Details of the Target

General Information of Target

Target ID LDTP12421
Target Name Probable ATP-dependent RNA helicase DDX4 (DDX4)
Gene Name DDX4
Gene ID 54514
Synonyms
VASA; Probable ATP-dependent RNA helicase DDX4; EC 3.6.4.13; DEAD box protein 4; Vasa homolog
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MPWLLSAPKLVPAVANVRGLSGCMLCSQRRYSLQPVPERRIPNRYLGQPSPFTHPHLLRP
GEVTPGLSQVEYALRRHKLMSLIQKEAQGQSGTDQTVVVLSNPTYYMSNDIPYTFHQDNN
FLYLCGFQEPDSILVLQSLPGKQLPSHKAILFVPRRDPSRELWDGPRSGTDGAIALTGVD
EAYTLEEFQHLLPKMKAETNMVWYDWMRPSHAQLHSDYMQPLTEAKAKSKNKVRGVQQLI
QRLRLIKSPAEIERMQIAGKLTSQAFIETMFTSKAPVEEAFLYAKFEFECRARGADILAY
PPVVAGGNRSNTLHYVKNNQLIKDGEMVLLDGGCESSCYVSDITRTWPVNGRFTAPQAEL
YEAVLEIQRDCLALCFPGTSLENIYSMMLTLIGQKLKDLGIMKNIKENNAFKAARKYCPH
HVGHYLGMDVHDTPDMPRSLPLQPGMVITIEPGIYIPEDDKDAPEKFRGLGVRIEDDVVV
TQDSPLILSADCPKEMNDIEQICSQAS
Target Bioclass
Enzyme
Family
DEAD box helicase family, DDX4/VASA subfamily
Subcellular location
Cytoplasm
Function
ATP-dependent RNA helicase required during spermatogenesis. Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle. Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs.
Uniprot ID
Q9NQI0
Ensemble ID
ENST00000353507.9
HGNC ID
HGNC:18700

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A101D SNV: p.P165S .
ABC1 SNV: p.I538L .
CHL1 SNV: p.I322V .
COLO792 SNV: p.Y271C
Substitution: .		 .
HCC15 SNV: p.E118Q .
HDQP1 Deletion: p.P249LfsTer18 .
HT115 SNV: p.S318N .
JURKAT SNV: p.I399L .
MDST8 SNV: p.D24N .
MOLT4 SNV: p.R177H .
PC14 SNV: p.S113C .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Acrolein
 Probe Info  		N.A.  LDD0217  [1]
Cinnamaldehyde
 Probe Info  		N.A.  LDD0220  [1]
Crotonaldehyde
 Probe Info  		N.A.  LDD0219  [1]
Methacrolein
 Probe Info  		N.A.  LDD0218  [1]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STS-2
 Probe Info  		N.A.  LDD0138  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [1]
 LDCM0107  IAA HeLa N.A.  LDD0221  [1]

References

1 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
2 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.