Details of the Target

General Information of Target

Target ID LDTP13707
Target Name E3 ubiquitin-protein ligase TRIM35 (TRIM35)
Gene Name TRIM35
Gene ID 23087
Synonyms
HLS5; KIAA1098; E3 ubiquitin-protein ligase TRIM35; EC 2.3.2.27; Hemopoietic lineage switch protein 5
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MNYQQQLANSAAIRAEIQRFESVHPNIYSIYELLERVEEPVLQNQIREHVIAIEDAFVNS
QEWTLSRSVPELKVGIVGNLASGKSALVHRYLTGTYVQEESPEGGRFKKEIVVDGQSYLL
LIRDEGGPPEAQFAMWVDAVIFVFSLEDEISFQTVYHYYSRMANYRNTSEIPLVLVGTQD
AISSANPRVIDDARARKLSNDLKRCTYYETCATYGLNVERVFQDVAQKIVATRKKQQLSI
GPCKSLPNSPSHSSVCSAQVSAVHISQTSNGGGSLSDYSSSVPSTPSTSQKELRIDVPPT
ANTPTPVRKQSKRRSNLFTSRKGSDPDKEKKGLESRADSIGSGRAIPIKQGMLLKRSGKS
LNKEWKKKYVTLCDNGVLTYHPSLHDYMQNVHGKEIDLLRTTVKVPGKRPPRATSACAPI
SSPKTNGLSKDMSSLHISPNSGNVTSASGSQMASGISLVSFNSRPDGMHQRSYSVSSADQ
WSEATVIANSAISSDTGLGDSVCSSPSISSTTSPKLDPPPSPHANRKKHRRKKSTSNFKA
DGLSGTAEEQEENFEFIIVSLTGQTWHFEATTYEERDAWVQAIESQILASLQSCESSKNK
SRLTSQSEAMALQSIRNMRGNSHCVDCETQNPNWASLNLGALMCIECSGIHRNLGTHLSR
VRSLDLDDWPVELIKVMSSIGNELANSVWEESSQGRTKPSVDSTREEKERWIRAKYEQKL
FLAPLPCTELSLGQHLLRATADEDLRTAILLLAHGSRDEVNETCGEGDGRTALHLACRKG
NVVLAQLLIWYGVDVTARDAHGNTALAYARQASSQECIDVLLQYGCPDERFVLMATPNLS
RRNNNRNNSSGRVPTII
Target Bioclass
Enzyme
Family
TRIM/RBCC family
Subcellular location
Cytoplasm
Function
E3 ubiquitin-protein ligase that participates in multiple biological processes including cell death, glucose metabolism, and in particular, the innate immune response. Mediates 'Lys-63'-linked polyubiquitination of TRAF3 thereby promoting type I interferon production via RIG-I signaling pathway. Can also catalyze 'Lys-48'-linked polyubiquitination and proteasomal degradation of viral proteins such as influenza virus PB2. Acts as a negative feedback regulator of TLR7- and TLR9-triggered signaling. Mechanistically, promotes the 'Lys-48'-linked ubiquitination of IRF7 and induces its degradation via a proteasome-dependent pathway. Reduces FGFR1-dependent tyrosine phosphorylation of PKM, inhibiting PKM-dependent lactate production, glucose metabolism, and cell growth.
Uniprot ID
Q9UPQ4
Ensemble ID
ENST00000305364.9
HGNC ID
HGNC:16285

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
DEL SNV: p.R35H .
HCT116 Insertion: p.L365AfsTer18 .
HCT15 SNV: p.G313D .
HEC1 SNV: p.R461H .
LN18 SNV: p.D6E .
NCIN87 SNV: p.P482R .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IPM
 Probe Info  		N.A.  LDD2156  [1]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 8 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
5'-AMP-activated protein kinase subunit beta-2 (PRKAB2) 5'-AMP-activated protein kinase beta subunit family O43741
Glycine N-methyltransferase (GNMT) Glycine N-methyltransferase family Q14749
Glutamine--tRNA ligase (QARS1) Class-I aminoacyl-tRNA synthetase family P47897
Probable E3 ubiquitin-protein ligase DTX2 (DTX2) Deltex family Q86UW9
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform (PPP2CA) PPP phosphatase family P67775
Testis-specific serine/threonine-protein kinase 3 (TSSK3) CAMK Ser/Thr protein kinase family Q96PN8
Fibroblast growth factor receptor 3 (FGFR3) Tyr protein kinase family P22607
Ubiquitin-conjugating enzyme E2 K (UBE2K) Ubiquitin-conjugating enzyme family P61086
Transporter and channel
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Glutamate receptor ionotropic, NMDA 2C (GRIN2C) Glutamate-gated ion channel family Q14957
Huntingtin (HTT) Huntingtin family P42858
Adiponectin (ADIPOQ) . Q15848
Wolframin (WFS1) . O76024
Other
Click To Hide/Show 14 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Progranulin (GRN) Granulin family P28799
Partitioning defective 6 homolog beta (PARD6B) PAR6 family Q9BYG5
Programmed cell death protein 5 (PDCD5) PDCD5 family O14737
Proline-rich protein 20D (PRR20D) PRR20 family P86480
Calcium-binding protein 2 (CABP2) . Q9NPB3
Collagen alpha-1(VIII) chain (COL8A1) . P27658
Enkurin domain-containing protein 1 (ENKD1) . Q9H0I2
KN motif and ankyrin repeat domain-containing protein 2 (KANK2) . Q63ZY3
PHD finger protein 24 (PHF24) . Q9UPV7
Rhombotin-1 (LMO1) . P25800
RING finger protein 11 (RNF11) . Q9Y3C5
SHC-transforming protein 3 (SHC3) . Q92529
Sprouty-related, EVH1 domain-containing protein 1 (SPRED1) . Q7Z699
Ubiquilin-1 (UBQLN1) . Q9UMX0

References

1 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
2 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004