Details of the Target

General Information of Target

Target ID LDTP17138
Target Name Zinc-regulated GTPase metalloprotein activator 1F (ZNG1F)
Gene Name ZNG1F
Gene ID 644019
Synonyms
CBWD6; CBWD7; Zinc-regulated GTPase metalloprotein activator 1F; EC 3.6.5.-; Cobalamin synthase W domain-containing protein 6; COBW domain-containing protein 6
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGKCSGRCTLVAFCCLQLVAALERQIFDFLGYQWAPILANFLHIMAVILGIFGTVQYRSR
YLILYAAWLVLWVGWNAFIICFYLEVGQLSQDRDFIMTFNTSLHRSWWMENGPGCLVTPV
LNSRLALEDHHVISVTGCLLDYPYIEALSSALQIFLALFGFVFACYVSKVFLEEEDSFDF
IGGFDSYGYQAPQKTSHLQLQPLYTSG
Target Bioclass
Enzyme
Family
SIMIBI class G3E GTPase family, ZNG1 subfamily
Subcellular location
Nucleus
Function
Zinc chaperone that directly transfers zinc cofactor to target metalloproteins, thereby activating them. Catalyzes zinc insertion into the active site of methionine aminopeptidase METAP1, which function to cleave the initiator methionine from polypeptides during or after protein translation. Mechanistically, the N-terminal psi-PxLVp motif binds to the C6H2-type zinc finger of inactive form of METAP1. After formation of the docked complex, zinc is transferred from the CXCC motif in the GTPase domain of ZNG1F to the zinc binding site in the peptidase domain of METAP1 in a process requiring GTP hydrolysis. GTP/GDP exchange is required for release of active METAP1.
Uniprot ID
Q4V339
Ensemble ID
ENST00000377391.8
HGNC ID
HGNC:31978

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [1]
IA-alkyne
 Probe Info  		N.A.  LDD2241  [1]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [1]
NAIA_5
 Probe Info  		N.A.  LDD2223  [2]

References

1 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
2 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264