Details of the Target

General Information of Target

Target ID LDTP11727
Target Name Guanylate-binding protein 3 (GBP3)
Gene Name GBP3
Gene ID 2635
Synonyms
Guanylate-binding protein 3; EC 3.6.5.-; GTP-binding protein 3; GBP-3; Guanine nucleotide-binding protein 3
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAEAEGSSLLLLPPPPPPPRMAEVEAPTAAETDMKQYQGSGGVAMDVERSRFPYCVVWTP
IPVLTWFFPIIGHMGICTSTGVIRDFAGPYFVSEDNMAFGKPAKYWKLDPAQVYASGPNA
WDTAVHDASEEYKHRMHNLCCDNCHSHVALALNLMRYNNSTNWNMVTLCFFCLLYGKYVS
VGAFVKTWLPFILLLGIILTVSLVFNLR
Target Bioclass
Enzyme
Family
TRAFAC class dynamin-like GTPase superfamily, GB1/RHD3 GTPase family, GB1 subfamily
Subcellular location
Cytoplasm
Function
Interferon (IFN)-inducible GTPase that plays important roles in innate immunity against a diverse range of bacterial, viral and protozoan pathogens. Hydrolyzes GTP very efficiently; GDP rather than GMP is the major reaction product. Following infection, recruited to the pathogen-containing vacuoles or vacuole-escaped bacteria and acts as a positive regulator of inflammasome assembly by promoting the release of inflammasome ligands from bacteria. Acts by promoting lysis of pathogen-containing vacuoles, releasing pathogens into the cytosol. Following pathogen release in the cytosol, promotes recruitment of proteins that mediate bacterial cytolysis: this liberates ligands that are detected by inflammasomes, such as lipopolysaccharide (LPS) that activates the non-canonical CASP4/CASP11 inflammasome or double-stranded DNA (dsDNA) that activates the AIM2 inflammasome. Exhibits antiviral activity against influenza virus.; [Isoform 2]: Shows the most prominent antiviral activity in epithelial cells.
Uniprot ID
Q9H0R5
Ensemble ID
ENST00000370481.9
HGNC ID
HGNC:4184

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAL148 Substitution: p.L126F .
HUH1 Deletion: p.S184PfsTer21
SNV: p.S184Y		 .
NALM6 SNV: p.R557Ter .
NCIH146 SNV: p.L54V .
TE4 SNV: p.E456Ter .
WM2664 Insertion: p.H85SfsTer13 .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IPM
 Probe Info  		C394(0.72)  LDD1702  [1]
IA-alkyne
 Probe Info  		C405(0.00); C268(0.00)  LDD0166  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C394(0.72)  LDD1702  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4) STE Ser/Thr protein kinase family O95819
Guanylate-binding protein 1 (GBP1) GB1/RHD3 GTPase family P32455
Guanylate-binding protein 2 (GBP2) GB1/RHD3 GTPase family P32456
Guanylate-binding protein 3 (GBP3) GB1/RHD3 GTPase family Q9H0R5
Guanylate-binding protein 5 (GBP5) GB1/RHD3 GTPase family Q96PP8

References

1 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
2 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060