General Information of Target

Target ID LDTP15134
Target Name Solute carrier family 25 member 47 (SLC25A47)
Gene Name SLC25A47
Gene ID 283600
Synonyms
C14orf68; HDMCP; Solute carrier family 25 member 47; Hepatocellular carcinoma down-regulated mitochondrial carrier protein; Mitochondrial NAD(+) transporter SLC25A47
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MQMARLLGLCAWARKSVRMASSRMTRRDPLTNKVALVTASTDGIGFAIARRLAQDRAHVV
VSSRKQQNVDQAVATLQGEGLSVTGTVCHVGKAEDRERLVAMAVKLHGGIDILVSNAAVN
PFFGSLMDVTEEVWDKTLDINVKAPALMTKAVVPEMEKRGGGSVVIVSSIAAFSPSPGFS
PYNVSKTALLGLNNTLAIELAPRNIRVNCLHLDLSRLASAGCSGWTRKKRKA
Target Bioclass
Transporter and channel
Family
Mitochondrial carrier (TC 2.A.29) family
Subcellular location
Mitochondrion inner membrane
Function
Mitochondrial NAD(+) transporter that acts as a 'metabolic gate' in hepatic lipogenesis. Provides NAD(+) substrate to mitochondrial SIRT3 deacetylase and enables its NAD(+)-dependent activities in mitochondrial energy metabolism. This triggers downstream activation of PRKAA1/AMPK-alpha signaling cascade that negatively regulates sterol regulatory element-binding protein (SREBP) transcriptional activities and ATP-consuming lipogenesis to restore cellular energy balance. May transport other mitochondrial metabolites having an aromatic nucleotide and phosphate groups, such as acetyl-CoA. Does not transport amino acids. The transport mechanism remains to be elucidated.
Uniprot ID
Q6Q0C1
Ensemble ID
ENST00000361529.5
HGNC ID
HGNC:20115

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
JURKAT SNV: p.V240M .
NCIH1993 SNV: p.W37R .
RL952 SNV: p.M147I .
SCC25 SNV: p.R214P .
SKMEL3 SNV: p.S64F .
TOV21G SNV: p.A129S; p.L245M .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
BTD
 Probe Info 
C165(0.65)  LDD2100  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0507  Nucleophilic fragment 16b MDA-MB-231 C165(0.65)  LDD2100  [1]

References

1 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761