Details of the Target

General Information of Target

Target ID LDTP14060
Target Name G-protein-signaling modulator 3 (GPSM3)
Gene Name GPSM3
Gene ID 63940
Synonyms
AGS4; C6orf9; G18; G-protein-signaling modulator 3; Activator of G-protein signaling 4; G18.1b; Protein G18
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MASPPRHGPPGPASGDGPNLNNNNNNNNHSVRKCGYLRKQKHGHKRFFVLRGPGAGGDEA
TAGGGSAPQPPRLEYYESEKKWRSKAGAPKRVIALDCCLNINKRADAKHKYLIALYTKDE
YFAVAAENEQEQEGWYRALTDLVSEGRAAAGDAPPAAAPAASCSASLPGALGGSAGAAGA
EDSYGLVAPATAAYREVWQVNLKPKGLGQSKNLTGVYRLCLSARTIGFVKLNCEQPSVTL
QLMNIRRCGHSDSFFFIEVGRSAVTGPGELWMQADDSVVAQNIHETILEAMKALKELFEF
RPRSKSQSSGSSATHPISVPGARRHHHLVNLPPSQTGLVRRSRTDSLAATPPAAKCSSCR
VRTASEGDGGAAAGAAAAGARPVSVAGSPLSPGPVRAPLSRSHTLSGGCGGRGSKVALLP
AGGALQHSRSMSMPVAHSPPAATSPGSLSSSSGHGSGSYPPPPGPHPPLPHPLHHGPGQR
PSSGSASASGSPSDPGFMSLDEYGSSPGDLRAFCSHRSNTPESIAETPPARDGGGGGEFY
GYMTMDRPLSHCGRSYRRVSGDAAQDLDRGLRKRTYSLTTPARQRPVPQPSSASLDEYTL
MRATFSGSAGRLCPSCPASSPKVAYHPYPEDYGDIEIGSHRSSSSNLGADDGYMPMTPGA
ALAGSGSGSCRSDDYMPMSPASVSAPKQILQPRAAAAAAAAVPSAGPAGPAPTSAAGRTF
PASGGGYKASSPAESSPEDSGYMRMWCGSKLSMEHADGKLLPNGDYLNVSPSDAVTTGTP
PDFFSAALHPGGEPLRGVPGCCYSSLPRSYKAPYTCGGDSDQYVLMSSPVGRILEEERLE
PQATPGPSQAASAFGAGPTQPPHPVVPSPVRPSGGRPEGFLGQRGRAVRPTRLSLEGLPS
LPSMHEYPLPPEPKSPGEYINIDFGEPGARLSPPAPPLLASAASSSSLLSASSPASSLGS
GTPGTSSDSRQRSPLSDYMNLDFSSPKSPKPGAPSGHPVGSLDGLLSPEASSPYPPLPPR
PSASPSSSLQPPPPPPAPGELYRLPPASAVATAQGPGAASSLSSDTGDNGDYTEMAFGVA
ATPPQPIAAPPKPEAARVASPTSGVKRLSLMEQVSGVEAFLQASQPPDPHRGAKVIRADP
QGGRRRHSSETFSSTTTVTPVSPSFAHNPKRHNSASVENVSLRKSSEGGVGVGPGGGDEP
PTSPRQLQPAPPLAPQGRPWTPGQPGGLVGCPGSGGSPMRRETSAGFQNGLNYIAIDVRE
EPGLPPQPQPPPPPLPQPGDKSSWGRTRSLGGLISAVGVGSTGGGCGGPGPGALPPANTY
ASIDFLSHHLKEATIVKE
Target Bioclass
Other
Subcellular location
Cytoplasm
Function Interacts with subunit of G(i) alpha proteins and regulates the activation of G(i) alpha proteins.
Uniprot ID
Q9Y4H4
Ensemble ID
ENST00000375040.8
HGNC ID
HGNC:13945

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C160(0.94)  LDD3403  [1]
IA-alkyne
 Probe Info  		C116(5.46)  LDD1704  [2]
IPM
 Probe Info  		C116(1.44)  LDD1702  [3]
NAIA_4
 Probe Info  		N.A.  LDD2226  [4]
Compound 10
 Probe Info  		N.A.  LDD2216  [5]
Compound 11
 Probe Info  		N.A.  LDD2213  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C116(1.44)  LDD1702  [3]
 LDCM0625  F8 Ramos C116(2.09)  LDD2187  [6]
 LDCM0572  Fragment10 Ramos C116(0.78)  LDD2189  [6]
 LDCM0573  Fragment11 Ramos C160(16.89); C116(0.64)  LDD2190  [6]
 LDCM0574  Fragment12 Ramos C116(0.59)  LDD2191  [6]
 LDCM0576  Fragment14 Ramos C160(0.74); C116(0.82)  LDD2193  [6]
 LDCM0579  Fragment20 Ramos C116(0.76)  LDD2194  [6]
 LDCM0580  Fragment21 Ramos C116(0.50)  LDD2195  [6]
 LDCM0582  Fragment23 Ramos C116(0.62)  LDD2196  [6]
 LDCM0586  Fragment28 Ramos C116(0.86)  LDD2198  [6]
 LDCM0588  Fragment30 Ramos C116(0.56)  LDD2199  [6]
 LDCM0590  Fragment32 Ramos C116(1.17)  LDD2201  [6]
 LDCM0468  Fragment33 Ramos C116(0.54)  LDD2202  [6]
 LDCM0566  Fragment4 Ramos C160(1.00); C116(0.82)  LDD2184  [6]
 LDCM0569  Fragment7 Ramos C160(0.88); C116(1.78)  LDD2186  [6]
 LDCM0571  Fragment9 Ramos C116(0.56)  LDD2188  [6]
 LDCM0022  KB02 T cell-activated C116(5.46)  LDD1704  [2]
 LDCM0023  KB03 Ramos C160(0.75); C116(0.75)  LDD2183  [6]
 LDCM0024  KB05 Reh C160(0.94)  LDD3403  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Isovaleryl-CoA dehydrogenase, mitochondrial (IVD) Acyl-CoA dehydrogenase family P26440
Superoxide dismutase [Cu-Zn] (SOD1) Cu-Zn superoxide dismutase family P00441
Cytochrome c oxidase assembly factor 7 (COA7) Hcp beta-lactamase family Q96BR5
Prolyl 4-hydroxylase subunit alpha-3 (P4HA3) P4HA family Q7Z4N8
Inactive Ufm1-specific protease 1 (UFSP1) Peptidase C78 family Q6NVU6
E3 SUMO-protein ligase PIAS4 (PIAS4) PIAS family Q8N2W9
Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
14-3-3 protein zeta/delta (YWHAZ) 14-3-3 family P63104
Wolframin (WFS1) . O76024
Transcription factor
Click To Hide/Show 7 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Basic leucine zipper transcriptional factor ATF-like 2 (BATF2) BZIP family Q8N1L9
Zinc finger protein 20 (ZNF20) Krueppel C2H2-type zinc-finger protein family P17024
Nuclear receptor subfamily 0 group B member 2 (NR0B2) Nuclear hormone receptor family Q15466
Homeobox protein MOX-2 (MEOX2) . P50222
Transcriptional adapter 2-alpha (TADA2A) . O75478
Transcriptional repressor p66-beta (GATAD2B) . Q8WXI9
Zinc finger protein 581 (ZNF581) . Q9P0T4
Other
Click To Hide/Show 24 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ankyrin repeat and SOCS box protein 15 (ASB15) Ankyrin SOCS box (ASB) family Q8WXK1
Bcl-2-modifying factor (BMF) Bcl-2 family Q96LC9
Cancer/testis antigen 1 (CTAG1A; CTAG1B) CTAG/PCC1 family P78358
Guanine nucleotide-binding protein G(i) subunit alpha-1 (GNAI1) G-alpha family P63096
Guanine nucleotide-binding protein G(i) subunit alpha-2 (GNAI2) G-alpha family P04899
Guanine nucleotide-binding protein G(i) subunit alpha-3 (GNAI3) G-alpha family P08754
Progranulin (GRN) Granulin family P28799
Keratin-associated protein 19-4 (KRTAP19-4) KRTAP type 19 family Q3LI73
Methyl-CpG-binding domain protein 3-like 1 (MBD3L1) MBD3L family Q8WWY6
Harmonin-binding protein USHBP1 (USHBP1) MCC family Q8N6Y0
Protein Dr1 (DR1) NC2 beta/DR1 family Q01658
Keratin-associated protein 11-1 (KRTAP11-1) PMG family Q8IUC1
Keratin-associated protein 13-3 (KRTAP13-3) PMG family Q3SY46
R-spondin-4 (RSPO4) R-spondin family Q2I0M5
Heat shock protein beta-1 (HSPB1) Small heat shock protein (HSP20) family P04792
Protein sprouty homolog 3 (SPRY3) Sprouty family O43610
Syntaxin-binding protein 5-like (STXBP5L) WD repeat L(2)GL family Q9Y2K9
General transcription factor 3C polypeptide 3 (GTF3C3) . Q9Y5Q9
Heat shock factor 2-binding protein (HSF2BP) . O75031
IQ domain-containing protein E (IQCE) . Q6IPM2
Putative HIG1 domain family member 2B (HIGD2B) . Q4VC39
RING finger protein 11 (RNF11) . Q9Y3C5
TAR DNA-binding protein 43 (TARDBP) . Q13148
Uncharacterized protein KCNJ5-AS1 (KCNJ5-AS1) . Q8TAV5

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
3 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
5 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
6 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578