Details of the Target

General Information of Target

Target ID LDTP15096
Target Name S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1B (TYW1B)
Gene Name TYW1B
Gene ID 441250
Synonyms
RSAFD2; S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1B; EC 4.1.3.44; Radical S-adenosyl methionine and flavodoxin domain-containing protein 2; tRNA wybutosine-synthesizing protein 1 homolog B
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MVSWMISRAVVLVFGMLYPAYYSYKAVKTKNVKEYVRWMMYWIVFALYTVIETVADQTVA
WFPLYYELKIAFVIWLLSPYTKGASLIYRKFLHPLLSSKEREIDDYIVQAKERGYETMVN
FGRQGLNLAATAAVTAAVKSQGAITERLRSFSMHDLTTIQGDEPVGQRPYQPLPEAKKKS
KPAPSESAGYGIPLKDGDEKTDEEAEGPYSDNEMLTHKGLRRSQSMKSVKTTKGRKEVRY
GSLKYKVKKRPQVYF
Target Bioclass
Enzyme
Family
TYW1 family
Function
Probable component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the condensation of N-methylguanine with 2 carbon atoms from pyruvate to form the tricyclic 4-demethylwyosine, an intermediate in wybutosine biosynthesis.
Uniprot ID
Q6NUM6
Ensemble ID
ENST00000620995.5
HGNC ID
HGNC:33908

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HT115 SNV: p.E78Ter .
JURKAT SNV: p.W104Ter .
LUDLU1 SNV: p.G599C .
MCC13 SNV: p.H259Y DBIA    Probe Info 
NCIH146 SNV: p.N143Y DBIA    Probe Info 
NCIH2286 Substitution: p.R164L .
NUGC3 SNV: p.S443F .
SNU423 SNV: p.D519Y .
SW756 SNV: p.N449Y .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C215(1.34)  LDD3314  [1]
IPM
 Probe Info  		C440(5.00)  LDD1702  [2]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [3]
NAIA_5
 Probe Info  		C215(0.00); C416(0.00)  LDD2223  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C440(5.00)  LDD1702  [2]
 LDCM0022  KB02 22RV1 C215(1.76)  LDD2243  [1]
 LDCM0023  KB03 22RV1 C215(2.03)  LDD2660  [1]
 LDCM0024  KB05 IGR37 C215(1.34)  LDD3314  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

GPCR
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Taste receptor type 2 member 19 (TAS2R19) G-protein coupled receptor T2R family P59542

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264