Details of the Target

General Information of Target

Target ID LDTP13014
Target Name Dynein axonemal heavy chain 2 (DNAH2)
Gene Name DNAH2
Gene ID 146754
Synonyms
DNAHC2; DNHD3; KIAA1503; Dynein axonemal heavy chain 2; Axonemal beta dynein heavy chain 2; Ciliary dynein heavy chain 2; Dynein heavy chain domain-containing protein 3
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDVTVSELLELFLQSPLVTWVKTFGPFGSGSQDNLTMYMDLVDGIFLNQIMLQIDPRPTN
QRINKHVNNDVNLRIQNLTILVRNIKTYYQEVLQQLIVMNLPNVLMIGRDPLSGKSMEEI
KKVLLLVLGCAVQCERKEEFIERIKQLDIETQAGIVAHIQEVTHNQENVFDLQWLELPDV
APEELEALSRSMVLHLRRLIDQRDECTELIVDLTQERDYLQAQHPPSPIKSSSADSTPSP
TSSLSSEDKQHLAVELADTKARLRRVRQELEDKTEQLVDTRHEVDQLVLELQKVKQENIQ
LAADARSARAYRDELDSLREKANRVERLELELTRCKEKLHDVDFYKARMEELREDNIILI
ETKAMLEEQLTAARARGDKVHELEKENLQLKSKLHDLELDRDTDKKRIEELLEENMVLEI
AQKQSMNESAHLGWELEQLSKNADLSDASRKSFVFELNECASSRILKLEKENQSLQSTIQ
GLRDASLVLEESGLKCGELEKENHQLSKKIEKLQTQLEREKQSNQDLETLSEELIREKEQ
LQSDMETLKADKARQIKDLEQEKDHLNRAMWSLRERSQVSSEARMKDVEKENKALHQTVT
EANGKLSQLEFEKRQLHRDLEQAKEKGERAEKLERELQRLQEENGRLARKVTSLETATEK
VEALEHESQGLQLENRTLRKSLDTLQNVSLQLEGLERDNKQLDAENLELRRLVETMRFTS
TKLAQMERENQQLEREKEELRKNVDLLKALGKKSERLELSYQSVSAENLRLQQSLESSSH
KTQTLESELGELEAERQALRRDLEALRLANAQLEGAEKDRKALEQEVAQLEKDKKLLEKE
AKRLWQQVELKDAVLDDSTAKLSAVEKESRALDKELARCRDAAGKLKELEKDNRDLTKQV
TVHARTLTTLREDLVLEKLKSQQLSSELDKLSQELEKVGLNRELLLQEDDSGSDTKYKIL
EGRNESALKTTLAMKEEKIVLLEAQMEEKASLNRQLESELQMLKKECETLRQNQGEGQHL
QNSFKHPAGKTAASHQGKEAWGPGHKEATMELLRVKDRAIELERNNAALQAEKQLLKEQL
QHLETQNVTFSSQILTLQKQSAFLQEHNTTLQTQTAKLQVENSTLSSQSAALTAQYTLLQ
NHHTAKETENESLQRQQEQLTAAYEALLQDHEHLGTLHERQSAEYEALIRQHSCLKTLHR
NLELEHKELGERHGDMLKRKAELEEREKVLTTEREALQQEQRTNALAMGENQRLRGELDR
VNFLHHQLKGEYEELHAHTKELKTSLNNAQLELNRWQARFDELKEQHQTMDISLTKLDNH
CELLSRLKGNLEEENHHLLSQIQLLSQQNQMLLEQNMENKEQYHEEQKQYIDKLNALRRH
KEKLEEKIMDQYKFYDPPPKKKNHWIGAKALVKLIKPKKEGSRERLKSTVDSPPWQLESS
DPASPAASQPLRSQAENPDTPALGSNCAEERDAHNGSVGKGPGDLKPKRGSPHRGSLDRT
DASTDLAMRSWPSELGSRTCSTSATTTAPSNSTPIARHPGRTKGYNSDDNLCEPSLEFEV
PNHRQYVSRPSSLESSRNTSSNSSPLNLKGSSEQLHGRSESFSSEDLIPSRDLATLPREA
STPGRNALGRHEYPLPRNGPLPQEGAQKRGTAPPYVGVRPCSASPSSEMVTLEEFLEESN
RSSPTHDTPSCRDDLLSDYFRKASDPPAIGGQPGPPAKKEGAKMPTNFVAPTVKMAAPTS
EGRPLKPGQYVKPNFRLTEAEAPPSVAPRQAQPPQSLSLGRPRQAPVPPASHAPASRSAS
LSRAFSLASADLLRASGPEACKQESPQKLGAPEALGGRETGSHTLQSPAPPSSHSLARER
TPLVGKAGSSCQGPGPRSRPLDTRRFSLAPPKEERLAPLHQSATAPAIATAGAGAAAAGS
GSNSQLLHFSPAAAPAARTKPKAPPRSGEVATITPVRAGLSLSEGDGVPGQGCSEGLPAK
SPGRSPDLAPHLGRALEDCSRGSVSKSSPASPEPGGDPQTVWYEYGCV
Target Bioclass
Other
Family
Dynein heavy chain family
Subcellular location
Cytoplasm, cytoskeleton, cilium axoneme
Function
As part of the axonemal inner dynein arm complex plays a central role in ciliary beat. Expressed in sperm flagellum, it is required for sperm motility. Dyneins are microtubule-based molecular motors possessing ATPase activities that can convert the chemical energy of ATP into relative sliding between adjacent microtubule doublets to generate ciliary bending.
Uniprot ID
Q9P225
Ensemble ID
ENST00000389173.6
HGNC ID
HGNC:2948

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 Deletion: p.L1781Ter
SNV: p.R976S		 .
AU565 SNV: p.E4369Q .
CAL51 SNV: p.M1180T .
CCK81 Deletion: p.K4326RfsTer84 .
COLO320 Deletion: p.L3763CfsTer28 .
COLO679 SNV: p.L2136F .
COLO792 SNV: p.G1266E .
DANG SNV: p.N3151T .
DU145 SNV: p.G2474V .
G402 SNV: p.C2154W .
GB1 SNV: p.E3696K .
HCT15 SNV: p.V1681G; p.V2018I; p.T2172I; p.L3617M .
HEC1 SNV: p.S2460I; p.D2860E .
HEC1B SNV: p.D2860E .
HEP3B217 SNV: p.L3518M .
HT115 SNV: p.R10Q; p.F3839L .
ICC3 SNV: p.R1985L .
IGROV1 SNV: p.G711Ter .
KYSE410 SNV: p.E1349K .
LNCaP clone FGC SNV: p.D119G .
LOXIMVI SNV: p.E258K .
LS180 SNV: p.D2897V .
MCC13 SNV: p.P224S .
MFE319 SNV: p.Y2291C .
NALM6 SNV: p.G328D; p.V1078M; p.M1413T; p.R2334W .
NCIH1666 SNV: p.A890S .
NCIH1944 SNV: p.P4273H .
NCIH196 SNV: p.Q4069K .
NCIH446 SNV: p.K1021M .
NUGC3 SNV: p.S1549P .
OCIAML2 SNV: p.L1068P .
PFSK1 SNV: p.T946S .
RCC10RGB SNV: p.T87R .
REH SNV: p.R483W .
RKO Deletion: p.K390RfsTer31
SNV: p.T1471A		 .
RPMI8226 SNV: p.V3021G .
SKMEL2 SNV: p.G1459R .
SNGM SNV: p.E1030Ter .
SNU1 SNV: p.Y3045C .
SUPT1 SNV: p.D3256G .
SW1116 SNV: p.V2396I .
TE4 SNV: p.A835T .
TF1 SNV: p.D813N .
THP1 SNV: p.L315S .
UACC257 SNV: p.E1261K .
UACC62 SNV: p.M2626R .
YSCCC SNV: p.F2257L .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IPM
 Probe Info  		N.A.  LDD0241  [2]
Acrolein
 Probe Info  		C1628(0.00); H1629(0.00)  LDD0221  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa C1628(0.00); H1629(0.00)  LDD0222  [3]
 LDCM0107  IAA HeLa C1628(0.00); H1629(0.00)  LDD0221  [3]
 LDCM0109  NEM HeLa C1628(0.00); H1629(0.00)  LDD0223  [3]

References

1 Labeling Preferences of Diazirines with Protein Biomolecules. J Am Chem Soc. 2021 May 5;143(17):6691-6700. doi: 10.1021/jacs.1c02509. Epub 2021 Apr 20.
Mass spectrometry data entry: PXD025140
2 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
3 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.