Details of the Target

General Information of Target

Target ID LDTP00100
Target Name Tubulin alpha chain-like 3 (TUBAL3)
Gene Name TUBAL3
Gene ID 79861
Synonyms
Tubulin alpha chain-like 3; EC 3.6.5.-
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MRECLSIHIGQAGIQIGDACWELYCLEHGIQPNGVVLDTQQDQLENAKMEHTNASFDTFF
CETRAGKHVPRALFVDLEPTVIDGIRTGQHRSLFHPEQLLSGKEDAANNYARGRYSVGSE
VIDLVLERTRKLAEQCGGLQGFLIFRSFGGGTGSGFTSLLMERLTGEYSRKTKLEFSVYP
APRISTAVVEPYNSVLTTHSTTEHTDCTFMVDNEAVYDICHRKLGVECPSHASINRLVVQ
VVSSITASLRFEGPLNVDLIEFQTNLVPYPRIHFPMTAFAPIVSADKAYHEQFSVSDITT
ACFESSNQLVKCDPRLGKYMACCLLYRGDVVPKEVNAAIAATKSRHSVQFVDWCPTGFKV
GINNRPPTVMPGGDLAKVHRSICMLSNTTAIVEAWARLDHKFDLMYAKRAFLHWYLREGM
EEAEFLEAREDLAALERDYEEVAQSF
Target Bioclass
Enzyme
Family
Tubulin family
Subcellular location
Cytoplasm, cytoskeleton
Function
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Uniprot ID
A6NHL2
Ensemble ID
ENST00000380419.8
HGNC ID
HGNC:23534
ChEMBL ID
CHEMBL3832941

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
ABC1 SNV: p.D329N .
SW756 SNV: p.R128M .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 19 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
Probe 1
 Probe Info  		Y110(20.28); Y406(37.63)  LDD3495  [2]
HHS-475
 Probe Info  		Y406(0.65); Y319(0.71); Y110(0.72); Y326(0.94)  LDD0264  [3]
HHS-465
 Probe Info  		Y110(8.90); Y319(10.00); Y326(10.00); Y406(10.00)  LDD2237  [4]
5E-2FA
 Probe Info  		N.A.  LDD2235  [5]
AMP probe
 Probe Info  		N.A.  LDD0200  [6]
ATP probe
 Probe Info  		K401(0.00); K408(0.00)  LDD0199  [6]
CY-1
 Probe Info  		D399(0.00); H400(0.00); K408(0.00); K401(0.00)  LDD0246  [7]
CY4
 Probe Info  		N.A.  LDD0247  [7]
4-Iodoacetamidophenylacetylene
 Probe Info  		C136(0.00); C383(0.00)  LDD0038  [8]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [8]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [8]
JW-RF-010
 Probe Info  		C322(0.00); C323(0.00)  LDD0026  [9]
NAIA_4
 Probe Info  		C136(0.00); C323(0.00); C322(0.00)  LDD2226  [10]
TFBX
 Probe Info  		C322(0.00); C323(0.00)  LDD0027  [9]
IPM
 Probe Info  		N.A.  LDD2156  [11]
Ox-W18
 Probe Info  		N.A.  LDD2175  [12]
AOyne
 Probe Info  		15.00  LDD0443  [13]
NAIA_5
 Probe Info  		C323(0.00); C322(0.00)  LDD2223  [10]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0573  Fragment11 Ramos C136(0.81)  LDD2190  [14]
 LDCM0576  Fragment14 Ramos C136(1.32)  LDD2193  [14]
 LDCM0569  Fragment7 Ramos C136(2.87)  LDD2186  [14]
 LDCM0116  HHS-0101 DM93 Y406(0.65); Y319(0.71); Y110(0.72); Y326(0.94)  LDD0264  [3]
 LDCM0117  HHS-0201 DM93 Y406(0.53); Y110(0.57); Y319(0.60); Y326(1.06)  LDD0265  [3]
 LDCM0118  HHS-0301 DM93 Y319(0.52); Y406(0.61); Y326(0.72); Y110(0.74)  LDD0266  [3]
 LDCM0119  HHS-0401 DM93 Y319(0.42); Y406(0.49); Y326(0.60); Y110(0.63)  LDD0267  [3]
 LDCM0120  HHS-0701 DM93 Y110(0.41); Y319(0.53); Y406(0.55); Y326(0.76)  LDD0268  [3]
 LDCM0022  KB02 Ramos C136(0.44)  LDD2182  [14]
 LDCM0023  KB03 Ramos C136(0.25)  LDD2183  [14]
 LDCM0024  KB05 Ramos C136(0.36)  LDD2185  [14]

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
3 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
4 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
5 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
6 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
7 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
8 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
9 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
10 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
11 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
12 Oxidative cyclization reagents reveal tryptophan cation- interactions. Nature. 2024 Mar;627(8004):680-687. doi: 10.1038/s41586-024-07140-6. Epub 2024 Mar 6.
Mass spectrometry data entry: PXD001377 , PXD005252
13 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
14 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578