Details of the Target

General Information of Target

Target ID LDTP13903
Target Name Tyrosine-protein phosphatase non-receptor type 22 (PTPN22)
Gene Name PTPN22
Gene ID 26191
Synonyms
PTPN8; Tyrosine-protein phosphatase non-receptor type 22; EC 3.1.3.48; Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP; Lymphoid phosphatase; LyP; PEST-domain phosphatase; PEP
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MASSGAGDPLDSKRGEAPFAQRIDPTREKLTPEQLHSMRQAELAQWQKVLPRRRTRNIVT
GLGIGALVLAIYGYTFYSISQERFLDELEDEAKAARARALARASGS
Target Bioclass
Enzyme
Family
Protein-tyrosine phosphatase family, Non-receptor class 4 subfamily
Subcellular location
Cytoplasm
Function
Acts as a negative regulator of T-cell receptor (TCR) signaling by direct dephosphorylation of the Src family kinases LCK and FYN, ITAMs of the TCRz/CD3 complex, as well as ZAP70, VAV, VCP and other key signaling molecules. Associates with and probably dephosphorylates CBL. Dephosphorylates LCK at its activating 'Tyr-394' residue. Dephosphorylates ZAP70 at its activating 'Tyr-493' residue. Dephosphorylates the immune system activator SKAP2. Positively regulates toll-like receptor (TLR)-induced type 1 interferon production. Promotes host antiviral responses mediated by type 1 interferon. Regulates NOD2-induced pro-inflammatory cytokine secretion and autophagy. Acts as an activator of NLRP3 inflammasome assembly by mediating dephosphorylation of 'Tyr-861' of NLRP3. Dephosphorylates phospho-anandamide (p-AEA), an endocannabinoid to anandamide (also called N-arachidonoylethanolamide).
Uniprot ID
Q9Y2R2
Ensemble ID
ENST00000460620.5
HGNC ID
HGNC:9652
ChEMBL ID
CHEMBL2889

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IA-alkyne
 Probe Info  		C763(20.00)  LDD1706  [1]
SF
 Probe Info  		N.A.  LDD0028  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0573  Fragment11 Ramos C425(0.17)  LDD2190  [3]
 LDCM0576  Fragment14 Ramos C425(0.90)  LDD2193  [3]
 LDCM0586  Fragment28 Ramos C425(1.84)  LDD2198  [3]
 LDCM0566  Fragment4 Ramos C425(1.57)  LDD2184  [3]
 LDCM0569  Fragment7 Ramos C425(0.74)  LDD2186  [3]
 LDCM0022  KB02 Ramos C425(1.06)  LDD2182  [3]
 LDCM0023  KB03 Ramos C425(1.04)  LDD2183  [3]
 LDCM0024  KB05 T cell-activated C763(20.00)  LDD1706  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Epidermal growth factor receptor (EGFR) Tyr protein kinase family P00533
Tyrosine-protein kinase Lck (LCK) Tyr protein kinase family P06239
Tyrosine-protein kinase ZAP-70 (ZAP70) Tyr protein kinase family P43403
TNF receptor-associated factor 3 (TRAF3) TNF receptor-associated factor family Q13114
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
T-cell surface glycoprotein CD3 zeta chain (CD247) CD3Z/FCER1G family P20963
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Growth factor receptor-bound protein 2 (GRB2) GRB2/sem-5/DRK family P62993
Src kinase-associated phosphoprotein 2 (SKAP2) SKAP family O75563
Proline-serine-threonine phosphatase-interacting protein 1 (PSTPIP1) . O43586

References

1 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
2 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
3 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578