Details of the Target

General Information of Target

Target ID LDTP12380
Target Name NLR family CARD domain-containing protein 4 (NLRC4)
Gene Name NLRC4
Gene ID 58484
Synonyms
CARD12; CLAN; CLAN1; IPAF; NLR family CARD domain-containing protein 4; CARD, LRR, and NACHT-containing protein; CED-4-like protein Clan; Caspase recruitment domain-containing protein 12; Ice protease-activating factor; Ipaf
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEVPPPAPRSFLCRALCLFPRVFAAEAVTADSEVLEERQKRLPYVPEPYYPESGWDRLRE
LFGKDEQQRISKDLANICKTAATAGIIGWVYGGIPAFIHAKQQYIEQSQAEIYHNRFDAV
QSAHRAATRGFIRYGWRWGWRTAVFVTIFNTVNTSLNVYRNKDALSHFVIAGAVTGSLFR
INVGLRGLVAGGIIGALLGTPVGGLLMAFQKYSGETVQERKQKDRKALHELKLEEWKGRL
QVTEHLPEKIESSLQEDEPENDAKKIEALLNLPRNPSVIDKQDKD
Target Bioclass
Other
Subcellular location
Cytoplasm
Function
Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis. The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria.
Uniprot ID
Q9NPP4
Ensemble ID
ENST00000342905.10
HGNC ID
HGNC:16412
ChEMBL ID
CHEMBL4630857

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
786O SNV: p.Q82Ter .
C32 SNV: p.L108F .
COLO792 SNV: p.G211R .
EFO27 SNV: p.V277A .
HCT15 SNV: p.T231I .
HS578T SNV: p.F961S .
LNCaP clone FGC SNV: p.K440N .
MEWO SNV: p.G86R; p.Q219R .
OPM2 SNV: p.D1011Y .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Acrolein
 Probe Info  		N.A.  LDD0217  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [1]
 LDCM0107  IAA HeLa N.A.  LDD0221  [1]
 LDCM0109  NEM HeLa N.A.  LDD0223  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Caspase-1 (CASP1) Peptidase C14A family P29466
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
NLR family CARD domain-containing protein 4 (NLRC4) . Q9NPP4

References

1 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.