Details of the Target

General Information of Target

Target ID LDTP13292
Target Name Alpha-methylacyl-CoA racemase (AMACR)
Gene Name AMACR
Gene ID 23600
Synonyms
Alpha-methylacyl-CoA racemase; EC 5.1.99.4; 2-methylacyl-CoA racemase
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAEPTSDFETPIGWHASPELTPTLGPLSDTAPPRDSWMFWAMLPPPPPPLTSSLPAAGSK
PSSESQPPMEAQSLPGAPPPFDAQILPGAQPPFDAQSPLDSQPQPSGQPWNFHASTSWYW
RQSSDRFPRHQKSFNPAVKNSYYPRKYDAKFTDFSLPPSRKQKKKKRKEPVFHFFCDTCD
RGFKNQEKYDKHMSEHTKCPELDCSFTAHEKIVQFHWRNMHAPGMKKIKLDTPEEIARWR
EERRKNYPTLANIERKKKLKLEKEKRGAVLTTTQYGKMKGMSRHSQMAKIRSPGKNHKWK
NDNSRQRAVTGSGSHLCDLKLEGPPEANADPLGVLINSDSESDKEEKPQHSVIPKEVTPA
LCSLMSSYGSLSGSESEPEETPIKTEADVLAENQVLDSSAPKSPSQDVKATVRNFSEAKS
ENRKKSFEKTNPKRKKDYHNYQTLFEPRTHHPYLLEMLLAPDIRHERNVILQCVRYIIKK
DFFGLDTNSAKSKDV
Target Type
Literature-reported
Target Bioclass
Enzyme
Family
CoA-transferase III family
Subcellular location
Peroxisome
Function
Catalyzes the interconversion of (R)- and (S)-stereoisomers of alpha-methyl-branched-chain fatty acyl-CoA esters. Acts only on coenzyme A thioesters, not on free fatty acids, and accepts as substrates a wide range of alpha-methylacyl-CoAs, including pristanoyl-CoA, trihydroxycoprostanoyl-CoA (an intermediate in bile acid synthesis), and arylpropionic acids like the anti-inflammatory drug ibuprofen (2-(4-isobutylphenyl)propionic acid) but neither 3-methyl-branched nor linear-chain acyl-CoAs.
TTD ID
T92768
Uniprot ID
Q9UHK6
DrugMap ID
TTLN1AP
Ensemble ID
ENST00000335606.11
HGNC ID
HGNC:451

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 SNV: p.Q4H .
MFE319 SNV: p.G289D .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K58(4.85); K87(8.33)  LDD0277  [1]
DBIA
 Probe Info  		C117(1.15)  LDD3313  [2]
Acrolein
 Probe Info  		N.A.  LDD0221  [3]
JW-RF-010
 Probe Info  		N.A.  LDD0026  [4]
TFBX
 Probe Info  		N.A.  LDD0027  [4]
IPM
 Probe Info  		N.A.  LDD0147  [4]
Crotonaldehyde
 Probe Info  		N.A.  LDD0219  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0107  IAA HeLa N.A.  LDD0221  [3]
 LDCM0022  KB02 A101D C117(2.39)  LDD2250  [2]
 LDCM0023  KB03 A101D C117(2.66)  LDD2667  [2]
 LDCM0024  KB05 Hs 936.T C117(1.15)  LDD3313  [2]

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
4 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255