Details of the Target

General Information of Target

Target ID LDTP16234
Target Name DENN domain-containing protein 2A (DENND2A)
Gene Name DENND2A
Gene ID 27147
Synonyms
KIAA1277; DENN domain-containing protein 2A
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MKLGKVEFCHFLQLIALFLCFSGMSQAELSRSRSKPYFQSGRSRTKRSWVWNQFFVLEEY
MGSDPLYVGKLHSDVDKGDGSIKYILSGEGASSIFIIDENTGDIHATKRLDREEQAYYTL
RAQALDRLTNKPVEPESEFVIKIQDINDNEPKFLDGPYTAGVPEMSPVGTSVVQVTATDA
DDPTYGNSARVVYSILQGQPYFSVEPKTGVIKTALPNMDREAKDQYLLVIQAKDMVGQNG
GLSGTTSVTVTLTDVNDNPPRFPRRSYQYNVPESLPVASVVARIKAADADIGANAEMEYK
IVDGDGLGIFKISVDKETQEGIITIQKELDFEAKTSYTLRIEAANKDADPRFLSLGPFSD
TTTVKIIVEDVDEPPVFSSPLYPMEVSEATQVGNIIGTVAAHDPDSSNSPVRYSIDRNTD
LERYFNIDANSGVITTAKSLDRETNAIHNITVLAMESQNPSQVGRGYVAITILDINDNAP
EFAMDYETTVCENAQPGQVIQKISAVDKDEPSNGHQFYFSLTTDATNNHNFSLKDNKDNT
ASILTRRNGFRRQEQSVYYLPIFIVDSGSPSLSSTNTLTIRVCDCDADGVAQTCNAEAYV
LPAGLSTGALIAILACVLTLLVLILLIVTMRRRKKEPLIFDEERDIRENIVRYDDEGGGE
EDTEAFDMAALRNLNVIRDTKTRRDVTPEIQFLSRPAFKSIPDNVIFREFIWERLKEADV
DPGAPPYDSLQTYAFEGNGSVAESLSSLDSISSNSDQNYDYLSDWGPRFKRLADMYGTGQ
ESLYS
Target Bioclass
Other
Subcellular location
Cytoplasm, cytoskeleton
Function
Guanine nucleotide exchange factor (GEF) which may activate RAB9A and RAB9B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. May play a role in late endosomes back to trans-Golgi network/TGN transport.
Uniprot ID
Q9ULE3
Ensemble ID
ENST00000275884.10
HGNC ID
HGNC:22212

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A2780 SNV: p.P721S .
CAL78 SNV: p.K717N .
CHL1 SNV: p.E527K DBIA    Probe Info 
COLO679 SNV: p.S408F .
CORL23 SNV: p.E292D .
FADU SNV: p.E371Ter; p.L399F .
ICC4 SNV: p.R732G .
IGROV1 SNV: p.D86G .
LNCaP clone FGC SNV: p.V904A .
MOLM16 SNV: p.G198V .
NCIH1299 SNV: p.L964Q .
NCIH1703 SNV: p.R216S; p.R732P .
NCIH1793 SNV: p.F674I .
NCIH2172 SNV: p.L418S .
OVISE Deletion: p.A580LfsTer13 .
REH SNV: p.F955L .
RVH421 SNV: p.S939F .
SH4 SNV: p.S344F .
TE10 SNV: p.D155N .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K997(0.32)  LDD0277  [1]
IA-alkyne
 Probe Info  		C190(0.54)  LDD2157  [2]
DBIA
 Probe Info  		C673(2.75)  LDD3365  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 786-O C673(2.24)  LDD2247  [3]
 LDCM0023  KB03 A101D C673(4.22)  LDD2667  [3]
 LDCM0024  KB05 OVCAR-3 C673(2.75)  LDD3365  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
SH3 domain-containing kinase-binding protein 1 (SH3KBP1) . Q96B97

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 From chemoproteomic-detected amino acids to genomic coordinates: insights into precise multi-omic data integration. Mol Syst Biol. 2021 Feb;17(2):e9840. doi: 10.15252/msb.20209840.
Mass spectrometry data entry: PXD022151
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840