Details of the Target

General Information of Target

Target ID LDTP13061
Target Name Kelch-like protein 42 (KLHL42)
Gene Name KLHL42
Gene ID 57542
Synonyms
KIAA1340; KLHDC5; Kelch-like protein 42; Cullin-3-binding protein 9; Ctb9; Kelch domain-containing protein 5
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MADANKAEVPGATGGDSPHLQPAEPPGEPRREPHPAEAEKQQPQHSSSSNGVKMENDESA
KEEKSDLKEKSTGSKKANRFHPYSKDKNSGAGEKKGPNRNRVFISNIPYDMKWQAIKDLM
REKVGEVTYVELFKDAEGKSRGCGVVEFKDEEFVKKALETMNKYDLSGRPLNIKEDPDGE
NARRALQRTGGSFPGGHVPDMGSGLMNLPPSILNNPNIPPEVISNLQAGRLGSTIFVANL
DFKVGWKKLKEVFSIAGTVKRADIKEDKDGKSRGMGTVTFEQAIEAVQAISMFNGQFLFD
RPMHVKMDDKSVPHEEYRSHDGKTPQLPRGLGGIGMGLGPGGQPISASQLNIGGVMGNLG
PGGMGMDGPGFGGMNRIGGGIGFGGLEAMNSMGGFGGVGRMGELYRGAMTSSMERDFGRG
DIGINQGFGDSFGRLGSAMIGGFAGRIGSSNMGPVGSGISGGMGSMNSVTGGMGMGLDRM
SSSFDRMGPGIGAILERSIDMDRGFLSGPMGSGMRERIGSKGNQIFVRNLPFDLTWQKLK
EKFSQCGHVMFAEIKMENGKSKGCGTVRFDSPESAEKACRIMNGIKISGREIDVRLDRNA
Target Bioclass
Other
Subcellular location
Cytoplasm
Function
Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for mitotic progression and cytokinesis. The BCR(KLHL42) E3 ubiquitin ligase complex mediates the ubiquitination and subsequent degradation of KATNA1. Involved in microtubule dynamics throughout mitosis.
Uniprot ID
Q9P2K6
Ensemble ID
ENST00000381271.7
HGNC ID
HGNC:29252

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C15(2.85)  LDD3331  [1]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [2]
TFBX
 Probe Info  		N.A.  LDD0148  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0371  CL102 HEK-293T C365(0.84)  LDD1575  [4]
 LDCM0375  CL106 HEK-293T C365(0.93)  LDD1579  [4]
 LDCM0380  CL110 HEK-293T C365(1.04)  LDD1584  [4]
 LDCM0384  CL114 HEK-293T C365(1.44)  LDD1588  [4]
 LDCM0388  CL118 HEK-293T C365(1.14)  LDD1592  [4]
 LDCM0393  CL122 HEK-293T C365(0.99)  LDD1597  [4]
 LDCM0397  CL126 HEK-293T C365(0.79)  LDD1601  [4]
 LDCM0401  CL14 HEK-293T C365(0.92)  LDD1605  [4]
 LDCM0407  CL2 HEK-293T C365(0.96)  LDD1611  [4]
 LDCM0414  CL26 HEK-293T C365(0.86)  LDD1618  [4]
 LDCM0441  CL50 HEK-293T C365(1.32)  LDD1645  [4]
 LDCM0454  CL62 HEK-293T C365(1.27)  LDD1657  [4]
 LDCM0467  CL74 HEK-293T C365(0.96)  LDD1670  [4]
 LDCM0480  CL86 HEK-293T C365(1.09)  LDD1683  [4]
 LDCM0493  CL98 HEK-293T C365(1.36)  LDD1696  [4]
 LDCM0427  Fragment51 HEK-293T C365(1.16)  LDD1631  [4]
 LDCM0022  KB02 A101D C15(1.65)  LDD2250  [1]
 LDCM0023  KB03 A204 C15(1.46)  LDD2669  [1]
 LDCM0024  KB05 MKN-1 C15(2.85)  LDD3331  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Creatine kinase S-type, mitochondrial (CKMT2) ATP:guanido phosphotransferase family P17540
Protein DDI1 homolog 1 (DDI1) DDI1 family Q8WTU0
Protein disulfide-isomerase (P4HB) Protein disulfide isomerase family P07237
E3 ubiquitin-protein ligase TRIM23 (TRIM23) Arf family P36406
Tripartite motif-containing protein 54 (TRIM54) . Q9BYV2
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Myogenin (MYOG) . P15173
Other
Click To Hide/Show 17 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Afadin- and alpha-actinin-binding protein (SSX2IP) ADIP family Q9Y2D8
Calcium-binding and coiled-coil domain-containing protein 2 (CALCOCO2) CALCOCO family Q13137
Protein FAM168A (FAM168A) FAM168 family Q92567
Protein INCA1 (INCA1) INCA family Q0VD86
Keratin-associated protein 19-4 (KRTAP19-4) KRTAP type 19 family Q3LI73
Protein Mis18-beta (OIP5) Mis18 family O43482
Pro-opiomelanocortin (POMC) POMC family P01189
Prefoldin subunit 5 (PFDN5) Prefoldin subunit alpha family Q99471
Signal transducing adapter molecule 2 (STAM2) STAM family O75886
Vacuolar protein sorting-associated protein 52 homolog (VPS52) VPS52 family Q8N1B4
Cancer/testis antigen 55 (CT55) . Q8WUE5
Cell division cycle-associated protein 4 (CDCA4) . Q9BXL8
DAZ-associated protein 2 (DAZAP2) . Q15038
SERTA domain-containing protein 1 (SERTAD1) . Q9UHV2
SERTA domain-containing protein 3 (SERTAD3) . Q9UJW9
Ubiquilin-1 (UBQLN1) . Q9UMX0
Ubiquilin-2 (UBQLN2) . Q9UHD9

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
3 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
4 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402