Details of the Target

General Information of Target

Target ID LDTP11603
Target Name Centrosomal protein of 295 kDa (CEP295)
Gene Name CEP295
Gene ID 85459
Synonyms
KIAA1731; Centrosomal protein of 295 kDa
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDYPKMDYFLDVESAHRLLDVESAQRFFYSQGAQARRATLLLPPTLMAASSEDDIDRRPI
RRVRSKSDTPYLAEARISFNLGAAEEVERLAAMRSDSLVPGTHTPPIRRRSKFANLGRIF
KPWKWRKKKSEKFKHTSAALERKISMRQSREELIKRGVLKEIYDKDGELSISNEEDSLEN
GQSLSSSQLSLPALSEMEPVPMPRDPCSYEVLQPSDIMDGPDPGAPVKLPCLPVKLSPPL
PPKKVMICMPVGGPDLSLVSYTAQKSGQQGVAQHHHTVLPSQIQHQLQYGSHGQHLPSTT
GSLPMHPSGCRMIDELNKTLAMTMQRLESSEQRVPCSTSYHSSGLHSGDGVTKAGPMGLP
EIRQVPTVVIECDDNKENVPHESDYEDSSCLYTREEEEEEEDEDDDSSLYTSSLAMKVCR
KDSLAIKLSNRPSKRELEEKNILPRQTDEERLELRQQIGTKLTRRLSQRPTAEELEQRNI
LKPRNEQEEQEEKREIKRRLTRKLSQRPTVEELRERKILIRFSDYVEVADAQDYDRRADK
PWTRLTAADKAAIRKELNEFKSTEMEVHELSRHLTRFHRP
Target Bioclass
Other
Subcellular location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole
Function
Centriole-enriched microtubule-binding protein involved in centriole biogenesis. Essential for the generation of the distal portion of new-born centrioles in a CENPJ- and CEP120-mediated elongation dependent manner during the cell cycle S/G2 phase after formation of the initiating cartwheel structure. Required for the recruitment of centriolar proteins, such as POC1B, POC5 and CEP135, into the distal portion of centrioles. Also required for centriole-to-centrosome conversion during mitotic progression, but is dispensable for cartwheel removal or centriole disengagement. Binds to and stabilizes centriolar microtubule.
Uniprot ID
Q9C0D2
Ensemble ID
ENST00000325212.11
HGNC ID
HGNC:29366

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
8505C SNV: p.S2295G .
A3KAW Substitution: p.T864R .
ABC1 SNV: p.T916I .
C4II SNV: p.S461R .
HCC1187 SNV: p.L1096M .
HCC15 Substitution: p.T864R .
HCC44 SNV: p.W1263L .
HCT15 SNV: p.A1368S .
HT115 SNV: p.K858N; p.E1363Ter .
HUH7 Substitution: p.T864R .
ICC3 SNV: p.N2302K .
IGR1 SNV: p.P1657S .
JHH6 Substitution: p.T864R .
KATOIII Substitution: p.T864R .
KMCH1 Substitution: p.T864R .
KNS81FD Substitution: p.T864R .
KPL1 SNV: p.E179V .
KPNYN Substitution: p.T864R .
LN18 SNV: p.P1314S .
MDAMB157 SNV: p.G2376A .
MDAMB453 SNV: p.D2148H .
MELJUSO SNV: p.L46S; p.K2488E .
MEWO SNV: p.P1230L; p.P1471S .
MKN1 Substitution: p.T864R .
MOLT4 Deletion: p.K2362NfsTer5 .
NOMO1 Substitution: p.T864R .
NUGC3 SNV: p.Q1171H .
OCUG1 Substitution: p.T864R .
OSRC2 Substitution: p.T864R .
OVISE Substitution: p.T864R .
P31FUJ Substitution: p.T864R .
RKO SNV: p.R395Ter; p.T470A .
SISO Substitution: p.T864R .
SKMEL24 SNV: p.E444K .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K398(7.06)  LDD0277  [1]
DBIA
 Probe Info  		C540(22.63)  LDD0209  [2]
NAIA_4
 Probe Info  		N.A.  LDD2226  [3]
IPM
 Probe Info  		N.A.  LDD2156  [4]
TFBX
 Probe Info  		N.A.  LDD0148  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0023  KB03 Jurkat C540(22.63)  LDD0209  [2]

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
3 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
4 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
5 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255