Details of the Target

General Information of Target

Target ID LDTP12069
Target Name E3 ubiquitin-protein ligase TRIM45 (TRIM45)
Gene Name TRIM45
Gene ID 80263
Synonyms
RNF99; E3 ubiquitin-protein ligase TRIM45; EC 2.3.2.27; RING finger protein 99
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MVDRLANSEANTRRISIVENCFGAAGQPLTIPGRVLIGEGVLTKLCRKKPKARQFFLFND
ILVYGNIVIQKKKYNKQHIIPLENVTIDSIKDEGDLRNGWLIKTPTKSFAVYAATATEKS
EWMNHINKCVTDLLSKSGKTPSNEHAAVWVPDSEATVCMRCQKAKFTPVNRRHHCRKCGF
VVCGPCSEKRFLLPSQSSKPVRICDFCYDLLSAGDMATCQPARSDSYSQSLKSPLNDMSD
DDDDDDSSD
Target Bioclass
Enzyme
Family
TRIM/RBCC family
Subcellular location
Cytoplasm
Function
E3 ubiquitin-protein ligase that plays a role in the regulation of inflammatory response. Mechanistically, mediates the 'Lys-48'-linked polyubiquitination of TAB2, a regulatory protein of the kinase TAK1, leading to its degradation via the proteasomal pathway and inhibition of the TLR-mediated inflammatory immune response. May act as a transcriptional repressor in mitogen-activated protein kinase signaling pathway.
Uniprot ID
Q9H8W5
Ensemble ID
ENST00000256649.9
HGNC ID
HGNC:19018

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
JURKAT SNV: p.R502G .
KYSE510 SNV: p.K371N .
SW756 SNV: p.S2Ter .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C29(1.88); C32(1.88)  LDD0080  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 HCT 116 C29(1.88); C32(1.88)  LDD0080  [1]
 LDCM0023  KB03 HCT 116 C29(3.45); C32(3.45)  LDD0081  [1]
 LDCM0024  KB05 HCT 116 C29(1.40); C32(1.40)  LDD0082  [1]

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.