Details of the Target

General Information of Target

Target ID LDTP01527
Target Name Epsin-2 (EPN2)
Gene Name EPN2
Gene ID 22905
Synonyms
KIAA1065; Epsin-2; EPS-15-interacting protein 2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MTTSSIRRQMKNIVNNYSEAEIKVREATSNDPWGPSSSLMTEIADLTYNVVAFSEIMSMV
WKRLNDHGKNWRHVYKALTLLDYLIKTGSERVAQQCRENIFAIQTLKDFQYIDRDGKDQG
INVREKSKQLVALLKDEERLKAERAQALKTKERMAQVATGMGSNQITFGRGSSQPNLSTS
HSEQEYGKAGGSPASYHGSPEASLCPQHRTGAPLGQSEELQPLSQRHPFLPHLGLASRPN
GDWSQPCLTCDRAARATSPRVSSELEQARPQTSGEEELQLQLALAMSREVAEQEERLRRG
DDLRLQMALEESRRDTVKIPKKKEHGSLPQQTTLLDLMDALPSSGPAAQKAEPWGPSAST
NQTNPWGGPAAPASTSDPWPSFGTKPAASIDPWGVPTGATVQSVPKNSDPWAASQQPASS
AGKRASDAWGAVSTTKPVSVSGSFELFSNLNGTIKDDFSEFDNLRTSKKTAESVTSLPSQ
NNGTTSPDPFESQPLTVASSKPSSARKTPESFLGPNAALVNLDSLVTRPAPPAQSLNPFL
APGAPATSAPVNPFQVNQPQPLTLNQLRGSPVLGTSTSFGPGPGVESMAVASMTSAAPQP
ALGATGSSLTPLGPAMMNMVGSVGIPPSAAQATGTTNPFLL
Target Bioclass
Other
Family
Epsin family
Subcellular location
Cytoplasm
Function Plays a role in the formation of clathrin-coated invaginations and endocytosis.
Uniprot ID
O95208
Ensemble ID
ENST00000314728.10
HGNC ID
HGNC:18639

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
COLO792 SNV: p.S54L .
DU145 SNV: p.L177I .
HEC1 SNV: p.L612M .
HEC1B SNV: p.R238S .
KU1919 SNV: p.S38F .
NALM6 SNV: p.R252H .
NCIH1155 SNV: p.E90K .
REH Insertion: p.H181_S182insPTLSPSH .
SKMEL2 SNV: p.K151R .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C96(3.54)  LDD3325  [1]
BTD
 Probe Info  		C96(3.60)  LDD1700  [2]
IA-alkyne
 Probe Info  		N.A.  LDD0162  [3]
TFBX
 Probe Info  		N.A.  LDD0148  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C96(0.86)  LDD2117  [2]
 LDCM0558  2-Cyano-N-phenylacetamide MDA-MB-231 C96(1.41)  LDD2152  [2]
 LDCM0498  BS-3668 MDA-MB-231 C96(0.61)  LDD2091  [2]
 LDCM0022  KB02 A-549 C96(1.70)  LDD2260  [1]
 LDCM0023  KB03 A-549 C96(2.07)  LDD2677  [1]
 LDCM0024  KB05 UACC257 C96(3.54)  LDD3325  [1]
 LDCM0528  N-(4-bromophenyl)-2-cyano-N-phenylacetamide MDA-MB-231 C96(0.50)  LDD2121  [2]
 LDCM0496  Nucleophilic fragment 11a MDA-MB-231 C96(1.68)  LDD2089  [2]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C96(1.36)  LDD2093  [2]
 LDCM0504  Nucleophilic fragment 15a MDA-MB-231 C96(0.69)  LDD2097  [2]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C96(1.06)  LDD2099  [2]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C96(0.91)  LDD2107  [2]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C96(0.47)  LDD2109  [2]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C96(1.13)  LDD2111  [2]
 LDCM0522  Nucleophilic fragment 24a MDA-MB-231 C96(0.47)  LDD2115  [2]
 LDCM0525  Nucleophilic fragment 25b MDA-MB-231 C96(2.34)  LDD2118  [2]
 LDCM0526  Nucleophilic fragment 26a MDA-MB-231 C96(2.40)  LDD2119  [2]
 LDCM0529  Nucleophilic fragment 27b MDA-MB-231 C96(0.62)  LDD2122  [2]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C96(0.83)  LDD2123  [2]
 LDCM0531  Nucleophilic fragment 28b MDA-MB-231 C96(1.22)  LDD2124  [2]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C96(0.71)  LDD2125  [2]
 LDCM0533  Nucleophilic fragment 29b MDA-MB-231 C96(0.85)  LDD2126  [2]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C96(1.01)  LDD2127  [2]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C96(1.22)  LDD2129  [2]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C96(1.43)  LDD2135  [2]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C96(1.15)  LDD2136  [2]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C96(0.99)  LDD2137  [2]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C96(3.60)  LDD1700  [2]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C96(0.87)  LDD2140  [2]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C96(2.94)  LDD2144  [2]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C96(0.83)  LDD2146  [2]
 LDCM0556  Nucleophilic fragment 8a MDA-MB-231 C96(0.42)  LDD2150  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 11 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Arrestin domain-containing protein 1 (ARRDC1) Arrestin family Q8N5I2
Arrestin domain-containing protein 2 (ARRDC2) Arrestin family Q8TBH0
Fanconi anemia group M protein (FANCM) DEAD box helicase family Q8IYD8
Thimet oligopeptidase (THOP1) Peptidase M3 family P52888
5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2) CAMK Ser/Thr protein kinase family P54646
E3 ubiquitin-protein ligase RNF8 (RNF8) RNF8 family O76064
E3 ubiquitin-protein ligase TRIM23 (TRIM23) Arf family P36406
E3 ubiquitin-protein ligase Midline-1 (MID1) TRIM/RBCC family O15344
E3 ubiquitin-protein ligase TRIM62 (TRIM62) TRIM/RBCC family Q9BVG3
Tripartite motif-containing protein 26 (TRIM26) TRIM/RBCC family Q12899
Type 1 phosphatidylinositol 4,5-bisphosphate 4-phosphatase (PIP4P1) . Q86T03
Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Arrestin domain-containing protein 3 (ARRDC3) Arrestin family Q96B67
Protein lifeguard 2 (FAIM2) BI1 family Q9BWQ8
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Mothers against decapentaplegic homolog 1 (SMAD1) Dwarfin/SMAD family Q15797
Other
Click To Hide/Show 14 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ataxin-1 (ATXN1) ATXN1 family P54253
Cell death-inducing p53-target protein 1 (CDIP1) CDIP1/LITAF family Q9H305
Lipopolysaccharide-induced tumor necrosis factor-alpha factor (LITAF) CDIP1/LITAF family Q99732
Eukaryotic translation initiation factor 3 subunit E (EIF3E) EIF-3 subunit E family P60228
Alpha-taxilin (TXLNA) Taxilin family P40222
Polyubiquitin-C (UBC) Ubiquitin family P0CG48
Ubiquitin-ribosomal protein eL40 fusion protein (UBA52) Ubiquitin family; Eukaryotic ribosomal protein eL40 family P62987
DAZ-associated protein 2 (DAZAP2) . Q15038
Melanoma antigen recognized by T-cells 1 (MLANA) . Q16655
Pleckstrin homology domain-containing family B member 2 (PLEKHB2) . Q96CS7
Spartin (SPART) . Q8N0X7
WW domain binding protein 1-like (WBP1L) . Q9NX94
WW domain-binding protein 1 (WBP1) . Q96G27
WW domain-binding protein 2 (WBP2) . Q969T9

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
3 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
4 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255