General Information of Target

Target ID LDTP12977
Target Name GSK3B-interacting protein (GSKIP)
Gene Name GSKIP
Gene ID 51527
Synonyms
C14orf129; GSK3B-interacting protein; GSKIP; GSK3beta interaction protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAMASVKLLAGVLRKPDAWIGLWGVLRGTPSSYKLCTSWNRYLYFSSTKLRAPNYKTLFY
NIFSLRLPGLLLSPECIFPFSVRLKSNIRSTKSTKKSLQKVDEEDSDEESHHDEMSEQEE
ELEDDPTVVKNYKDLEKAVQSFRYDVVLKTGLDIGRNKVEDAFYKGELRLNEEKLWKKSR
TVKVGDTLDLLIGEDKEAGTETVMRILLKKVFEEKTESEKYRVVLRRWKSLKLPKKRMSK
Target Bioclass
Other
Family
GSKIP family
Subcellular location
Cytoplasm
Function
A-kinase anchoring protein for GSK3B and PKA that regulates or facilitates their kinase activity towards their targets. The ternary complex enhances Wnt-induced signaling by facilitating the GSK3B- and PKA-induced phosphorylation of beta-catenin leading to beta-catenin degradation and stabilization respectively. Upon cAMP activation, the ternary complex contributes to neuroprotection against oxidative stress-induced apoptosis by facilitating the PKA-induced phosphorylation of DML1 and PKA-induced inactivation of GSK3B. During neurite outgrowth promotes neuron proliferation; while increases beta-catenin-induced transcriptional activity through GSK3B kinase activity inhibition, reduces N-cadherin level to promote cell cycle progression.
Uniprot ID
Q9P0R6
Ensemble ID
ENST00000438650.5
HGNC ID
HGNC:20343

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IA-alkyne
 Probe Info 
C59(5.10)  LDD2157  [1]
DBIA
 Probe Info 
C77(1.61)  LDD3325  [2]
m-APA
 Probe Info 
N.A.  LDD2232  [3]
NAIA_5
 Probe Info 
C59(0.00); C77(0.00)  LDD2223  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0281  AC21 HEK-293T C59(1.05)  LDD1520  [5]
 LDCM0289  AC29 HEK-293T C59(1.07)  LDD1528  [5]
 LDCM0298  AC37 HEK-293T C59(1.03)  LDD1537  [5]
 LDCM0307  AC45 HEK-293T C59(0.94)  LDD1546  [5]
 LDCM0312  AC5 HEK-293T C59(0.93)  LDD1551  [5]
 LDCM0316  AC53 HEK-293T C59(1.09)  LDD1555  [5]
 LDCM0325  AC61 HEK-293T C59(0.95)  LDD1564  [5]
 LDCM0248  AKOS034007472 HEK-293T C59(1.25)  LDD1511  [5]
 LDCM0632  CL-Sc Hep-G2 C59(20.00)  LDD2227  [4]
 LDCM0409  CL21 HEK-293T C59(1.03)  LDD1613  [5]
 LDCM0422  CL33 HEK-293T C59(0.96)  LDD1626  [5]
 LDCM0435  CL45 HEK-293T C59(0.97)  LDD1639  [5]
 LDCM0448  CL57 HEK-293T C59(1.72)  LDD1651  [5]
 LDCM0461  CL69 HEK-293T C59(1.06)  LDD1664  [5]
 LDCM0475  CL81 HEK-293T C59(1.43)  LDD1678  [5]
 LDCM0484  CL9 HEK-293T C59(0.94)  LDD1687  [5]
 LDCM0488  CL93 HEK-293T C59(1.16)  LDD1691  [5]
 LDCM0022  KB02 A-375 C77(1.48)  LDD2255  [2]
 LDCM0023  KB03 A-375 C77(1.76)  LDD2672  [2]
 LDCM0024  KB05 UACC257 C77(1.61)  LDD3325  [2]

References

1 From chemoproteomic-detected amino acids to genomic coordinates: insights into precise multi-omic data integration. Mol Syst Biol. 2021 Feb;17(2):e9840. doi: 10.15252/msb.20209840.
Mass spectrometry data entry: PXD022151
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402