Details of the Target

General Information of Target

Target ID LDTP13876
Target Name Rabphilin-3A (RPH3A)
Gene Name RPH3A
Gene ID 22895
Synonyms
KIAA0985; Rabphilin-3A; Exophilin-1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDVLPTGGGRPGLRTELEFRGGGGEARLESQEEETIPAAPPAPRLRGAAERPRRSRDTWD
GDEDTEPGEACGGRTSRTASLVSGLLNELYSCTEEEEAAGGGRGAEGRRRRRDSLDSSTE
ASGSDVVLGGRSGAGDSRVLQELQERPSQRHQMLYLRQKDANELKTILRELKYRIGIQSA
KLLRHLKQKDRLLHKVQRNCDIVTACLQAVSQKRRVDTKLKFTLEPSLGQNGFQQWYDAL
KAVARLSTGIPKEWRRKVWLTLADHYLHSIAIDWDKTMRFTFNERSNPDDDSMGIQIVKD
LHRTGCSSYCGQEAEQDRVVLKRVLLAYARWNKTVGYCQGFNILAALILEVMEGNEGDAL
KIMIYLIDKVLPESYFVNNLRALSVDMAVFRDLLRMKLPELSQHLDTLQRTANKESGGGY
EPPLTNVFTMQWFLTLFATCLPNQTVLKIWDSVFFEGSEIILRVSLAIWAKLGEQIECCE
TADEFYSTMGRLTQEMLENDLLQSHELMQTVYSMAPFPFPQLAELREKYTYNITPFPATV
KPTSVSGRHSKARDSDEENDPDDEDAVVNAVGCLGPFSGFLAPELQKYQKQIKEPNEEQS
LRSNNIAELSPGAINSCRSEYHAAFNSMMMERMTTDINALKRQYSRIKKKQQQQVHQVYI
RADKGPVTSILPSQVNSSPVINHLLLGKKMKMTNRAAKNAVIHIPGHTGGKISPVPYEDL
KTKLNSPWRTHIRVHKKNMPRTKSHPGCGDTVGLIDEQNEASKTNGLGAAEAFPSGCTAT
AGREGSSPEGSTRRTIEGQSPEPVFGDADVDVSAVQAKLGALELNQRDAAAETELRVHPP
CQRHCPEPPSAPEENKATSKAPQGSNSKTPIFSPFPSVKPLRKSATARNLGLYGPTERTP
TVHFPQMSRSFSKPGGGNSGTKKR
Target Type
Literature-reported
Target Bioclass
Other
Subcellular location
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane
Function
Plays an essential role in docking and fusion steps of regulated exocytosis. At the presynaptic level, RPH3A is recruited by RAB3A to the synaptic vesicle membrane in a GTP-dependent manner where it modulates synaptic vesicle trafficking and calcium-triggered neurotransmitter release. In the post-synaptic compartment, forms a ternary complex with GRIN2A and DLG4 and regulates NMDA receptor stability. Also plays a role in the exocytosis of arginine vasopressin hormone.
TTD ID
T95922
Uniprot ID
Q9Y2J0
DrugMap ID
TT9L4J8
Ensemble ID
ENST00000389385.9
HGNC ID
HGNC:17056

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 Deletion: p.I658SfsTer8
SNV: p.G337W		 .
A2058 SNV: p.P212S DBIA    Probe Info 
COLO792 SNV: p.D581N DBIA    Probe Info 
FUOV1 SNV: p.H466Y .
KMCH1 SNV: p.R133C .
KMS11 SNV: p.G308R .
MCAS SNV: p.H466P .
MCC13 SNV: p.G418S .
MFE319 Deletion: p.N674IfsTer6
SNV: p.P322H		 .
MOLM16 SNV: p.N648S .
NALM6 SNV: p.R573H .
OCUM1 SNV: p.E145D .
OVISE SNV: p.H466Y .
RKO SNV: p.S206T .
RL952 SNV: p.M170V .
SG231 SNV: p.D3E .
SKMEL30 SNV: p.W150Ter .
TE1 SNV: p.A579V .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IA-alkyne
 Probe Info  		C118(1.26)  LDD2157  [1]
DBIA
 Probe Info  		C126(1.34)  LDD3362  [2]
TFBX
 Probe Info  		N.A.  LDD0148  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 A2058 C140(1.53)  LDD2253  [2]
 LDCM0023  KB03 A2058 C140(1.51)  LDD2670  [2]
 LDCM0024  KB05 NCI-H446 C126(1.34)  LDD3362  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Peripheral plasma membrane protein CASK (CASK) CAMK Ser/Thr protein kinase family O14936
Ras-related protein Rab-27B (RAB27B) Rab family O00194

References

1 From chemoproteomic-detected amino acids to genomic coordinates: insights into precise multi-omic data integration. Mol Syst Biol. 2021 Feb;17(2):e9840. doi: 10.15252/msb.20209840.
Mass spectrometry data entry: PXD022151
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255