Details of the Target

General Information of Target

Target ID LDTP12594
Target Name 1-acylglycerol-3-phosphate O-acyltransferase PNPLA3 (PNPLA3)
Gene Name PNPLA3
Gene ID 80339
Synonyms
ADPN; C22orf20; 1-acylglycerol-3-phosphate O-acyltransferase PNPLA3; EC 2.3.1.51; Acylglycerol transacylase; Adiponutrin; ADPN; Calcium-independent phospholipase A2-epsilon; iPLA2-epsilon; EC 3.1.1.4; Lysophosphatidic acid acyltransferase; Patatin-like phospholipase domain-containing protein 3; EC 3.1.1.3
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDFRTACEETKTGICLLQDGNQEPFKVRLHLAKDILMIQEQDVICVSGEPFYSGERTVTI
RRQTVGGFGLSIKGGAEHNIPVVVSKISKEQRAELSGLLFIGDAILQINGINVRKCRHEE
VVQVLRNAGEEVTLTVSFLKRAPAFLKLPLNEDCACAPSDQSSGTSSPLCDSGLHLNYHP
NNTDTLSCSSWPTSPGLRWEKRWCDLRLIPLLHSRFSQYVPGTDLSRQNAFQVIAVDGVC
TGIIQCLSAEDCVDWLQAIATNISNLTKHNIKKINRNFPVNQQIVYMGWCEAREQDPLQD
RVYSPTFLALRGSCLYKFLAPPVTTWDWTRAEKTFSVYEIMCKILKDSDLLDRRKQCFTV
QSESGEDLYFSVELESDLAQWERAFQTATFLEVERIQCKTYACVLESHLMGLTIDFSTGF
ICFDAATKAVLWRYKFSQLKGSSDDGKSKIKFLFQNPDTKQIEAKELEFSNLFAVLHCIH
SFFAAKVACLDPLFLGNQATASTAASSATTSKAKYTT
Target Type
Clinical trial
Target Bioclass
Enzyme
Subcellular location
Membrane
Function
Specifically catalyzes coenzyme A (CoA)-dependent acylation of 1-acyl-sn-glycerol 3-phosphate (2-lysophosphatidic acid/LPA) to generate phosphatidic acid (PA), an important metabolic intermediate and precursor for both triglycerides and glycerophospholipids. Does not esterify other lysophospholipids. Acyl donors are long chain (at least C16) fatty acyl-CoAs: arachidonoyl-CoA, linoleoyl-CoA, oleoyl-CoA and at a lesser extent palmitoyl-CoA. Additionally possesses low triacylglycerol lipase and CoA-independent acylglycerol transacylase activities and thus may play a role in acyl-chain remodeling of triglycerides. In vitro may express hydrolytic activity against glycerolipids triacylglycerol, diacylglycerol and monoacylglycerol, with a strong preference for oleic acid as the acyl moiety. However, the triacylglycerol hydrolase activity is controversial and may be very low. Possesses phospholipase A2 activity.
TTD ID
T63214
Uniprot ID
Q9NST1
DrugMap ID
TTEUAEH
Ensemble ID
ENST00000216180.8
HGNC ID
HGNC:18590

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCT15 SNV: p.L481M .
KMS11 SNV: p.Q96R .
MEWO SNV: p.S9C; p.S9R .
RT112 SNV: p.Y242Ter .
TOV21G SNV: p.D381N .
YSCCC SNV: p.F159L .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IPM
 Probe Info  		C400(3.64)  LDD2229  [1]

References

1 A quantitative thiol reactivity profiling platform to analyze redox and electrophile reactive cysteine proteomes. Nat Protoc. 2020 Sep;15(9):2891-2919. doi: 10.1038/s41596-020-0352-2. Epub 2020 Jul 20.
Mass spectrometry data entry: PXD016048