Details of the Target

General Information of Target

Target ID LDTP00524
Target Name Synemin (SYNM)
Gene Name SYNM
Gene ID 23336
Synonyms
DMN; KIAA0353; SYN; Synemin; Desmuslin
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MLSWRLQTGPEKAELQELNARLYDYVCRVRELERENLLLEEELRGRRGREGLWAEGQARC
AEEARSLRQQLDELSWATALAEGERDALRRELRELQRLDAEERAARGRLDAELGAQQREL
QEALGARAALEALLGRLQAERRGLDAAHERDVRELRARAASLTMHFRARATGPAAPPPRL
REVHDSYALLVAESWRETVQLYEDEVRELEEALRRGQESRLQAEEETRLCAQEAEALRRE
ALGLEQLRARLEDALLRMREEYGIQAEERQRVIDCLEDEKATLTLAMADWLRDYQDLLQV
KTGLSLEVATYRALLEGESNPEIVIWAEHVENMPSEFRNKSYHYTDSLLQRENERNLFSR
QKAPLASFNHSSALYSNLSGHRGSQTGTSIGGDARRGFLGSGYSSSATTQQENSYGKAVS
SQTNVRTFSPTYGLLRNTEAQVKTFPDRPKAGDTREVPVYIGEDSTIARESYRDRRDKVA
AGASESTRSNERTVILGKKTEVKATREQERNRPETIRTKPEEKMFDSKEKASEERNLRWE
ELTKLDKEARQRESQQMKEKAKEKDSPKEKSVREREVPISLEVSQDRRAEVSPKGLQTPV
KDAGGGTGREAEARELRFRLGTSDATGSLQGDSMTETVAENIVTSILKQFTQSPETEASA
DSFPDTKVTYVDRKELPGERKTKTEIVVESKLTEDVDVSDEAGLDYLLSKDIKEVGLKGK
SAEQMIGDIINLGLKGREGRAKVVNVEIVEEPVSYVSGEKPEEFSVPFKVEEVEDVSPGP
WGLVKEEEGYGESDVTFSVNQHRRTKQPQENTTHVEEVTEAGDSEGEQSYFVSTPDEHPG
GHDRDDGSVYGQIHIEEESTIRYSWQDEIVQGTRRRTQKDGAVGEKVVKPLDVPAPSLEG
DLGSTHWKEQARSGEFHAEPTVIEKEIKIPHEFHTSMKGISSKEPRQQLVEVIGQLEETL
PERMREELSALTREGQGGPGSVSVDVKKVQGAGGSSVTLVAEVNVSQTVDADRLDLEELS
KDEASEMEKAVESVVRESLSRQRSPAPGSPDEEGGAEAPAAGIRFRRWATRELYIPSGES
EVAGGASHSSGQRTPQGPVSATVEVSSPTGFAQSQVLEDVSQAARHIKLGPSEVWRTERM
SYEGPTAEVVEVSAGGDLSQAASPTGASRSVRHVTLGPGQSPLSREVIFLGPAPACPEAW
GSPEPGPAESSADMDGSGRHSTFGCRQFHAEKEIIFQGPISAAGKVGDYFATEESVGTQT
SVRQLQLGPKEGFSGQIQFTAPLSDKVELGVIGDSVHMEGLPGSSTSIRHISIGPQRHQT
TQQIVYHGLVPQLGESGDSESTVHGEGSADVHQATHSHTSGRQTVMTEKSTFQSVVSESP
QEDSAEDTSGAEMTSGVSRSFRHIRLGPTETETSEHIAIRGPVSRTFVLAGSADSPELGK
LADSSRTLRHIAPGPKETSFTFQMDVSNVEAIRSRTQEAGALGVSDRGSWRDADSRNDQA
VGVSFKASAGEGDQAHREQGKEQAMFDKKVQLQRMVDQRSVISDEKKVALLYLDNEEEEN
DGHWF
Target Bioclass
Other
Family
Intermediate filament family
Subcellular location
Cytoplasm, cytoskeleton
Function
Type-VI intermediate filament (IF) which plays an important cytoskeletal role within the muscle cell cytoskeleton. It forms heteromeric IFs with desmin and/or vimentin, and via its interaction with cytoskeletal proteins alpha-dystrobrevin, dystrophin, talin-1, utrophin and vinculin, is able to link these heteromeric IFs to adherens-type junctions, such as to the costameres, neuromuscular junctions, and myotendinous junctions within striated muscle cells.
Uniprot ID
O15061
Ensemble ID
ENST00000328642.11
HGNC ID
HGNC:24466

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
BXPC3 SNV: p.L545F .
CAL51 Deletion: p.H842TfsTer47 .
HCT15 SNV: p.E762D .
LU65 SNV: p.A79E DBIA    Probe Info 
MEWO SNV: p.G881D DBIA    Probe Info 
NCIH2172 SNV: p.Q871R DBIA    Probe Info 
OCIAML2 SNV: p.K987R .
PF382 SNV: p.Y472C .
RKO SNV: p.A174T .
SISO SNV: p.D274E DBIA    Probe Info 
TOV21G SNV: p.C275Ter DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		13.45  LDD0402  [1]
AHL-Pu-1
 Probe Info  		C275(10.61)  LDD0170  [2]
DBIA
 Probe Info  		C27(1.06)  LDD0078  [3]
IPM
 Probe Info  		C1225(0.00); C60(0.00)  LDD0147  [4]
TFBX
 Probe Info  		C60(0.00); C230(0.00)  LDD0148  [4]
Acrolein
 Probe Info  		N.A.  LDD0217  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA DM93 C275(10.61)  LDD0170  [2]
 LDCM0026  4SU-RNA+native RNA DM93 C275(6.01); C1225(2.88); C1196(4.03)  LDD0171  [2]
 LDCM0156  Aniline NCI-H1299 11.59  LDD0403  [1]
 LDCM0020  ARS-1620 HCC44 C27(1.06)  LDD0078  [3]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [5]
 LDCM0022  KB02 769-P C230(1.47); C27(1.55)  LDD2246  [6]
 LDCM0023  KB03 769-P C230(1.46); C27(2.00)  LDD2663  [6]
 LDCM0024  KB05 COLO792 C230(2.16)  LDD3310  [6]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Titin (TTN) CAMK Ser/Thr protein kinase family Q8WZ42

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
3 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
4 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
5 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
6 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840