Details of the Target

General Information of Target

Target ID LDTP12975
Target Name E3 ubiquitin-protein ligase RNF181 (RNF181)
Gene Name RNF181
Gene ID 51255
Synonyms
E3 ubiquitin-protein ligase RNF181; EC 2.3.2.27; RING finger protein 181
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MTEDSQRNFRSVYYEKVGFRGVEEKKSLEILLKDDRLDTEKLCTFSQRFPLPSMYRALVW
KVLLGILPPHHESHAKVMMYRKEQYLDVLHALKVVRFVSDATPQAEVYLRMYQLESGKLP
RSPSFPLEPDDEVFLAIAKAMEEMVEDSVDCYWITRRFVNQLNTKYRDSLPQLPKAFEQY
LNLEDGRLLTHLRMCSAAPKLPYDLWFKRCFAGCLPESSLQRVWDKVVSGSCKILVFVAV
EILLTFKIKVMALNSAEKITKFLENIPQDSSDAIVSKAIDLWHKHCGTPVHSS
Target Bioclass
Enzyme
Family
RNF181 family
Function
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Catalyzes monoubiquitination of 26S proteasome subunit PSMC2/RPT1.
Uniprot ID
Q9P0P0
Ensemble ID
ENST00000306368.9
HGNC ID
HGNC:28037

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K137(7.14)  LDD0277  [1]
BTD
 Probe Info  		C116(1.11)  LDD2111  [2]
IA-alkyne
 Probe Info  		N.A.  LDD0166  [3]
IPM
 Probe Info  		N.A.  LDD0025  [4]
NAIA_5
 Probe Info  		N.A.  LDD2224  [5]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [6]
W1
 Probe Info  		C113(0.00); C116(0.00)  LDD0236  [7]
AOyne
 Probe Info  		13.40  LDD0443  [8]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0632  CL-Sc Hep-G2 C10(1.07)  LDD2227  [5]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C116(1.11)  LDD2111  [2]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C116(0.89)  LDD2134  [2]
 LDCM0554  Nucleophilic fragment 7a MDA-MB-231 C116(0.50)  LDD2148  [2]
 LDCM0627  NUDT7-COV-1 HEK-293T C10(0.84)  LDD2206  [9]
 LDCM0628  OTUB2-COV-1 HEK-293T C10(0.99)  LDD2207  [9]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ubiquitin-conjugating enzyme E2 D1 (UBE2D1) Ubiquitin-conjugating enzyme family P51668
Ubiquitin-conjugating enzyme E2 D4 (UBE2D4) Ubiquitin-conjugating enzyme family Q9Y2X8

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
3 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
4 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
5 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
6 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
7 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
8 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
9 Rapid Covalent-Probe Discovery by Electrophile-Fragment Screening. J Am Chem Soc. 2019 Jun 5;141(22):8951-8968. doi: 10.1021/jacs.9b02822. Epub 2019 May 22.