Details of the Target

General Information of Target

Target ID LDTP13939
Target Name N-acetylglucosamine-6-phosphate deacetylase (AMDHD2)
Gene Name AMDHD2
Gene ID 51005
Synonyms
N-acetylglucosamine-6-phosphate deacetylase; GlcNAc 6-P deacetylase; EC 3.5.1.25; Amidohydrolase domain-containing protein 2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAARLVSRCGAVRAAPHSGPLVSWRRWSGASTDTVYDVVVSGGGLVGAAMACALGYDIHF
HDKKILLLEAGPKKVLEKLSETYSNRVSSISPGSATLLSSFGAWDHICNMRYRAFRRMQV
WDACSEALIMFDKDNLDDMGYIVENDVIMHALTKQLEAVSDRVTVLYRSKAIRYTWPCPF
PMADSSPWVHITLGDGSTFQTKLLIGADGHNSGVRQAVGIQNVSWNYDQSAVVATLHLSE
ATENNVAWQRFLPSGPIALLPLSDTLSSLVWSTSHEHAAELVSMDEEKFVDAVNSAFWSD
ADHTDFIDTAGAMLQYAVSLLKPTKVSARQLPPSVARVDAKSRVLFPLGLGHAAEYVRPR
VALIGDAAHRVHPLAGQGVNMGFGDISSLAHHLSTAAFNGKDLGSVSHLTGYETERQRHN
TALLAATDLLKRLYSTSASPLVLLRTWGLQATNAVSPLKEQIMAFASK
Target Bioclass
Enzyme
Family
Metallo-dependent hydrolases superfamily, NagA family
Function
Hydrolyzes the N-glycolyl group from N-glycolylglucosamine 6-phosphate (GlcNGc-6-P) in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway. Although human is not able to catalyze formation of Neu5Gc due to the inactive CMAHP enzyme, Neu5Gc is present in food and must be degraded.
Uniprot ID
Q9Y303
Ensemble ID
ENST00000293971.11
HGNC ID
HGNC:24262
ChEMBL ID
CHEMBL3217376

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAL51 SNV: p.G176R DBIA    Probe Info 
CHL1 Insertion: p.A404GfsTer15 .
DANG SNV: p.S352T DBIA    Probe Info 
Ishikawa (Heraklio) 02 ER SNV: p.K25E .
JURKAT SNV: p.A281T .
MELJUSO Insertion: p.Q403_A404insGGG DBIA    Probe Info 
MEWO SNV: p.L138F .
MOLT4 SNV: p.G139W .
NCIH196 Deletion: p.Q82_A83del .
PF382 SNV: p.Y264C .
SSP25 SNV: p.T346I; p.Q392Ter .
SUIT2 SNV: p.V94M .
UMUC3 SNV: p.S129T .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C261(1.45)  LDD3311  [1]
4-Iodoacetamidophenylacetylene
 Probe Info  		C338(0.00); C18(0.00); C261(0.00)  LDD0038  [2]
IA-alkyne
 Probe Info  		C261(0.00); C206(0.00); C348(0.00); C338(0.00)  LDD0036  [2]
Lodoacetamide azide
 Probe Info  		C261(0.00); C206(0.00); C348(0.00); C338(0.00)  LDD0037  [2]
NAIA_5
 Probe Info  		C261(0.00); C206(0.00); C348(0.00); C338(0.00)  LDD2223  [3]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STS-1
 Probe Info  		N.A.  LDD0136  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 697 C261(1.13)  LDD2245  [1]
 LDCM0023  KB03 769-P C261(1.22)  LDD2663  [1]
 LDCM0024  KB05 G361 C261(1.45)  LDD3311  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Pachytene checkpoint protein 2 homolog (TRIP13) AAA ATPase family Q15645
Glucosamine-6-phosphate isomerase 2 (GNPDA2) Glucosamine/galactosamine-6-phosphate isomerase family Q8TDQ7
Caspase-6 (CASP6) Peptidase C14A family P55212
GTP-binding nuclear protein Ran (RAN) Ran family P62826
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Huntingtin-interacting protein 1 (HIP1) SLA2 family O00291

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
3 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
4 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.