Details of the Target

General Information of Target

Target ID LDTP11932
Target Name F-box only protein 44 (FBXO44)
Gene Name FBXO44
Gene ID 93611
Synonyms
FBG3; FBX30; FBX44; FBX6A; FBXO6A; F-box only protein 44; F-box protein FBX30; F-box/G-domain protein 3
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MNLFNLDRFRFEKRNKIEEAPEATPQPSQPGPSSPISLSAEEENAEGEVSRANTPDSDIT
EKTEDSSVPETPDNERKASISYFKNQRGIQYIDLSSDSEDVVSPNCSNTVQEKTFNKDTV
IIVSEPSEDEESQGLPTMARRNDDISELEDLSELEDLKDAKLQTLKELFPQRSDNDLLKL
IESTSTMDGAIAAALLMFGDAGGGPRKRKLSSSSEPYEEDEFNDDQSIKKTRLDHGEESN
ESAESSSNWEKQESIVLKLQKEFPNFDKQELREVLKEHEWMYTEALESLKVFAEDQDMQY
VSQSEVPNGKEVSSRSQNYPKNATKTKLKQKFSMKAQNGFNKKRKKNVFNPKRVVEDSEY
DSGSDVGSSLDEDYSSGEEVMEDGYKGKILHFLQDASIGELTLIPQCSQKKAQKITELRP
FNSWEALFTKMSKTNGLSEDLIWHCKTLIQERDVVIRLMNKCEDISNKLTKQVTMLTGNG
GGWNIEQPSILNQSLSLKPYQKVGLNWLALVHKHGLNGILADEMGLGKTIQAIAFLAYLY
QEGNNGPHLIVVPASTIDNWLREVNLWCPTLKVLCYYGSQEERKQIRFNIHSRYEDYNVI
VTTYNCAISSSDDRSLFRRLKLNYAIFDEGHMLKNMGSIRYQHLMTINANNRLLLTGTPV
QNNLLELMSLLNFVMPHMFSSSTSEIRRMFSSKTKSADEQSIYEKERIAHAKQIIKPFIL
RRVKEEVLKQLPPKKDRIELCAMSEKQEQLYLGLFNRLKKSINNLEKNTEMCNVMMQLRK
MANHPLLHRQYYTAEKLKEMSQLMLKEPTHCEANPDLIFEDMEVMTDFELHVLCKQYRHI
NNFQLDMDLILDSGKFRVLGCILSELKQKGDRVVLFSQFTMMLDILEVLLKHHQHRYLRL
DGKTQISERIHLIDEFNTDMDIFVFLLSTKAGGLGINLTSANVVILHDIDCNPYNDKQAE
DRCHRVGQTKEVLVIKLISQGTIEESMLKINQQKLKLEQDMTTVDEGDEGSMPADIATLL
KTSMGL
Target Bioclass
Enzyme
Function Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
Uniprot ID
Q9H4M3
Ensemble ID
ENST00000251546.8
HGNC ID
HGNC:24847

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 SNV: p.R49C .
BT549 Substitution: p.E59K .
CCSW1 Substitution: p.E59K .
CHL1 SNV: p.Q179H .
HT115 SNV: p.S182L .
IPC298 Substitution: p.E59K .
LS513 Substitution: p.E59K .
NB4 Substitution: p.E59K .
NCIH358 Substitution: p.E59K .
OVCAR4 Substitution: p.E59K .
SUPT1 SNV: p.T232A .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C170(1.18)  LDD3464  [1]
BTD
 Probe Info  		C30(0.39)  LDD2104  [2]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [3]
NAIA_5
 Probe Info  		N.A.  LDD2223  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 A673 C170(0.98)  LDD2261  [1]
 LDCM0023  KB03 G-361 C170(1.58)  LDD2746  [1]
 LDCM0024  KB05 TC-71 C170(1.18)  LDD3464  [1]
 LDCM0511  Nucleophilic fragment 18b MDA-MB-231 C30(0.39)  LDD2104  [2]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C30(0.25)  LDD2135  [2]
 LDCM0627  NUDT7-COV-1 HEK-293T C30(1.00)  LDD2206  [5]
 LDCM0628  OTUB2-COV-1 HEK-293T C30(0.70)  LDD2207  [5]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Maspardin (SPG21) AB hydrolase superfamily Q9NZD8
S-phase kinase-associated protein 1 (SKP1) SKP1 family P63208
Other
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Biogenesis of lysosome-related organelles complex 1 subunit 1 (BLOC1S1) BLOC1S1 family P78537
BLOC-1-related complex subunit 6 (BORCS6) BORCS6 family Q96GS4
Heat shock factor 2-binding protein (HSF2BP) . O75031
SERTA domain-containing protein 3 (SERTAD3) . Q9UJW9
Uncharacterized protein KCNJ5-AS1 (KCNJ5-AS1) . Q8TAV5

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
3 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
5 Rapid Covalent-Probe Discovery by Electrophile-Fragment Screening. J Am Chem Soc. 2019 Jun 5;141(22):8951-8968. doi: 10.1021/jacs.9b02822. Epub 2019 May 22.