Details of the Target

General Information of Target

Target ID LDTP00530
Target Name Serine/threonine-protein kinase DCLK1 (DCLK1)
Gene Name DCLK1
Gene ID 9201
Synonyms
DCAMKL1; DCDC3A; KIAA0369; Serine/threonine-protein kinase DCLK1; EC 2.7.11.1; Doublecortin domain-containing protein 3A; Doublecortin-like and CAM kinase-like 1; Doublecortin-like kinase 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSFGRDMELEHFDERDKAQRYSRGSRVNGLPSPTHSAHCSFYRTRTLQTLSSEKKAKKVR
FYRNGDRYFKGIVYAISPDRFRSFEALLADLTRTLSDNVNLPQGVRTIYTIDGLKKISSL
DQLVEGESYVCGSIEPFKKLEYTKNVNPNWSVNVKTTSASRAVSSLATAKGSPSEVRENK
DFIRPKLVTIIRSGVKPRKAVRILLNKKTAHSFEQVLTDITDAIKLDSGVVKRLYTLDGK
QVMCLQDFFGDDDIFIACGPEKFRYQDDFLLDESECRVVKSTSYTKIASSSRRSTTKSPG
PSRRSKSPASTSSVNGTPGSQLSTPRSGKSPSPSPTSPGSLRKQRSSQHGGSSTSLASTK
VCSSMDENDGPGEEVSEEGFQIPATITERYKVGRTIGDGNFAVVKECVERSTAREYALKI
IKKSKCRGKEHMIQNEVSILRRVKHPNIVLLIEEMDVPTELYLVMELVKGGDLFDAITST
NKYTERDASGMLYNLASAIKYLHSLNIVHRDIKPENLLVYEHQDGSKSLKLGDFGLATIV
DGPLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCGFPPFRGSGDDQEVLFDQ
ILMGQVDFPSPYWDNVSDSAKELITMMLLVDVDQRFSAVQVLEHPWVNDDGLPENEHQLS
VAGKIKKHFNTGPKPNSTAAGVSVIATTALDKERQVFRRRRNQDVRSRYKAQPAPPELNS
ESEDYSPSSSETVRSPNSPF
Target Type
Literature-reported
Target Bioclass
Enzyme
Family
Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CaMK subfamily
Function Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system.
TTD ID
T42447
Uniprot ID
O15075
DrugMap ID
TTOHTCY
Ensemble ID
ENST00000255448.8
HGNC ID
HGNC:2700
ChEMBL ID
CHEMBL5683

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AGS SNV: p.R510H .
C32 SNV: p.P736S .
COLO679 SNV: p.P585S .
HCT15 SNV: p.L518M .
HT115 SNV: p.V547I .
IM95 SNV: p.N64K .
LS123 SNV: p.A620E .
LS180 SNV: p.V632A .
MEWO SNV: p.S212F .
NALM6 Insertion: p.D599Ter .
OVTOKO SNV: p.R60C .
SG231 SNV: p.A288S .
SISO SNV: p.Q605K .
SW948 SNV: p.C39Ter .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C131(1.23)  LDD3332  [1]
AHL-Pu-1
 Probe Info  		C276(4.80)  LDD0168  [2]
IA-alkyne
 Probe Info  		N.A.  LDD0149  [3]
IPM
 Probe Info  		N.A.  LDD2156  [4]
AOyne
 Probe Info  		15.00  LDD0443  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA HEK-293T C276(4.80)  LDD0168  [2]
 LDCM0026  4SU-RNA+native RNA HEK-293T C276(2.43)  LDD0169  [2]
 LDCM0022  KB02 IPC-298 C131(1.10)  LDD2400  [1]
 LDCM0023  KB03 KATO III C131(0.98)  LDD2833  [1]
 LDCM0024  KB05 MKN45 C131(1.23)  LDD3332  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cell division cycle protein 23 homolog (CDC23) APC8/CDC23 family Q9UJX2
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
EF-hand domain-containing family member C2 (EFHC2) . Q5JST6
Kelch-like protein 15 (KLHL15) . Q96M94

The Drug(s) Related To This Target

Approved
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Fostamatinib Small molecular drug DB12010
Investigative
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Pmid20483621c5g Small molecular drug D0H0LP

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
3 Sequence-Based Prediction of Cysteine Reactivity Using Machine Learning. Biochemistry. 2018 Jan 30;57(4):451-460. doi: 10.1021/acs.biochem.7b00897. Epub 2017 Oct 26.
4 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
5 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.