Details of the Target

General Information of Target

Target ID LDTP00918
Target Name Protein phosphatase 1 regulatory subunit 12B (PPP1R12B)
Gene Name PPP1R12B
Gene ID 4660
Synonyms
MYPT2; Protein phosphatase 1 regulatory subunit 12B; Myosin phosphatase-targeting subunit 2; Myosin phosphatase target subunit 2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAELEHLGGKRAESARMRRAEQLRRWRGSLTEQEPAERRGAGRQPLTRRGSPRVRFEDGA
VFLAACSSGDTDEVRKLLARGADINTVNVDGLTALHQACIDENLDMVKFLVENRANVNQQ
DNEGWTPLHAAASCGYLNIAEYFINHGASVGIVNSEGEVPSDLAEEPAMKDLLLEQVKKQ
GVDLEQSRKEEEQQMLQDARQWLNSGKIEDVRQARSGATALHVAAAKGYSEVLRLLIQAG
YELNVQDYDGWTPLHAAAHWGVKEACSILAEALCDMDIRNKLGQTPFDVADEGLVEHLEL
LQKKQNVLRSEKETRNKLIESDLNSKIQSGFFKNKEKMLYEEETPKSQEMEEENKESSSS
SSEEEEGEDEASESETEKEADKKPEAFVNHSNSESKSSITEQIPAPAQNTFSASSARRFS
SGLFNKPEEPKDESPSSWRLGLRKTGSHNMLSEVANSREPIRDRGSSIYRSSSSPRISAL
LDNKDKERENKSYISSLAPRKLNSTSDIEEKENRESAVNLVRSGSYTRQLWRDEAKGNEI
PQTIAPSTYVSTYLKRTPHKSQADTTAEKTADNVSSSTPLCVITNRPLPSTANGVTATPV
LSITGTDSSVEAREKRRSYLTPVRDEEAESLRKARSRQARQTRRSTQGVTLTDLQEAERT
FSRSRAERQAQEQPREKPTDTEGLEGSPEKHEPSAVPATEAGEGQQPWGRSLDEEPICHR
LRCPAQPDKPTTPASPSTSRPSLYTSSHLLWTNRFSVPDSESSETTTNTTTAKEMDKNEN
EEADLDEQSSKRLSIRERRRPKERRRGTGINFWTKDEDETDGSEEVKETWHERLSRLESG
GSNPTTSDSYGDRASARARREAREARLATLTSRVEEDSNRDYKKLYESALTENQKLKTKL
QEAQLELADIKSKLEKVAQQKQEKTSDRSSVLEMEKRERRALERKMSEMEEEMKVLTELK
SDNQRLKDENGALIRVISKLSK
Target Bioclass
Other
Subcellular location
Cytoplasm, cytoskeleton
Function Regulates myosin phosphatase activity. Augments Ca(2+) sensitivity of the contractile apparatus.
Uniprot ID
O60237
Ensemble ID
ENST00000290419.9
HGNC ID
HGNC:7619

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AU565 SNV: p.E453Ter .
EFO27 SNV: p.A455V .
ICC8 SNV: p.L137V .
IGROV1 SNV: p.K945N .
MEWO SNV: p.P736S .
OVKATE SNV: p.V610L .
SW1116 SNV: p.L30R; p.E32V .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C779(2.04)  LDD3401  [1]
Acrolein
 Probe Info  		N.A.  LDD0229  [2]
IA-alkyne
 Probe Info  		C718(5.31)  LDD1706  [3]
N1
 Probe Info  		N.A.  LDD0245  [4]
IPM
 Probe Info  		N.A.  LDD0147  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C718(1.06)  LDD1508  [6]
 LDCM0226  AC11 HEK-293T C718(1.16)  LDD1509  [6]
 LDCM0270  AC15 HEK-293T C718(1.00)  LDD1513  [6]
 LDCM0277  AC18 HEK-293T C718(1.09)  LDD1516  [6]
 LDCM0278  AC19 HEK-293T C718(1.19)  LDD1517  [6]
 LDCM0279  AC2 HEK-293T C718(1.08)  LDD1518  [6]
 LDCM0281  AC21 HEK-293T C718(0.98)  LDD1520  [6]
 LDCM0283  AC23 HEK-293T C718(0.87)  LDD1522  [6]
 LDCM0286  AC26 HEK-293T C718(1.03)  LDD1525  [6]
 LDCM0287  AC27 HEK-293T C718(1.06)  LDD1526  [6]
 LDCM0289  AC29 HEK-293T C718(1.01)  LDD1528  [6]
 LDCM0290  AC3 HEK-293T C718(1.09)  LDD1529  [6]
 LDCM0292  AC31 HEK-293T C718(0.97)  LDD1531  [6]
 LDCM0295  AC34 HEK-293T C718(1.10)  LDD1534  [6]
 LDCM0296  AC35 HEK-293T C718(1.05)  LDD1535  [6]
 LDCM0298  AC37 HEK-293T C718(0.90)  LDD1537  [6]
 LDCM0300  AC39 HEK-293T C718(1.01)  LDD1539  [6]
 LDCM0304  AC42 HEK-293T C718(0.99)  LDD1543  [6]
 LDCM0305  AC43 HEK-293T C718(1.13)  LDD1544  [6]
 LDCM0307  AC45 HEK-293T C718(1.02)  LDD1546  [6]
 LDCM0309  AC47 HEK-293T C718(1.00)  LDD1548  [6]
 LDCM0312  AC5 HEK-293T C718(1.06)  LDD1551  [6]
 LDCM0313  AC50 HEK-293T C718(1.06)  LDD1552  [6]
 LDCM0314  AC51 HEK-293T C718(1.06)  LDD1553  [6]
 LDCM0316  AC53 HEK-293T C718(0.99)  LDD1555  [6]
 LDCM0318  AC55 HEK-293T C718(0.91)  LDD1557  [6]
 LDCM0321  AC58 HEK-293T C718(1.10)  LDD1560  [6]
 LDCM0322  AC59 HEK-293T C718(1.10)  LDD1561  [6]
 LDCM0325  AC61 HEK-293T C718(1.13)  LDD1564  [6]
 LDCM0327  AC63 HEK-293T C718(1.01)  LDD1566  [6]
 LDCM0334  AC7 HEK-293T C718(1.03)  LDD1568  [6]
 LDCM0248  AKOS034007472 HEK-293T C718(0.97)  LDD1511  [6]
 LDCM0367  CL1 HEK-293T C718(1.17)  LDD1571  [6]
 LDCM0370  CL101 HEK-293T C718(1.11)  LDD1574  [6]
 LDCM0371  CL102 HEK-293T C718(1.03)  LDD1575  [6]
 LDCM0374  CL105 HEK-293T C718(1.12)  LDD1578  [6]
 LDCM0375  CL106 HEK-293T C718(1.02)  LDD1579  [6]
 LDCM0378  CL109 HEK-293T C718(1.04)  LDD1582  [6]
 LDCM0379  CL11 HEK-293T C718(0.96)  LDD1583  [6]
 LDCM0380  CL110 HEK-293T C718(0.98)  LDD1584  [6]
 LDCM0383  CL113 HEK-293T C718(1.08)  LDD1587  [6]
 LDCM0384  CL114 HEK-293T C718(1.03)  LDD1588  [6]
 LDCM0387  CL117 HEK-293T C718(1.06)  LDD1591  [6]
 LDCM0388  CL118 HEK-293T C718(1.12)  LDD1592  [6]
 LDCM0392  CL121 HEK-293T C718(1.05)  LDD1596  [6]
 LDCM0393  CL122 HEK-293T C718(1.05)  LDD1597  [6]
 LDCM0396  CL125 HEK-293T C718(1.04)  LDD1600  [6]
 LDCM0397  CL126 HEK-293T C718(1.05)  LDD1601  [6]
 LDCM0400  CL13 HEK-293T C718(1.20)  LDD1604  [6]
 LDCM0401  CL14 HEK-293T C718(1.03)  LDD1605  [6]
 LDCM0405  CL18 HEK-293T C718(1.09)  LDD1609  [6]
 LDCM0406  CL19 HEK-293T C718(1.14)  LDD1610  [6]
 LDCM0407  CL2 HEK-293T C718(0.99)  LDD1611  [6]
 LDCM0409  CL21 HEK-293T C718(0.91)  LDD1613  [6]
 LDCM0411  CL23 HEK-293T C718(0.85)  LDD1615  [6]
 LDCM0413  CL25 HEK-293T C718(1.05)  LDD1617  [6]
 LDCM0414  CL26 HEK-293T C718(1.03)  LDD1618  [6]
 LDCM0419  CL30 HEK-293T C718(0.97)  LDD1623  [6]
 LDCM0420  CL31 HEK-293T C718(0.99)  LDD1624  [6]
 LDCM0422  CL33 HEK-293T C718(0.90)  LDD1626  [6]
 LDCM0424  CL35 HEK-293T C718(0.86)  LDD1628  [6]
 LDCM0426  CL37 HEK-293T C718(0.99)  LDD1630  [6]
 LDCM0432  CL42 HEK-293T C718(1.07)  LDD1636  [6]
 LDCM0433  CL43 HEK-293T C718(1.11)  LDD1637  [6]
 LDCM0435  CL45 HEK-293T C718(0.96)  LDD1639  [6]
 LDCM0437  CL47 HEK-293T C718(0.80)  LDD1641  [6]
 LDCM0439  CL49 HEK-293T C718(1.07)  LDD1643  [6]
 LDCM0441  CL50 HEK-293T C718(0.97)  LDD1645  [6]
 LDCM0445  CL54 HEK-293T C718(0.97)  LDD1648  [6]
 LDCM0446  CL55 HEK-293T C718(1.04)  LDD1649  [6]
 LDCM0448  CL57 HEK-293T C718(1.04)  LDD1651  [6]
 LDCM0450  CL59 HEK-293T C718(0.84)  LDD1653  [6]
 LDCM0451  CL6 HEK-293T C718(1.20)  LDD1654  [6]
 LDCM0453  CL61 HEK-293T C718(1.01)  LDD1656  [6]
 LDCM0454  CL62 HEK-293T C718(1.06)  LDD1657  [6]
 LDCM0458  CL66 HEK-293T C718(1.03)  LDD1661  [6]
 LDCM0459  CL67 HEK-293T C718(1.21)  LDD1662  [6]
 LDCM0461  CL69 HEK-293T C718(0.96)  LDD1664  [6]
 LDCM0462  CL7 HEK-293T C718(1.30)  LDD1665  [6]
 LDCM0464  CL71 HEK-293T C718(0.89)  LDD1667  [6]
 LDCM0466  CL73 HEK-293T C718(1.06)  LDD1669  [6]
 LDCM0467  CL74 HEK-293T C718(1.10)  LDD1670  [6]
 LDCM0471  CL78 HEK-293T C718(0.99)  LDD1674  [6]
 LDCM0472  CL79 HEK-293T C718(1.21)  LDD1675  [6]
 LDCM0475  CL81 HEK-293T C718(0.94)  LDD1678  [6]
 LDCM0477  CL83 HEK-293T C718(0.90)  LDD1680  [6]
 LDCM0479  CL85 HEK-293T C718(1.07)  LDD1682  [6]
 LDCM0480  CL86 HEK-293T C718(0.96)  LDD1683  [6]
 LDCM0484  CL9 HEK-293T C718(0.89)  LDD1687  [6]
 LDCM0485  CL90 HEK-293T C718(0.92)  LDD1688  [6]
 LDCM0486  CL91 HEK-293T C718(1.19)  LDD1689  [6]
 LDCM0488  CL93 HEK-293T C718(1.01)  LDD1691  [6]
 LDCM0490  CL95 HEK-293T C718(1.03)  LDD1693  [6]
 LDCM0492  CL97 HEK-293T C718(1.04)  LDD1695  [6]
 LDCM0493  CL98 HEK-293T C718(0.95)  LDD1696  [6]
 LDCM0427  Fragment51 HEK-293T C718(0.97)  LDD1631  [6]
 LDCM0022  KB02 HEK-293T C718(1.08)  LDD1492  [6]
 LDCM0023  KB03 HEK-293T C718(1.05)  LDD1497  [6]
 LDCM0024  KB05 RD C779(2.04)  LDD3401  [1]
 LDCM0109  NEM HeLa N.A.  LDD0229  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Fibroblast growth factor receptor 3 (FGFR3) Tyr protein kinase family P22607
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Glutamate receptor ionotropic, NMDA 2C (GRIN2C) Glutamate-gated ion channel family Q14957
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cytoplasmic dynein 1 heavy chain 1 (DYNC1H1) Dynein heavy chain family Q14204
Phosphatidylinositol 3-kinase regulatory subunit gamma (PIK3R3) PI3K p85 subunit family Q92569
Gelsolin (GSN) Villin/gelsolin family P06396

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
3 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
4 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
5 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
6 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402