Details of the Target

General Information of Target

Target ID LDTP00568
Target Name Mothers against decapentaplegic homolog 9 (SMAD9)
Gene Name SMAD9
Gene ID 4093
Synonyms
MADH6; MADH9; SMAD8; Mothers against decapentaplegic homolog 9; MAD homolog 9; Mothers against DPP homolog 9; Madh6; SMAD family member 9; SMAD 9; Smad9
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MHSTTPISSLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDSLVKKLKKKKGAMDELERALS
CPGQPSKCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLECCEFPFGSK
QKEVCINPYHYRRVETPVLPPVLVPRHSEYNPQLSLLAKFRSASLHSEPLMPHNATYPDS
FQQPPCSALPPSPSHAFSQSPCTASYPHSPGSPSEPESPYQHSVDTPPLPYHATEASETQ
SGQPVDATADRHVVLSIPNGDFRPVCYEEPQHWCSVAYYELNNRVGETFQASSRSVLIDG
FTDPSNNRNRFCLGLLSNVNRNSTIENTRRHIGKGVHLYYVGGEVYAECVSDSSIFVQSR
NCNYQHGFHPATVCKIPSGCSLKVFNNQLFAQLLAQSVHHGFEVVYELTKMCTIRMSFVK
GWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNPISSVS
Target Type
Literature-reported
Target Bioclass
Transcription factor
Family
Dwarfin/SMAD family
Subcellular location
Cytoplasm
Function Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD).
TTD ID
T75077
Uniprot ID
O15198
DrugMap ID
TTX8EBV
Ensemble ID
ENST00000350148.10
HGNC ID
HGNC:6774

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
FADU SNV: p.Q153Ter .
HCT15 SNV: p.K383N .
HT115 SNV: p.G86V; p.E425D .
IGR37 SNV: p.S103A .
IGR39 SNV: p.S103A .
KPL1 SNV: p.V42L .
MCC26 SNV: p.S164F .
MCF7 SNV: p.V42L .
NCIH358 SNV: p.H130N .
SW1573 SNV: p.R94H .
YSCCC SNV: p.Q240H .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C380(1.78)  LDD3314  [1]
IA-alkyne
 Probe Info  		N.A.  LDD0175  [2]
IPM
 Probe Info  		N.A.  LDD2156  [3]
AOyne
 Probe Info  		15.00  LDD0443  [4]
NAIA_5
 Probe Info  		N.A.  LDD2223  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA HEK-293T C68(2.81)  LDD0371  [6]
 LDCM0026  4SU-RNA+native RNA HEK-293T C68(9.16)  LDD0372  [6]
 LDCM0022  KB02 A2058 C61(2.61)  LDD2253  [1]
 LDCM0023  KB03 A2058 C61(3.67)  LDD2670  [1]
 LDCM0024  KB05 IGR37 C380(1.78)  LDD3314  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein arginine N-methyltransferase 6 (PRMT6) Protein arginine N-methyltransferase family Q96LA8
Lysine-specific histone demethylase 1A (KDM1A) Flavin monoamine oxidase family O60341
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Mothers against decapentaplegic homolog 4 (SMAD4) Dwarfin/SMAD family Q13485
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Progranulin (GRN) Granulin family P28799
Inner nuclear membrane protein Man1 (LEMD3) . Q9Y2U8

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
3 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
4 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
5 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
6 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625