Details of the Target

General Information of Target

Target ID LDTP00356
Target Name Cyclin-dependent kinase 2-associated protein 1 (CDK2AP1)
Gene Name CDK2AP1
Gene ID 8099
Synonyms
CDKAP1; DOC1; Cyclin-dependent kinase 2-associated protein 1; CDK2-associated protein 1; Deleted in oral cancer 1; DOC-1; Putative oral cancer suppressor
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSYKPNLAAHMPAAALNAAGSVHSPSTSMATSSQYRQLLSDYGPPSLGYTQGTGNSQVPQ
SKYAELLAIIEELGKEIRPTYAGSKSAMERLKRGIIHARGLVRECLAETERNARS
Target Bioclass
Other
Family
CDK2AP family
Subcellular location
Nucleus
Function Inhibitor of cyclin-dependent kinase CDK2. Also acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin.
Uniprot ID
O14519
Ensemble ID
ENST00000261692.7
HGNC ID
HGNC:14002
ChEMBL ID
CHEMBL5578

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
Ishikawa (Heraklio) 02 ER SNV: p.S40N .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 11 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
5E-2FA
 Probe Info  		H23(0.00); H10(0.00)  LDD2235  [1]
m-APA
 Probe Info  		N.A.  LDD2231  [1]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [2]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [2]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [2]
JW-RF-010
 Probe Info  		N.A.  LDD0026  [3]
NAIA_5
 Probe Info  		C105(0.00); C116(0.00)  LDD2224  [4]
TFBX
 Probe Info  		N.A.  LDD0027  [3]
IPM
 Probe Info  		N.A.  LDD2156  [5]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [6]
AOyne
 Probe Info  		15.00  LDD0443  [7]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0625  F8 Ramos C105(0.93)  LDD2187  [8]
 LDCM0572  Fragment10 Ramos C105(0.74)  LDD2189  [8]
 LDCM0573  Fragment11 Ramos C105(2.41)  LDD2190  [8]
 LDCM0574  Fragment12 Ramos C105(0.71)  LDD2191  [8]
 LDCM0575  Fragment13 Ramos C105(0.92)  LDD2192  [8]
 LDCM0576  Fragment14 Ramos C105(1.95)  LDD2193  [8]
 LDCM0579  Fragment20 Ramos C105(0.76)  LDD2194  [8]
 LDCM0580  Fragment21 Ramos C105(0.81)  LDD2195  [8]
 LDCM0582  Fragment23 Ramos C105(2.44)  LDD2196  [8]
 LDCM0578  Fragment27 Ramos C105(0.85)  LDD2197  [8]
 LDCM0586  Fragment28 Ramos C105(0.71)  LDD2198  [8]
 LDCM0588  Fragment30 Ramos C105(1.10)  LDD2199  [8]
 LDCM0589  Fragment31 Ramos C105(0.87)  LDD2200  [8]
 LDCM0590  Fragment32 Ramos C105(0.66)  LDD2201  [8]
 LDCM0468  Fragment33 Ramos C105(0.92)  LDD2202  [8]
 LDCM0596  Fragment38 Ramos C105(1.01)  LDD2203  [8]
 LDCM0566  Fragment4 Ramos C105(0.96)  LDD2184  [8]
 LDCM0610  Fragment52 Ramos C105(1.17)  LDD2204  [8]
 LDCM0614  Fragment56 Ramos C105(1.15)  LDD2205  [8]
 LDCM0569  Fragment7 Ramos C105(1.02)  LDD2186  [8]
 LDCM0571  Fragment9 Ramos C105(0.94)  LDD2188  [8]
 LDCM0022  KB02 Ramos C105(1.16)  LDD2182  [8]
 LDCM0023  KB03 Ramos C105(1.11)  LDD2183  [8]
 LDCM0024  KB05 Ramos C105(2.63)  LDD2185  [8]
 LDCM0627  NUDT7-COV-1 HEK-293T C105(0.93)  LDD2206  [9]
 LDCM0628  OTUB2-COV-1 HEK-293T C105(0.37)  LDD2207  [9]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Maspardin (SPG21) AB hydrolase superfamily Q9NZD8
Protein N-terminal glutamine amidohydrolase (NTAQ1) NTAQ1 family Q96HA8
Fibroblast growth factor receptor 3 (FGFR3) Tyr protein kinase family P22607
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
ATP synthase mitochondrial F1 complex assembly factor 2 (ATPAF2) ATP12 family Q8N5M1
MORF4 family-associated protein 1-like 1 (MRFAP1L1) MORF4 family-associated protein family Q96HT8
PRKCA-binding protein (PICK1) . Q9NRD5

References

1 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
2 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
3 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
5 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
6 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
7 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
8 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
9 Rapid Covalent-Probe Discovery by Electrophile-Fragment Screening. J Am Chem Soc. 2019 Jun 5;141(22):8951-8968. doi: 10.1021/jacs.9b02822. Epub 2019 May 22.