Details of the Target

General Information of Target

Target ID LDTP11446
Target Name Dual specificity protein phosphatase 16 (DUSP16)
Gene Name DUSP16
Gene ID 80824
Synonyms
KIAA1700; MKP7; Dual specificity protein phosphatase 16; EC 3.1.3.16; EC 3.1.3.48; Mitogen-activated protein kinase phosphatase 7; MAP kinase phosphatase 7; MKP-7
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MKDFSDVILCMEATESSKTEFCNPAFEPESGPPCPPPVFPEDASYSVPAPWHGRRPRGLR
PDCRFSWLCVLLLSSLLLLLLGLLVAIILAQLQAAPPSGASHSPLPAGGLTTTTTTPTIT
TSQAAGTPKGQQESGVSPSPQSTCGGLLSGPRGFFSSPNYPDPYPPNTHCVWHIQVATDH
AIQLKIEALSIESVASCLFDRLELSPEPEGPLLRVCGRVPPPTLNTNASHLLVVFVSDSS
VEGFGFHAWYQAMAPGRGSCAHDEFRCDQLICLLPDSVCDGFANCADGSDETNCSAKFSG
CGGNLTGLQGTFSTPSYLQQYPHQLLCTWHISVPAGHSIELQFHNFSLEAQDECKFDYVE
VYETSSSGAFSLLGRFCGAEPPPHLVSSHHELAVLFRTDHGISSGGFSATYLAFNATENP
CGPSELSCQAGGCKGVQWMCDMWRDCTDGSDDNCSGPLFPPPELACEPVQVEMCLGLSYN
TTAFPNIWVGMITQEEVVEVLSGYKSLTSLPCYQHFRRLLCGLLVPRCTPLGSVLPPCRS
VCQEAEHQCQSGLALLGTPWPFNCNRLPEAADLEACAQP
Target Bioclass
Enzyme
Family
Protein-tyrosine phosphatase family, Non-receptor class dual specificity subfamily
Subcellular location
Cytoplasm
Function Dual specificity protein phosphatase involved in the inactivation of MAP kinases. Dephosphorylates MAPK10 bound to ARRB2.
Uniprot ID
Q9BY84
Ensemble ID
ENST00000228862.3
HGNC ID
HGNC:17909

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CCK81 SNV: p.K397E .
CCLP1 SNV: p.L220F .
CMK SNV: p.E644G .
FTC133 SNV: p.R483Ter .
HCT15 SNV: p.S516R .
IM95 Deletion: p.R606SfsTer2 .
LN229 SNV: p.A2V .
LNCaP clone FGC Deletion: p.S99QfsTer43
SNV: p.M1?		 .
NCIH446 SNV: p.A350V .
OAW28 SNV: p.L499R .
RKO SNV: p.F445L .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C170(1.66)  LDD3444  [1]
IA-alkyne
 Probe Info  		C216(5.30)  LDD1704  [2]
IPM
 Probe Info  		N.A.  LDD0147  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 T cell-activated C216(5.30)  LDD1704  [2]
 LDCM0023  KB03 SNU-478 C170(9.42)  LDD3027  [1]
 LDCM0024  KB05 SNU-478 C170(1.66)  LDD3444  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Mitogen-activated protein kinase 9 (MAPK9) CMGC Ser/Thr protein kinase family P45984
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Wolframin (WFS1) . O76024

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
3 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255