Details of the Target

General Information of Target

Target ID LDTP15179
Target Name SPOC domain-containing protein 1 (SPOCD1)
Gene Name SPOCD1
Gene ID 90853
Synonyms
SPOC domain-containing protein 1
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MVKQTIQIFARVKPPVRKHQQGIYSIDEDEKLIPSLEIILPRDLADGFVNNKRESYKFKF
QRIFDQDANQETVFENIAKPVAGSVLAGYNGTIFAYGQTGSGKTFTITGGAERYSDRGII
PRTLSYIFEQLQKDSSKIYTTHISYLEIYNECGYDLLDPRHEASSLEDLPKVTILEDPDQ
NIHLKNLTLHQATTEEEALNLLFLGDTNRMIAETPMNQASTRSHCIFTIHLSSKEPGSAT
VRHAKLHLVDLAGSERVAKTGVGGHLLTEAKYINLSLHYLEQVIIALSEKHRSHIPYRNS
MMTSVLRDSLGGNCMTTMIATLSLEKRNLDESISTCRFAQRVALIKNEAVLNEEINPRLV
IKRLQKEIQELKDELAMVTGEQRTEALTEAELLQLEKLITSFLEDQDSDSRLEVGADMRK
VHHCFHHLKKLLNDKKILENNTVSSESKDQDCQEPLKEEEYRKLRDILKQRDNEINILVN
MLKKEKKKAQEALHLAGMDRREFRQSQSPPFRLGNPEEGQRMRLSSAPSQAQDFSILGKR
SSLLHKKIGMREEMSLGCQEAFEIFKRDHADSVTIDDNKQILKQRFSEAKALGESINEAR
SKIGHLKEEITQRHIQQVALGISENMAVPLMPDQQEEKLRSQLEEEKRRYKTMFTRLKAL
KVEIEHLQLLMDKAKVKLQKEFEVWWAEEATNLQVNSPAVNSLDHTKPFLQTSDSQHEWS
QLLSNKSSGGWEVQDQGTGRFDVCDVNARKILPSPCPSPHSQKQSSTSTPLEDSIPKRPV
SSIPLTGDSQTDSDIIAFIKARQSILQKQCLGSN
Target Bioclass
Other
Subcellular location
Nucleus
Function
Essential excecutor of PIWIL4-piRNA pathway directed transposon DNA methylation and silencing in the male embryonic germ cells. Associates with the de novo DNA methylation machinery and repressive chromatin remodeling complexes. Tethering of PIWIL4 to a nascent transposable element transcript recruits repressive chromatin remodeling activities and the de novo methylation apparatus through SPOCD1. Not required for piRNA biosynthesis.
Uniprot ID
Q6ZMY3
Ensemble ID
ENST00000257100.7
HGNC ID
HGNC:26338

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCC1954 SNV: p.P105S; p.R671W .
HCT15 SNV: p.S389R .
HT3 SNV: p.R206T .
JURKAT SNV: p.A154V; p.S310N .
KASUMI2 SNV: p.K734N .
L428 Deletion: p.G1116AfsTer63 .
MCC13 SNV: p.D814N .
MELJUSO SNV: p.E1099K .
MFE319 SNV: p.A343T .
MOLT4 Deletion: p.L401WfsTer25
SNV: p.G491D;p.A531S;p.S1077I		 .
NCIH1155 SNV: p.P702L .
PANC0403 SNV: p.Q355H .
RL952 SNV: p.A343V .
SKMEL2 SNV: p.E841K; p.P1178H .
SUPT1 Insertion: p.L83FfsTer43
SNV: p.K794N		 .
SW837 SNV: p.A358S .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C933(1.91)  LDD3311  [1]
Acrolein
 Probe Info  		N.A.  LDD0217  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 G361 C933(1.56)  LDD2477  [1]
 LDCM0023  KB03 G361 C933(1.62)  LDD2894  [1]
 LDCM0024  KB05 G361 C933(1.91)  LDD3311  [1]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.