Details of the Target

General Information of Target

Target ID LDTP00083
Target Name Coiled-coil domain-containing protein 88B (CCDC88B)
Gene Name CCDC88B
Gene ID 283234
Synonyms
BRLZ; Coiled-coil domain-containing protein 88B; Brain leucine zipper domain-containing protein; Gipie; Hook-related protein 3; HkRP3
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEGGKGPRLRDFLSGSLATWALGLAGLVGEAEDSEGEEEEEEEEPPLWLEKRFLRLSDGA
LLLRVLGIIAPSSRGGPRMLRGLDGPAAWRVWNLNHLWGRLRDFYQEELQLLILSPPPDL
QTLGFDPLSEEAVEQLEGVLRLLLGASVQCEHRELFIRHIQGLSLEVQSELAAAIQEVTQ
PGAGVVLALSGPDPGELAPAELEMLSRSLMGTLSKLARERDLGAQRLAELLLEREPLCLR
PEAPSRAPAEGPSHHLALQLANAKAQLRRLRQELEEKAELLLDSQAEVQGLEAEIRRLRQ
EAQALSGQAKRAELYREEAEALRERAGRLPRLQEELRRCRERLQAAEAYKSQLEEERVLS
GVLEASKALLEEQLEAARERCARLHETQRENLLLRTRLGEAHAELDSLRHQVDQLAEENV
ELELELQRSLEPPPGSPGEAPLAGAAPSLQDEVREAEAGRLRTLERENRELRGLLQVLQG
QPGGQHPLLEAPREDPVLPVLEEAPQTPVAFDHSPQGLVQKARDGGPQALDLAPPALDSV
LEASAECPQAPDSDPQEAESPLQAAAMDPQASDWSPQESGSPVETQESPEKAGRRSSLQS
PASVAPPQGPGTKIQAPQLLGGETEGREAPQGELVPEAWGLRQEGPEHKPGPSEPSSVQL
EEQEGPNQGLDLATGQAEAREHDQRLEGTVRDPAWQKPQQKSEGALEVQVWEGPIPGESL
ASGVAEQEALREEVAQLRRKAEALGDELEAQARKLEAQNTEAARLSKELAQARRAEAEAH
REAEAQAWEQARLREAVEAAGQELESASQEREALVEALAAAGRERRQWEREGSRLRAQSE
AAEERMQVLESEGRQHLEEAERERREKEALQAELEKAVVRGKELGDRLEHLQRELEQAAL
ERQEFLREKESQHQRYQGLEQRLEAELQAAATSKEEALMELKTRALQLEEELFQLRQGPA
GLGPKKRAEPQLVETQNVRLIEVERSNAMLVAEKAALQGQLQHLEGQLGSLQGRAQELLL
QSQRAQEHSSRLQAEKSVLEIQGQELHRKLEVLEEEVRAARQSQEETRGQQQALLRDHKA
LAQLQRRQEAELEGLLVRHRDLKANMRALELAHRELQGRHEQLQAQRASVEAQEVALLAE
RERLMQDGHRQRGLEEELRRLQSEHDRAQMLLAELSRERGELQGERGELRGRLARLELER
AQLEMQSQQLRESNQQLDLSACRLTTQCELLTQLRSAQEEENRQLLAEVQALSRENRELL
ERSLESRDHLHREQREYLDQLNALRREKQKLVEKIMDQYRVLEPVPLPRTKKGSWLADKV
KRLMRPRREGGPPGGLRLGADGAGSTESLGGPPETELPEGREADGTGSPSPAPMRRAQSS
LCLRDETLAGGQRRKLSSRFPVGRSSESFSPGDTPRQRFRQRHPGPLGAPVSHSKGPGVG
WENSAETLQEHETDANREGPEVQEPEKRPLTPSLSQ
Target Bioclass
Other
Family
CCDC88 family
Subcellular location
Membrane
Function
Acts as a positive regulator of T-cell maturation and inflammatory function. Required for several functions of T-cells, in both the CD4(+) and the CD8(+) compartments and this includes expression of cell surface markers of activation, proliferation, and cytokine production in response to specific or non-specific stimulation. Enhances NK cell cytotoxicity by positively regulating polarization of microtubule-organizing center (MTOC) to cytotoxic synapse, lytic granule transport along microtubules, and dynein-mediated clustering to MTOC. Interacts with HSPA5 and stabilizes the interaction between HSPA5 and ERN1, leading to suppression of ERN1-induced JNK activation and endoplasmic reticulum stress-induced apoptosis.
Uniprot ID
A6NC98
Ensemble ID
ENST00000301897.5
HGNC ID
HGNC:26757

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 SNV: p.E279K .
8305C SNV: p.E1273K .
CAL51 SNV: p.E1212K .
CAL62 SNV: p.G1334E .
COLO678 SNV: p.K277M; p.E279A .
HCC827 SNV: p.P244S .
HCT116 Deletion: p.G4AfsTer10 .
HCT15 SNV: p.P646H; p.E993D; p.L1252M .
ICC12 SNV: p.P46T .
JURKAT SNV: p.L408M .
KELLY SNV: p.A929E .
LOXIMVI SNV: p.E850K .
MEC1 SNV: p.A312T DBIA    Probe Info 
MELHO SNV: p.A228G .
MEWO Substitution: p.G59K .
MFE319 SNV: p.I69V; p.E747K .
MOLT4 SNV: p.R52K .
NCIH146 SNV: p.E824K
Substitution: p.D886S		 .
NCIH196 Substitution: p.D886S .
NCIH1993 SNV: p.L384R .
NCIH2172 SNV: p.T122P .
NCIH2286 SNV: p.G704V .
OVK18 SNV: p.R1243Q .
RAMOS Substitution: p.D886S .
SF295 SNV: p.E896Ter .
SKOV3 Substitution: p.D886S .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C1222(17.33); C1382(46.37)  LDD0209  [1]
IA-alkyne
 Probe Info  		C1382(12.22)  LDD1704  [2]
Methacrolein
 Probe Info  		N.A.  LDD0218  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0572  Fragment10 Ramos C1382(1.47)  LDD2189  [4]
 LDCM0574  Fragment12 Ramos C1222(0.89); C1382(0.88)  LDD2191  [4]
 LDCM0575  Fragment13 Ramos C1222(0.99); C1382(0.68)  LDD2192  [4]
 LDCM0576  Fragment14 Ramos C1382(0.84)  LDD2193  [4]
 LDCM0579  Fragment20 Ramos C1222(0.87); C1382(0.91)  LDD2194  [4]
 LDCM0580  Fragment21 Ramos C1382(0.81)  LDD2195  [4]
 LDCM0582  Fragment23 Ramos C1382(1.01)  LDD2196  [4]
 LDCM0578  Fragment27 Ramos C1222(1.18); C1382(0.86)  LDD2197  [4]
 LDCM0586  Fragment28 Ramos C1222(0.67); C1382(1.10)  LDD2198  [4]
 LDCM0588  Fragment30 Ramos C1382(0.98)  LDD2199  [4]
 LDCM0589  Fragment31 Ramos C1382(0.75)  LDD2200  [4]
 LDCM0590  Fragment32 Ramos C1222(1.40); C1382(2.28)  LDD2201  [4]
 LDCM0468  Fragment33 Ramos C1382(1.40)  LDD2202  [4]
 LDCM0596  Fragment38 Ramos C1382(0.78)  LDD2203  [4]
 LDCM0566  Fragment4 Ramos C1382(1.06)  LDD2184  [4]
 LDCM0610  Fragment52 Ramos C1222(1.43)  LDD2204  [4]
 LDCM0614  Fragment56 Ramos C1222(0.79); C1382(1.14)  LDD2205  [4]
 LDCM0569  Fragment7 Ramos C1382(1.04)  LDD2186  [4]
 LDCM0571  Fragment9 Ramos C1382(1.07)  LDD2188  [4]
 LDCM0022  KB02 T cell-activated C1382(12.22)  LDD1704  [2]
 LDCM0023  KB03 Jurkat C1222(17.33); C1382(46.37)  LDD0209  [1]
 LDCM0024  KB05 OCI-Ly3 C358(1.85)  LDD3372  [5]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 9 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Nucleoside diphosphate kinase homolog 7 (NME7) NDK family Q9Y5B8
Ubiquitin carboxyl-terminal hydrolase 2 (USP2) Peptidase C19 family O75604
Cyclin-dependent kinase 18 (CDK18) CMGC Ser/Thr protein kinase family Q07002
Exosome complex component RRP46 (EXOSC5) RNase PH family Q9NQT4
GTP-binding protein GEM (GEM) RGK family P55040
Tumor susceptibility gene 101 protein (TSG101) Ubiquitin-conjugating enzyme family Q99816
E3 ubiquitin-protein ligase LNX (LNX1) . Q8TBB1
Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (PIN1) . Q13526
RalA-binding protein 1 (RALBP1) . Q15311
Transcription factor
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Homeobox protein Hox-B5 (HOXB5) Antp homeobox family P09067
Teashirt homolog 2 (TSHZ2) Teashirt C2H2-type zinc-finger protein family Q9NRE2
Other
Click To Hide/Show 21 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein BEX2 (BEX2) BEX family Q9BXY8
Testis-specific gene 10 protein (TSGA10) CEP135/TSGA10 family Q9BZW7
CWF19-like protein 2 (CWF19L2) CWF19 family Q2TBE0
Protein FAM161A (FAM161A) FAM161 family Q3B820
Protein FAM161B (FAM161B) FAM161 family Q96MY7
Lamin-B1 (LMNB1) Intermediate filament family P20700
Lamin-B2 (LMNB2) Intermediate filament family Q03252
Janus kinase and microtubule-interacting protein 2 (JAKMIP2) JAKMIP family Q96AA8
RAD50-interacting protein 1 (RINT1) RINT1 family Q6NUQ1
Beta-taxilin (TXLNB) Taxilin family Q8N3L3
AFG2-interacting ribosome maturation factor (AIRIM) . Q9NX04
Differentially expressed in FDCP 6 homolog (DEF6) . Q9H4E7
KN motif and ankyrin repeat domain-containing protein 2 (KANK2) . Q63ZY3
Phostensin (PPP1R18) . Q6NYC8
Rhombotin-1 (LMO1) . P25800
RNA-binding protein 7 (RBM7) . Q9Y580
SH2 domain-containing protein 4A (SH2D4A) . Q9H788
SH3 domain-containing kinase-binding protein 1 (SH3KBP1) . Q96B97
TBC1 domain family member 22B (TBC1D22B) . Q9NU19
Testis-specific protein 10-interacting protein (TSGA10IP) . Q3SY00
TRAF3-interacting JNK-activating modulator (TRAF3IP3) . Q9Y228

References

1 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
2 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
3 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
4 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
5 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840