPQCPSGTKILYHGYSLLYVQGNERAHGQDLGTAGSCLRKFSTM
PFLFCNINNVCNFASRNDYSYWLSTPEPMPMSMAPITGENIRPFISRCAVCEAPAMVMAV
HSQTIQIPPCPSGWSSLWIGYSFVMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRG
TCNYYANAYSFWLATIERSEMFKKPTPSTLKAGELRTHVSRCQVCMRRT
Target Bioclass
Other
Family
Type IV collagen family
Subcellular location
Secreted, extracellular space, extracellular matrix, basement membrane
Function
Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.; Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation.
Uniprot ID
P02462
Ensemble ID
ENST00000375820.10
HGNC ID
HGNC:2202
ChEMBL ID
CHEMBL2364188

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A2780 SNV: p.R1638K .
A375 SNV: p.P349S DBIA    Probe Info 
CCK81 Deletion: p.P588LfsTer214 .
COLO679 SNV: p.P1310S .
DU145 SNV: p.G160S .
HCC1187 SNV: p.T1449A .
HEC1 SNV: p.G118D; p.R807S .
HEC1B SNV: p.G118D; p.R807S .
HT SNV: p.L1447P .
IGR37 SNV: p.T671P .
IGROV1 Deletion: p.I641LfsTer161 .
LS180 SNV: p.G1121A .
LU65 SNV: p.P3A .
MDST8 SNV: p.P448S .
NCIH1793 SNV: p.A1611G .
NCIH2291 SNV: p.E433Ter .
OVK18 SNV: p.P1138H .
PC9 SNV: p.Q523H .
RL952 SNV: p.P851T .
RT112 SNV: p.M1410I .
SKMEL3 SNV: p.S1226F .
SKMEL5 SNV: p.E768K .
SKMM2 SNV: p.P198T .
SUPT1 SNV: p.Q1331R .
TE4 SNV: p.G966A .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
BTD
 Probe Info  		C1493(0.74); C1460(0.73)  LDD2123  [1]
DBIA
 Probe Info  		C31(0.85); C36(0.85)  LDD0078  [2]
IA-alkyne
 Probe Info  		C1493(0.00); C1460(0.00); C1616(0.00)  LDD0165  [3]
Acrolein
 Probe Info  		C1460(0.00); C1493(0.00)  LDD0217  [4]
Cinnamaldehyde
 Probe Info  		N.A.  LDD0220  [4]
Crotonaldehyde
 Probe Info  		H1483(0.00); C1493(0.00)  LDD0219  [4]
Methacrolein
 Probe Info  		C1460(0.00); C31(0.00); C1493(0.00)  LDD0218  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0281  AC21 HEK-293T C1616(1.03)  LDD1520  [5]
 LDCM0289  AC29 HEK-293T C1616(1.05)  LDD1528  [5]
 LDCM0298  AC37 HEK-293T C1616(1.06)  LDD1537  [5]
 LDCM0307  AC45 HEK-293T C1616(1.09)  LDD1546  [5]
 LDCM0312  AC5 HEK-293T C1616(1.03)  LDD1551  [5]
 LDCM0316  AC53 HEK-293T C1616(1.03)  LDD1555  [5]
 LDCM0325  AC61 HEK-293T C1616(1.08)  LDD1564  [5]
 LDCM0248  AKOS034007472 HEK-293T C1616(1.00)  LDD1511  [5]
 LDCM0020  ARS-1620 HCC44 C31(0.85); C36(0.85)  LDD0078  [2]
 LDCM0108  Chloroacetamide HeLa C1493(0.00); C1460(0.00)  LDD0222  [4]
 LDCM0367  CL1 HEK-293T C1616(1.10)  LDD1571  [5]
 LDCM0369  CL100 HEK-293T C31(1.03)  LDD1573  [5]
 LDCM0370  CL101 HEK-293T C1616(0.99)  LDD1574  [5]
 LDCM0373  CL104 HEK-293T C31(1.02)  LDD1577  [5]
 LDCM0374  CL105 HEK-293T C1616(0.92)  LDD1578  [5]
 LDCM0377  CL108 HEK-293T C31(0.92)  LDD1581  [5]
 LDCM0378  CL109 HEK-293T C1616(0.94)  LDD1582  [5]
 LDCM0382  CL112 HEK-293T C31(0.95)  LDD1586  [5]
 LDCM0383  CL113 HEK-293T C1616(1.01)  LDD1587  [5]
 LDCM0386  CL116 HEK-293T C31(0.99)  LDD1590  [5]
 LDCM0387  CL117 HEK-293T C1616(1.09)  LDD1591  [5]
 LDCM0391  CL120 HEK-293T C31(1.06)  LDD1595  [5]
 LDCM0392  CL121 HEK-293T C1616(1.04)  LDD1596  [5]
 LDCM0395  CL124 HEK-293T C31(0.97)  LDD1599  [5]
 LDCM0396  CL125 HEK-293T C1616(1.13)  LDD1600  [5]
 LDCM0399  CL128 HEK-293T C31(0.95)  LDD1603  [5]
 LDCM0400  CL13 HEK-293T C1616(0.93)  LDD1604  [5]
 LDCM0403  CL16 HEK-293T C31(1.12)  LDD1607  [5]
 LDCM0409  CL21 HEK-293T C1616(1.17)  LDD1613  [5]
 LDCM0413  CL25 HEK-293T C1616(1.15)  LDD1617  [5]
 LDCM0416  CL28 HEK-293T C31(0.96)  LDD1620  [5]
 LDCM0422  CL33 HEK-293T C1616(1.06)  LDD1626  [5]
 LDCM0426  CL37 HEK-293T C1616(1.13)  LDD1630  [5]
 LDCM0429  CL4 HEK-293T C31(1.06)  LDD1633  [5]
 LDCM0430  CL40 HEK-293T C31(1.00)  LDD1634  [5]
 LDCM0435  CL45 HEK-293T C1616(1.10)  LDD1639  [5]
 LDCM0439  CL49 HEK-293T C1616(0.99)  LDD1643  [5]
 LDCM0443  CL52 HEK-293T C31(1.03)  LDD1646  [5]
 LDCM0448  CL57 HEK-293T C1616(1.06)  LDD1651  [5]
 LDCM0453  CL61 HEK-293T C1616(1.01)  LDD1656  [5]
 LDCM0456  CL64 HEK-293T C31(1.07)  LDD1659  [5]
 LDCM0461  CL69 HEK-293T C1616(1.09)  LDD1664  [5]
 LDCM0466  CL73 HEK-293T C1616(1.11)  LDD1669  [5]
 LDCM0469  CL76 HEK-293T C31(0.96)  LDD1672  [5]
 LDCM0475  CL81 HEK-293T C1616(1.11)  LDD1678  [5]
 LDCM0479  CL85 HEK-293T C1616(1.32)  LDD1682  [5]
 LDCM0482  CL88 HEK-293T C31(0.95)  LDD1685  [5]
 LDCM0484  CL9 HEK-293T C1616(1.19)  LDD1687  [5]
 LDCM0488  CL93 HEK-293T C1616(1.14)  LDD1691  [5]
 LDCM0492  CL97 HEK-293T C1616(1.10)  LDD1695  [5]
 LDCM0107  IAA HeLa N.A.  LDD0221  [4]
 LDCM0022  KB02 A101D C1460(2.21)  LDD2250  [6]
 LDCM0023  KB03 A101D C1460(2.05)  LDD2667  [6]
 LDCM0024  KB05 SKMEL2 C1460(2.24)  LDD3322  [6]
 LDCM0109  NEM HeLa N.A.  LDD0223  [4]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C1493(0.74); C1460(0.73)  LDD2123  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Collagen alpha-2(IV) chain (COL4A2) Type IV collagen family P08572

References

1 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
2 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
3 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
4 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
6 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840