Details of the Target

General Information of Target

Target ID LDTP13506
Target Name Sarcosine dehydrogenase, mitochondrial (SARDH)
Gene Name SARDH
Gene ID 1757
Synonyms
DMGDHL1; Sarcosine dehydrogenase, mitochondrial; SarDH; EC 1.5.8.3; BPR-2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MPILLFLIDTSASMNQRSHLGTTYLDTAKGAVETFMKLRARDPASRGDRYMLVTFEEPPY
AIKAGWKENHATFMNELKNLQAEGLTTLGQSLRTAFDLLNLNRLVTGIDNYGQGRNPFFL
EPAIIITITDGSKLTTTSGVQDELHLPLNSPLPGSELTKEPFRWDQRLFALVLRLPGTMS
VESEQLTGVPLDDSAITPMCEVTGGRSYSVCSPRMLNQCLESLVQKVQSGVVINFEKAGP
DPSPVEDGQPDISRPFGSQPWHSCHKLIYVRPNPKTGVPIGHWPVPESFWPDQNSPTLPP
RTSHPVVKFSCTDCEPMVIDKLPFDKYELEPSPLTQFILERKSPQTCWQVYVSNSAKYSE
LGHPFGYLKASTALNCVNLFVMPYNYPVLLPLLDDLFKVHKAKPTLKWRQSFESYLKTMP
PYYLGPLKKAVRMMGAPNLIADSMEYGLSYSVISYLKKLSQQAKIESDRVIGSVGKKVVQ
ETGIKVRSRSHGLSMAYRKDFQQLLQGISEDVPHRLLDLNMKEYTGFQVALLNKDLKPQT
FRNAYDIPRRNLLDHLTRMRSNLLKSTRRFLKGQDEDQVHSVPIAQMGNYQEYLKQVPSP
LRELDPDQPRRLHTFGNPFKLDKKGMMIDEADEFVAGPQNKHKRPGEPNMQGIPKRRRCM
SPLLRGRQQNPVVNNHIGGKGPPAPTTQAQPDLIKPLPLHKISETTNDSIIHDVVENHVA
DQLSSDITPNAMDTEFSASSPASLLERPTNHMEALGHDHLGTNDLTVGGFLENHEEPRDK
EQCAEENIPASSLNKGKKLMHCRSHEEVNTELKAQIMKEIRKPGRKYERIFTLLKHVQGS
LQTRLIFLQNVIKEASRFKKRMLIEQLENFLDEIHRRANQINHINSN
Target Bioclass
Enzyme
Family
GcvT family
Subcellular location
Mitochondrion matrix
Function Catalyzes the last step of the oxidative degradation of choline to glycine. Converts sarcosine into glycine.
Uniprot ID
Q9UL12
Ensemble ID
ENST00000371872.8
HGNC ID
HGNC:10536

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A2058 SNV: p.R241C .
ABC1 SNV: p.G323A .
CHL1 SNV: p.F403L .
CORL23 SNV: p.L220I .
CORL88 SNV: p.T547A .
DU145 SNV: p.A818V .
HCC15 SNV: p.G272Ter .
HCT15 SNV: p.T52I .
HEC1 Deletion: p.Q422RfsTer62 DBIA    Probe Info 
HEC1B Deletion: p.Q422RfsTer62 .
IGROV1 SNV: p.G215S .
IM95 Deletion: p.T145RfsTer38
SNV: p.C217R		 .
IPC298 SNV: p.P790L DBIA    Probe Info 
KMS27 SNV: p.A782E DBIA    Probe Info 
MFE319 SNV: p.R507Ter DBIA    Probe Info 
NCIH1944 SNV: p.Y204Ter .
NUGC3 SNV: p.L125F .
OZ Substitution: p.S65I .
SW403 SNV: p.P602S .
TOV21G SNV: p.F550L .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IPM
 Probe Info  		C497(0.00); C799(0.00); C235(0.00)  LDD0241  [1]
DBIA
 Probe Info  		C744(7.86)  LDD0209  [2]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [3]
NAIA_4
 Probe Info  		N.A.  LDD2226  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 786-O C827(2.90)  LDD2247  [5]
 LDCM0023  KB03 Jurkat C744(7.86)  LDD0209  [2]
 LDCM0024  KB05 MV4-11 C67(1.14)  LDD3338  [5]

References

1 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
2 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
5 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840