Details of the Target

General Information of Target

Target ID LDTP13298
Target Name Inactive cell surface hyaluronidase CEMIP2 (CEMIP2)
Gene Name CEMIP2
Gene ID 23670
Synonyms
KIAA1412; TMEM2; Inactive cell surface hyaluronidase CEMIP2; Cell migration-inducing hyaluronidase 2; Transmembrane protein 2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MRSRVAVRACHKVCRCLLSGFGGRVDAGQPELLTERSSPKGGHVKSHAELEGNGEHPEAP
GSGEGSEALLEICQRRHFLSGSKQQLSRDSLLSGCHPGFGPLGVELRKNLAAEWWTSVVV
FREQVFPVDALHHKPGPLLPGDSAFRLVSAETLREILQDKELSKEQLVAFLENVLKTSGK
LRENLLHGALEHYVNCLDLVNKRLPYGLAQIGVCFHPVFDTKQIRNGVKSIGEKTEASLV
WFTPPRTSNQWLDFWLRHRLQWWRKFAMSPSNFSSSDCQDEEGRKGNKLYYNFPWGKELI
ETLWNLGDHELLHMYPGNVSKLHGRDGRKNVVPCVLSVNGDLDRGMLAYLYDSFQLTENS
FTRKKNLHRKVLKLHPCLAPIKVALDVGRGPTLELRQVCQGLFNELLENGISVWPGYLET
MQSSLEQLYSKYDEMSILFTVLVTETTLENGLIHLRSRDTTMKEMMHISKLKDFLIKYIS
SAKNV
Target Bioclass
Other
Family
CEMIP family
Subcellular location
Cell membrane
Function
Unlike its mouse ortholog has no catalytic hyaluronic acid-degrading activity, but acts as a regulator of hyaluronan (HA) metabolism through regulation of expression of CEMIP and HAS2, two enzymes involved in HA depolymerization and HA synthesis, respectively.
Uniprot ID
Q9UHN6
Ensemble ID
ENST00000377044.9
HGNC ID
HGNC:11869

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 SNV: p.D556G; p.I1353T .
HEC1 SNV: p.R333W .
HEC1B SNV: p.R333W .
HL60 SNV: p.N914S .
HT115 SNV: p.N525T; p.E820Ter .
HUH7 SNV: p.P768Q DBIA    Probe Info 
Ishikawa (Heraklio) 02 ER Substitution: p.K77_R78delinsNTer .
KYSE150 SNV: p.A87P .
MELJUSO SNV: p.S249F DBIA    Probe Info 
MM1S SNV: p.S397N .
NCIH1793 SNV: p.N1018S .
NCIH2172 SNV: p.E1359Ter .
RKO SNV: p.R54Ter .
SNGM Substitution: p.F254V .
SNU308 SNV: p.A1030G .
T98G SNV: p.R302T .
TOV21G SNV: p.P1363L .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
YN-1
 Probe Info  		100.00  LDD0444  [1]
STPyne
 Probe Info  		K1262(10.00); K1377(5.57); K733(10.00)  LDD0277  [2]
OPA-S-S-alkyne
 Probe Info  		K856(0.77); K887(1.21); K1278(1.31); K846(1.51)  LDD3494  [3]
HHS-475
 Probe Info  		Y847(0.23)  LDD0268  [4]
DBIA
 Probe Info  		C1192(1.09); C1195(1.09)  LDD0078  [5]
IA-alkyne
 Probe Info  		N.A.  LDD0162  [6]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0020  ARS-1620 HCC44 C1192(1.09); C1195(1.09)  LDD0078  [5]
 LDCM0371  CL102 HEK-293T C1253(0.89)  LDD1575  [7]
 LDCM0375  CL106 HEK-293T C1253(0.76)  LDD1579  [7]
 LDCM0380  CL110 HEK-293T C1253(0.79)  LDD1584  [7]
 LDCM0384  CL114 HEK-293T C1253(0.98)  LDD1588  [7]
 LDCM0388  CL118 HEK-293T C1253(0.87)  LDD1592  [7]
 LDCM0393  CL122 HEK-293T C1253(0.92)  LDD1597  [7]
 LDCM0397  CL126 HEK-293T C1253(0.96)  LDD1601  [7]
 LDCM0401  CL14 HEK-293T C1253(1.07)  LDD1605  [7]
 LDCM0407  CL2 HEK-293T C1253(0.71)  LDD1611  [7]
 LDCM0414  CL26 HEK-293T C1253(1.03)  LDD1618  [7]
 LDCM0441  CL50 HEK-293T C1253(1.03)  LDD1645  [7]
 LDCM0454  CL62 HEK-293T C1253(1.03)  LDD1657  [7]
 LDCM0467  CL74 HEK-293T C1253(0.86)  LDD1670  [7]
 LDCM0480  CL86 HEK-293T C1253(1.04)  LDD1683  [7]
 LDCM0493  CL98 HEK-293T C1253(0.91)  LDD1696  [7]
 LDCM0427  Fragment51 HEK-293T C1253(0.76)  LDD1631  [7]
 LDCM0120  HHS-0701 DM93 Y847(0.23)  LDD0268  [4]
 LDCM0022  KB02 A2058 C554(1.70)  LDD2253  [8]
 LDCM0023  KB03 A2058 C750(2.47); C554(2.13)  LDD2670  [8]
 LDCM0024  KB05 HMCB C750(1.98); C554(1.35)  LDD3312  [8]

The Interaction Atlas With This Target

The Drug(s) Related To This Target

Approved
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Hyaluronic Acid Small molecular drug DB08818

References

1 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
4 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
5 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
6 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
7 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
8 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840