Details of the Target

General Information of Target

Target ID LDTP14029
Target Name WD repeat domain phosphoinositide-interacting protein 4 (WDR45)
Gene Name WDR45
Gene ID 11152
Synonyms
WDRX1; WDRXI4; WIPI4; WD repeat domain phosphoinositide-interacting protein 4; WIPI-4; WD repeat-containing protein 45
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MRDSTGAGNSLVHKRSPLRRNQKTPTSLTKLSLQDGHKAKKPACKFEEGQDVLARWSDGL
FYLGTIKKINILKQSCFIIFEDSSKSWVLWKDIQTGATGSGEMVCTICQEEYSEAPNEMV
ICDKCGQGYHQLCHTPHIDSSVIDSDEKWLCRQCVFATTTKRGGALKKGPNAKALQVMKQ
TLPYSVADLEWDAGHKTNVQQCYCYCGGPGDWYLKMLQCCKCKQWFHEACVQCLQKPMLF
GDRFYTFICSVCSSGPEYLKRLPLQWVDIAHLCLYNLSVIHKKKYFDSELELMTYINENW
DRLHPGELADTPKSERYEHVLEALNDYKTMFMSGKEIKKKKHLFGLRIRVPPVPPNVAFK
AEKEPEGTSHEFKIKGRKASKPISDSREVSNGIEKKGKKKSVGRPPGPYTRKMIQKTAEP
LLDKESISENPTLDLPCSIGRTEGTAHSSNTSDVDFTGASSAKETTSSSISRHYGLSDSR
KRTRTGRSWPAAIPHLRRRRGRLPRRALQTQNSEIVKDDEGKEDYQFDELNTEILNNLAD
QELQLNHLKNSITSYFGAAGRIACGEKYRVLARRVTLDGKVQYLVEWEGATAS
Target Bioclass
Other
Family
WD repeat PROPPIN family
Subcellular location
Preautophagosomal structure
Function
Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Binds phosphatidylinositol 3-phosphate (PtdIns3P). Activated by the STK11/AMPK signaling pathway upon starvation, WDR45 is involved in autophagosome assembly downstream of WIPI2, regulating the size of forming autophagosomes. Together with WIPI1, promotes ATG2 (ATG2A or ATG2B)-mediated lipid transfer by enhancing ATG2-association with phosphatidylinositol 3-monophosphate (PI3P)-containing membranes. Probably recruited to membranes through its PtdIns3P activity.
Uniprot ID
Q9Y484
Ensemble ID
ENST00000322995.13
HGNC ID
HGNC:28912

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Acrolein
 Probe Info  		N.A.  LDD0222  [1]
DBIA
 Probe Info  		C166(0.90)  LDD1571  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [1]
 LDCM0367  CL1 HEK-293T C166(0.90)  LDD1571  [2]
 LDCM0370  CL101 HEK-293T C166(0.95)  LDD1574  [2]
 LDCM0374  CL105 HEK-293T C166(0.84)  LDD1578  [2]
 LDCM0378  CL109 HEK-293T C166(0.81)  LDD1582  [2]
 LDCM0383  CL113 HEK-293T C166(0.99)  LDD1587  [2]
 LDCM0387  CL117 HEK-293T C166(0.91)  LDD1591  [2]
 LDCM0392  CL121 HEK-293T C166(0.97)  LDD1596  [2]
 LDCM0396  CL125 HEK-293T C166(1.05)  LDD1600  [2]
 LDCM0400  CL13 HEK-293T C166(0.87)  LDD1604  [2]
 LDCM0413  CL25 HEK-293T C166(0.95)  LDD1617  [2]
 LDCM0426  CL37 HEK-293T C166(0.88)  LDD1630  [2]
 LDCM0439  CL49 HEK-293T C166(0.94)  LDD1643  [2]
 LDCM0453  CL61 HEK-293T C166(0.91)  LDD1656  [2]
 LDCM0466  CL73 HEK-293T C166(0.85)  LDD1669  [2]
 LDCM0479  CL85 HEK-293T C166(0.90)  LDD1682  [2]
 LDCM0492  CL97 HEK-293T C166(0.93)  LDD1695  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Autophagy-related protein 2 homolog A (ATG2A) ATG2 family Q2TAZ0

References

1 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402