Details of the Target

General Information of Target

Target ID LDTP14425
Target Name Nck-associated protein 5 (NCKAP5)
Gene Name NCKAP5
Gene ID 344148
Synonyms
ERIH; NAP5; Nck-associated protein 5; NAP-5; Peripheral clock protein
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MDGKPATRKGPDFCSLRYGLALIMHFSNFTMITQRVSLSIAIIAMVNTTQQQGLSNASTE
GPVADAFNNSSISIKEFDTKASVYQWSPETQGIIFSSINYGIILTLIPSGYLAGIFGAKK
MLGAGLLISSLLTLFTPLAADFGVILVIMVRTVQGMAQGMAWTGQFTIWAKWAPPLERSK
LTTIAGSGSAFGSFIILCVGGLISQALSWPFIFYIFGSTGCVCCLLWFTVIYDDPMHHPC
ISVREKEHILSSLAQQPSSPGRAVPIKAMVTCLPLWAIFLGFFSHFWLCTIILTYLPTYI
STLLHVNIRDSGVLSSLPFIAAASCTILGGQLADFLLSRNLLRLITVRKLFSSLGLLLPS
ICAVALPFVASSYVITIILLILIPGTSNLCDSGFIINTLDIAPRYASFLMGISRGFGLIA
GIISSTATGFLISQVGPVY
Target Bioclass
Other
Uniprot ID
O14513
Ensemble ID
ENST00000409213.5
HGNC ID
HGNC:29847

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 SNV: p.V960I .
AN3CA SNV: p.V321A .
CAL120 SNV: p.T1072N .
CCK81 SNV: p.G1035W; p.R1781H .
COLO792 SNV: p.S781L DBIA    Probe Info 
ETK1 SNV: p.E187K .
HCT116 SNV: p.Q685Ter .
HCT15 SNV: p.H70Q; p.S125I; p.G1721R .
HEC1 SNV: p.R1781H .
HEC1B SNV: p.R1781H .
HGC27 SNV: p.N1623S .
HT SNV: p.S351P .
HT115 SNV: p.F731L .
HT29 SNV: p.S174C .
IPC298 SNV: p.P1070S .
KMS27 SNV: p.I32V .
LOXIMVI SNV: p.P1679A .
LS180 SNV: p.S895P .
MONOMAC6 SNV: p.P1829L .
NB4 SNV: p.A1228E .
NCIH358 SNV: p.Q248Ter .
NCIH716 SNV: p.P262L .
SSP25 SNV: p.E187K .
SW756 SNV: p.S580Ter .
TOV21G Insertion: p.N1669KfsTer21
SNV: p.R10M		 .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C1642(0.61)  LDD3344  [1]
N1
 Probe Info  		Q766(0.00); Q769(0.00)  LDD0245  [2]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0367  CL1 HEK-293T C719(0.95)  LDD1571  [4]
 LDCM0370  CL101 HEK-293T C719(0.97)  LDD1574  [4]
 LDCM0374  CL105 HEK-293T C719(1.04)  LDD1578  [4]
 LDCM0378  CL109 HEK-293T C719(1.01)  LDD1582  [4]
 LDCM0383  CL113 HEK-293T C719(0.96)  LDD1587  [4]
 LDCM0387  CL117 HEK-293T C719(0.98)  LDD1591  [4]
 LDCM0392  CL121 HEK-293T C719(0.91)  LDD1596  [4]
 LDCM0396  CL125 HEK-293T C719(1.01)  LDD1600  [4]
 LDCM0400  CL13 HEK-293T C719(0.91)  LDD1604  [4]
 LDCM0413  CL25 HEK-293T C719(0.95)  LDD1617  [4]
 LDCM0426  CL37 HEK-293T C719(1.00)  LDD1630  [4]
 LDCM0439  CL49 HEK-293T C719(1.02)  LDD1643  [4]
 LDCM0453  CL61 HEK-293T C719(1.11)  LDD1656  [4]
 LDCM0466  CL73 HEK-293T C719(1.01)  LDD1669  [4]
 LDCM0479  CL85 HEK-293T C719(1.14)  LDD1682  [4]
 LDCM0492  CL97 HEK-293T C719(0.91)  LDD1695  [4]
 LDCM0022  KB02 A2058 C1642(2.06)  LDD2253  [1]
 LDCM0023  KB03 A2058 C1642(1.64)  LDD2670  [1]
 LDCM0024  KB05 NCI-H146 C1642(0.61)  LDD3344  [1]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
4 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402