Details of the Target

General Information of Target

Target ID LDTP12719
Target Name Spermine oxidase (SMOX)
Gene Name SMOX
Gene ID 54498
Synonyms
C20orf16; SMO; Spermine oxidase; EC 1.5.3.16; Polyamine oxidase 1; PAO-1; PAOh1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEFAAENEGKSGGGLHSVAEGVRLSPEPGREGVRDLAGAEEFGGGEEGTGLTGIKEIGDG
EEGSGQRPEEIPMDLTVVKQEIIDWPGTEGRLAGQWVEQEVEDRPEVKDENAGVLEVKQE
TDSSLVVKEAKVGEPEVKEEKVKEEVMDWSEVKEEKDNLEIKQEEKFVGQCIKEELMHGE
CVKEEKDFLKKEIVDDTKVKEEPPINHPVGCKRKLAMSRCETCGTEEAKYRCPRCMRYSC
SLPCVKKHKAELTCNGVRDKTAYISIQQFTEMNLLSDYRFLEDVARTADHISRDAFLKRP
ISNKYMYFMKNRARRQGINLKLLPNGFTKRKENSTFFDKKKQQFCWHVKLQFPQSQAEYI
EKRVPDDKTINEILKPYIDPEKSDPVIRQRLKAYIRSQTGVQILMKIEYMQQNLVRYYEL
DPYKSLLDNLRNKVIIEYPTLHVVLKGSNNDMKVLHQVKSESTKNVGNEN
Target Bioclass
Enzyme
Family
Flavin monoamine oxidase family
Subcellular location
Cytoplasm
Function
Flavoenzyme which catalyzes the oxidation of spermine to spermidine. Can also use N(1)-acetylspermine and spermidine as substrates, with different affinity depending on the isoform (isozyme) and on the experimental conditions. Plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs. May contribute to beta-alanine production via aldehyde dehydrogenase conversion of 3-amino-propanal.
Uniprot ID
Q9NWM0
Ensemble ID
ENST00000278795.7
HGNC ID
HGNC:15862
ChEMBL ID
CHEMBL2176769

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 SNV: p.E227K .
AN3CA SNV: p.D141Y .
HCC1395 SNV: p.Q340K .
HCT15 SNV: p.L54F; p.A355D .
KASUMI2 SNV: p.R63H .
KYSE150 SNV: p.S108R .
LNCaP clone FGC SNV: p.A231V .
LS180 SNV: p.V315G .
MOLT4 SNV: p.D157N .
NALM6 SNV: p.T485M .
NCIH2286 SNV: p.Q256K .
OCUM1 SNV: p.K203T .
OVK18 SNV: p.W404R .
TYKNU SNV: p.P416L .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C409(1.40)  LDD3421  [1]
IPM
 Probe Info  		N.A.  LDD0147  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 Heraklio 02 ER- C409(1.46)  LDD2401  [1]
 LDCM0023  KB03 HCC15 C409(1.40)  LDD2757  [1]
 LDCM0024  KB05 SiSo C409(1.40)  LDD3421  [1]

The Interaction Atlas With This Target

The Drug(s) Related To This Target

Investigative
Click To Hide/Show 2 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Spermine Small molecular drug DB00127
Mdl72527 . DB04188

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255