Details of the Target

General Information of Target

Target ID LDTP12466
Target Name Protein arginine N-methyltransferase 8 (PRMT8)
Gene Name PRMT8
Gene ID 56341
Synonyms
HRMT1L3; HRMT1L4; Protein arginine N-methyltransferase 8; EC 2.1.1.319; Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 4
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MWEANPEMFHKAEELFSKTTNNEVDDMDTSDTQWGWFYLAECGKWHMFQPDTNSQCSVSS
EDIEKSFKTNPCGSISFTTSKFSYKIDFAEMKQMNLTTGKQRLIKRAPFSISAFSYICEN
EAIPMPPHWENVNTQVPYQLIPLHNQTHEYNEVANLFGKTMDRNRIKRIQRIQNLDLWEF
FCRKKAQLKKKRGVPQINEQMLFHGTSSEFVEAICIHNFDWRINGIHGAVFGKGTYFARD
AAYSSRFCKDDIKHGNTFQIHGVSLQQRHLFRTYKSMFLARVLIGDYINGDSKYMRPPSK
DGSYVNLYDSCVDDTWNPKIFVVFDANQIYPEYLIDFH
Target Bioclass
Enzyme
Family
Class I-like SAM-binding methyltransferase superfamily, Protein arginine N-methyltransferase family, PRMT8 subfamily
Subcellular location
Cell membrane
Function
S-adenosyl-L-methionine-dependent and membrane-associated arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA) in proteins such as NIFK, myelin basic protein, histone H4, H2A and H2A/H2B dimer. Able to mono- and dimethylate EWS protein; however its precise role toward EWS remains unclear as it still interacts with fully methylated EWS.
Uniprot ID
Q9NR22
Ensemble ID
ENST00000382622.4
HGNC ID
HGNC:5188
ChEMBL ID
CHEMBL3108648

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A431 SNV: p.Q52P .
CAOV3 SNV: p.R350M .
HGC27 Deletion: p.E352RfsTer9 .
IPC298 SNV: p.E88K .
KASUMI2 Insertion: p.Q35PfsTer7 .
MCC13 SNV: p.E343K; p.G351R .
SUPT1 SNV: p.A272V .
TFK1 SNV: p.D147E .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
ATP probe
 Probe Info  		N.A.  LDD0199  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein arginine N-methyltransferase 1 (PRMT1) Protein arginine N-methyltransferase family Q99873
Protein arginine N-methyltransferase 8 (PRMT8) Protein arginine N-methyltransferase family Q9NR22
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Heterogeneous nuclear ribonucleoprotein Q (SYNCRIP) . O60506
Ubiquitin-like protein 5 (UBL5) . Q9BZL1

References

1 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.