Details of the Target

General Information of Target

Target ID LDTP11741
Target Name Ubiquitin-like-conjugating enzyme ATG10 (ATG10)
Gene Name ATG10
Gene ID 83734
Synonyms
APG10L; Ubiquitin-like-conjugating enzyme ATG10; EC 2.3.2.-; Autophagy-related protein 10; APG10-like
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MKEEAFLRRRFSLCPPSSTPQKVDPRKLTRNLLLSGDNELYPLSPGKDMEPNGPSLPRDE
GPPTPSSATKVPPAEYRLCNGSDKECVSPTARVTKKETLKAQKENYRQEKKRATRQLLSA
LTDPSVVIMADSLKIRGTLKSWTKLWCVLKPGVLLIYKTPKVGQWVGTVLLHCCELIERP
SKKDGFCFKLFHPLDQSVWAVKGPKGESVGSITQPLPSSYLIFRAASESDGRCWLDALEL
ALRCSSLLRLGTCKPGRDGEPGTSPDASPSSLCGLPASATVHPDQDLFPLNGSSLENDAF
SDKSERENPEESDTETQDHSRKTESGSDQSETPGAPVRRGTTYVEQVQEELGELGEASQV
ETVSEENKSLMWTLLKQLRPGMDLSRVVLPTFVLEPRSFLNKLSDYYYHADLLSRAAVEE
DAYSRMKLVLRWYLSGFYKKPKGIKKPYNPILGETFRCCWFHPQTDSRTFYIAEQVSHHP
PVSAFHVSNRKDGFCISGSITAKSRFYGNSLSALLDGKATLTFLNRAEDYTLTMPYAHCK
GILYGTMTLELGGKVTIECAKNNFQAQLEFKLKPFFGGSTSINQISGKITSGEEVLASLS
GHWDRDVFIKEEGSGSSALFWTPSGEVRRQRLRQHTVPLEEQTELESERLWQHVTRAISK
GDQHRATQEKFALEEAQRQRARERQESLMPWKPQLFHLDPITQEWHYRYEDHSPWDPLKD
IAQFEQDGILRTLQQEAVARQTTFLGSPGPRHERSGPDQRLRKASDQPSGHSQATESSGS
TPESCPELSDEEQDGDFVPGGESPCPRCRKEARRLQALHEAILSIREAQQELHRHLSAML
SSTARAAQAPTPGLLQSPRSWFLLCVFLACQLFINHILK
Target Bioclass
Enzyme
Family
ATG10 family
Subcellular location
Cytoplasm
Function
E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3. Plays a role in adenovirus-mediated cell lysis.
Uniprot ID
Q9H0Y0
Ensemble ID
ENST00000282185.8
HGNC ID
HGNC:20315

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
ABC1 SNV: p.L163P .
MCC13 SNV: p.D28N .
MKN45 SNV: p.V175I .
RL952 Deletion: p.L118Ter .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IA-alkyne
 Probe Info  		C166(20.00)  LDD1704  [1]
DBIA
 Probe Info  		C38(1.50)  LDD0080  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 HCT 116 C38(1.50)  LDD0080  [2]
 LDCM0023  KB03 HCT 116 C38(1.61)  LDD0081  [2]
 LDCM0024  KB05 HCT 116 C38(1.65)  LDD0082  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Caspase-6 (CASP6) Peptidase C14A family P55212
Atypical kinase COQ8A, mitochondrial (COQ8A) ADCK protein kinase family Q8NI60
GTP-binding nuclear protein Ran (RAN) Ran family P62826
Transporter and channel
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Huntingtin-interacting protein 1 (HIP1) SLA2 family O00291
Alpha-synuclein (SNCA) Synuclein family P37840
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2 (GNB2) WD repeat G protein beta family P62879
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Autophagy protein 5 (ATG5) ATG5 family Q9H1Y0
Ataxin-1 (ATXN1) ATXN1 family P54253
RING finger protein 11 (RNF11) . Q9Y3C5

References

1 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
2 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.