Details of the Target

General Information of Target

Target ID LDTP00646
Target Name Alpha-2,8-sialyltransferase 8E (ST8SIA5)
Gene Name ST8SIA5
Gene ID 29906
Synonyms
SIAT8E; Alpha-2,8-sialyltransferase 8E; EC 2.4.99.-; Sialyltransferase 8E; SIAT8-E; Sialyltransferase St8Sia V; ST8SiaV
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MRYADPSANRDLLGSRTLLFIFICAFALVTLLQQILYGRNYIKRYFEFYEGPFEYNSTRC
LELRHEILEVKVLSMVKQSELFDRWKSLQMCKWAMNISEANQFKSTLSRCCNAPAFLFTT
QKNTPLGTKLKYEVDTSGIYHINQEIFRMFPKDMPYYRSQFKKCAVVGNGGILKNSRCGR
EINSADFVFRCNLPPISEKYTMDVGVKTDVVTVNPSIITERFHKLEKWRRPFYRVLQVYE
NASVLLPAFYNTRNTDVSIRVKYVLDDFESPQAVYYFHPQYLVNVSRYWLSLGVRAKRIS
TGLILVTAALELCEEVHLFGFWAFPMNPSGLYITHHYYDNVKPRPGFHAMPSEIFNFLHL
HSRGILRVHTGTCSCC
Target Bioclass
Enzyme
Family
Glycosyltransferase 29 family
Subcellular location
Golgi apparatus membrane
Function Involved in the synthesis of gangliosides GD1c, GT1a, GQ1b, GP1c and GT3 from GD1a, GT1b, GM1b and GD3 respectively.
Uniprot ID
O15466
Ensemble ID
ENST00000315087.12
HGNC ID
HGNC:17827

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CCK81 SNV: p.M1? .
COLO792 SNV: p.E66K; p.L366F .
HCT15 SNV: p.R2C .
HUH28 SNV: p.L290F .
SW1573 SNV: p.S329L .
SW403 SNV: p.V235M .
TOV21G SNV: p.P271Q .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IA-alkyne
 Probe Info  		C164(1.42)  LDD2182  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0625  F8 Ramos C164(0.89)  LDD2187  [1]
 LDCM0572  Fragment10 Ramos C164(0.45)  LDD2189  [1]
 LDCM0573  Fragment11 Ramos C164(0.02)  LDD2190  [1]
 LDCM0574  Fragment12 Ramos C164(0.43)  LDD2191  [1]
 LDCM0575  Fragment13 Ramos C164(1.31)  LDD2192  [1]
 LDCM0576  Fragment14 Ramos C164(1.20)  LDD2193  [1]
 LDCM0579  Fragment20 Ramos C164(0.64)  LDD2194  [1]
 LDCM0580  Fragment21 Ramos C164(0.67)  LDD2195  [1]
 LDCM0582  Fragment23 Ramos C164(0.97)  LDD2196  [1]
 LDCM0578  Fragment27 Ramos C164(0.81)  LDD2197  [1]
 LDCM0586  Fragment28 Ramos C164(0.45)  LDD2198  [1]
 LDCM0588  Fragment30 Ramos C164(0.66)  LDD2199  [1]
 LDCM0589  Fragment31 Ramos C164(1.18)  LDD2200  [1]
 LDCM0590  Fragment32 Ramos C164(0.62)  LDD2201  [1]
 LDCM0468  Fragment33 Ramos C164(1.07)  LDD2202  [1]
 LDCM0596  Fragment38 Ramos C164(0.63)  LDD2203  [1]
 LDCM0566  Fragment4 Ramos C164(0.53)  LDD2184  [1]
 LDCM0610  Fragment52 Ramos C164(1.17)  LDD2204  [1]
 LDCM0614  Fragment56 Ramos C164(1.02)  LDD2205  [1]
 LDCM0569  Fragment7 Ramos C164(1.16)  LDD2186  [1]
 LDCM0571  Fragment9 Ramos C164(0.61)  LDD2188  [1]
 LDCM0022  KB02 Ramos C164(1.42)  LDD2182  [1]
 LDCM0023  KB03 Ramos C164(1.99)  LDD2183  [1]
 LDCM0024  KB05 Ramos C164(1.42)  LDD2185  [1]

References

1 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578